{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:43:00Z","timestamp":1791434580475,"version":"4.3.3"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T00:00:00Z","timestamp":1675382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["K01AI136725"],"award-info":[{"award-number":["K01AI136725"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["F32AI145157"],"award-info":[{"award-number":["F32AI145157"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"STAR Sexually Transmitted Infections Clinical Trials Group","award":["HHSN272201300014I\/HHSN27200011"],"award-info":[{"award-number":["HHSN272201300014I\/HHSN27200011"]}]},{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","award":["T32 MH080634"],"award-info":[{"award-number":["T32 MH080634"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000061","name":"Fogarty International Center","doi-asserted-by":"publisher","award":["K01TW012170"],"award-info":[{"award-number":["K01TW012170"]}],"id":[{"id":"10.13039\/100000061","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Neisseria gonorrhoeae is a major public health problem due to increasing incidence and antimicrobial resistance. Genetic markers of reduced susceptibility have been identified; the extent to which those are representative of global antimicrobial resistance is unknown. We evaluated the performance of whole-genome sequencing (WGS) used to predict susceptibility to ciprofloxacin and other antimicrobials using a global collection of N. gonorrhoeae isolates.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Susceptibility testing of common antimicrobials and the recently developed zolifodacin was performed using agar dilution to determine minimum inhibitory concentrations (MICs). We identified resistance alleles at loci known to contribute to antimicrobial resistance in N. gonorrhoeae from WGS data. We tested the ability of each locus to predict antimicrobial susceptibility.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>A total of 481 N. gonorrhoeae isolates, collected between 2004 and 2019 and making up 457 unique genomes, were sourced from 5 countries. All isolates with demonstrated susceptibility to ciprofloxacin (MIC \u22640.06 \u03bcg\/mL) had a wild-type gyrA codon 91. Multilocus approaches were needed to predict susceptibility to other antimicrobials. All isolates were susceptible to zoliflodacin, defined by an MIC \u22640.25 \u03bcg\/mL.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Single marker prediction can be used to inform ciprofloxacin treatment of N. gonorrhoeae infection. A combination of molecular markers may be needed to determine susceptibility for other antimicrobials.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiad027","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T03:23:16Z","timestamp":1675308196000},"page":"917-925","source":"Crossref","is-referenced-by-count":25,"title":["Whole-Genome Sequencing to Predict Antimicrobial Susceptibility Profiles in <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1093","volume":"227","author":[{"given":"Claire C","family":"Bristow","sequence":"first","affiliation":[{"name":"Department of Medicine, University of California San Diego , La Jolla, California , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6255-690X","authenticated-orcid":false,"given":"Tatum D","family":"Mortimer","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health , Boston, Massachusetts , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheldon","family":"Morris","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California San Diego , La Jolla, California , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5646-1314","authenticated-orcid":false,"given":"Yonatan H","family":"Grad","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health , Boston, Massachusetts , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olusegun O","family":"Soge","sequence":"additional","affiliation":[{"name":"Departments of Global Health, Allergy and Infectious Disease, Laboratory Medicine and Pathology, University of Washington , Seattle, Washington , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Wakatake","sequence":"additional","affiliation":[{"name":"Departments of Global Health, Allergy and Infectious Disease, Laboratory Medicine and Pathology, University of Washington , Seattle, Washington , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rushlenne","family":"Pascual","sequence":"additional","affiliation":[{"name":"Departments of Global Health, Allergy and Infectious Disease, Laboratory Medicine and Pathology, University of Washington , Seattle, Washington , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara McCurdy","family":"Murphy","sequence":"additional","affiliation":[{"name":"Social & Scientific Systems, a DLH Holdings Company , Silver Spring, Maryland , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyra E","family":"Fryling","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health , Boston, Massachusetts , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul C","family":"Adamson","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases at the David Geffen School of Medicine, University of California , Los Angeles, California , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4351-666X","authenticated-orcid":false,"given":"Jo-Anne","family":"Dillon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan , Saskatoon, Sikkim , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidhi R","family":"Parmar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan , Saskatoon, Sikkim , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai Ha Long","family":"Le","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Mycology and Parasitology, National Hospital of Venereology and Dermatology , Hanoi , Vietnam"},{"name":"Department of Clinical Microbiology and Parasitology, Hanoi Medical University , Hanoi , Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung","family":"Van Le","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Mycology and Parasitology, National Hospital of Venereology and Dermatology , Hanoi , Vietnam"},{"name":"Department of Dermatology and Venereology, Hanoi Medical University , Hanoi , Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reyna Margarita","family":"Ovalles Ure\u00f1a","sequence":"additional","affiliation":[{"name":"Laboratorio Nacional Dr. Defillo , Santo Domingo , Dominican 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