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We used the recently optimized Plasmodium cynomolgi Berok in vitro culture model to investigate the putative P. vivax drug resistance marker MDR1 Y976F. Introduction of this mutation using clustered regularly interspaced short palindromic repeats\u2013CRISPR-associated protein 9 (CRISPR-Cas9) increased sensitivity to mefloquine, but had no significant effect on sensitivity to chloroquine, amodiaquine, piperaquine, and artesunate. To our knowledge, this is the first reported use of CRISPR-Cas9 in P. cynomolgi, and the first reported integrative genetic manipulation of this species.<\/jats:p>","DOI":"10.1093\/infdis\/jiac469","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T13:33:43Z","timestamp":1670420023000},"page":"1121-1126","source":"Crossref","is-referenced-by-count":11,"title":["Integrative Genetic Manipulation of <i>Plasmodium cynomolgi<\/i> Reveals Multidrug Resistance-1 Y976F Associated With Increased In Vitro Susceptibility to Mefloquine"],"prefix":"10.1093","volume":"227","author":[{"given":"Kurt E","family":"Ward","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Columbia University Irving Medical Center , New York, New York , USA"},{"name":"Department of Microbiology and Immunology, University of Otago , Dunedin , New 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