{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T10:43:41Z","timestamp":1788864221045,"version":"build-2803163510"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T00:00:00Z","timestamp":1665532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100015466","name":"Milken Institute School of Public Health,","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100015466","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007108","name":"George Washington University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007108","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>During the 2022 monkeypox (MPX) outbreak, testing has been limited and results delayed, allowing ongoing transmission. Gold-standard quantitative polymerase chain reaction (qPCR) diagnostics are difficult to obtain. This research adapted the June 2022 CDC MPX qPCR assay for broad implementation. Validated using MPX stocks in a matrix with multiple sample types, MPX was detected with cycle threshold (Ct) values 17.46\u201335.59 and titer equivalents 8.01 \u00d7 106 to 2.45 \u00d7 100 plaque\u2013forming unit (PFU)\/mL. The detection limit was 3.59 PFU\/mL. Sensitivity and specificity were both 100%. This qPCR assay can be quickly and broadly implemented in research and public health laboratories to increase diagnostic capacity amid the growing MPX outbreak.<\/jats:p>","DOI":"10.1093\/infdis\/jiac414","type":"journal-article","created":{"date-parts":[[2022,10,10]],"date-time":"2022-10-10T21:19:46Z","timestamp":1665436786000},"page":"1084-1087","source":"Crossref","is-referenced-by-count":11,"title":["Development of an Accessible and Scalable Quantitative Polymerase Chain Reaction Assay for Monkeypox Virus Detection"],"prefix":"10.1093","volume":"227","author":[{"given":"Abigail J","family":"Porzucek","sequence":"first","affiliation":[{"name":"Department of Global Health, Milken Institute School of Public Health, George Washington University , Washington, District of Columbia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigale M","family":"Proctor","sequence":"additional","affiliation":[{"name":"Department of Global Health, Milken Institute School of Public Health, George Washington University , Washington, District of Columbia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina E","family":"Klinkhammer","sequence":"additional","affiliation":[{"name":"Department of Global Health, Milken Institute School of Public Health, George Washington University , Washington, District of Columbia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah R","family":"Tritsch","sequence":"additional","affiliation":[{"name":"Department of Global Health, Milken Institute School of Public Health, George Washington University , Washington, District of Columbia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly A","family":"Robertson","sequence":"additional","affiliation":[{"name":"Department of Global Health, Milken Institute School of Public Health, George Washington University , Washington, District of Columbia , 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6678-9049","authenticated-orcid":false,"given":"Christopher N","family":"Mores","sequence":"additional","affiliation":[{"name":"Department of Global Health, Milken Institute School of Public Health, George Washington University , Washington, District of Columbia , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,10,12]]},"reference":[{"key":"2023042618003339500_jiac414-B1","author":"World Health Organization"},{"key":"2023042618003339500_jiac414-B2","author":"US Department of Health and Human Services"},{"key":"2023042618003339500_jiac414-B3","author":"Centers for Disease Control and Prevention"},{"key":"2023042618003339500_jiac414-B4","author":"Barone","year":"2020"},{"key":"2023042618003339500_jiac414-B5","doi-asserted-by":"crossref","first-page":"e452","DOI":"10.1016\/S2468-2667(20)30157-2","article-title":"Impact of 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