{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T06:16:15Z","timestamp":1774678575106,"version":"3.50.1"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"Supplement_7","license":[{"start":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T00:00:00Z","timestamp":1682726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001691","name":"KAKENHI","doi-asserted-by":"publisher","award":["20H03140"],"award-info":[{"award-number":["20H03140"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"KAKENHI","doi-asserted-by":"publisher","award":["21H02734"],"award-info":[{"award-number":["21H02734"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Grant-in-Aid for Scientific Research"},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Japanese Initiative for Progress of Research on Infectious Disease for Global Epidemics","award":["JP18fm0208101"],"award-info":[{"award-number":["JP18fm0208101"]}]},{"name":"Japanese Initiative for Progress of Research on Infectious Disease for Global Epidemics","award":["JP19fm0208001"],"award-info":[{"award-number":["JP19fm0208001"]}]},{"DOI":"10.13039\/501100009037","name":"Science and Technology Research Partnership for Sustainable Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009037","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009037","name":"(SATREPS)","doi-asserted-by":"crossref","award":["JP22jm0110019"],"award-info":[{"award-number":["JP22jm0110019"]}],"id":[{"id":"10.13039\/501100009037","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP20am0101114"],"award-info":[{"award-number":["JP20am0101114"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Platform Project for Supporting Drug Discovery and Life Science Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Our previous study demonstrated that the fruit bat (Yaeyama flying fox)-derived cell line FBKT1 showed preferential susceptibility to Ebola virus (EBOV), whereas the human cell line HEK293T was similarly susceptible to EBOV and Marburg virus (MARV). This was due to 3 amino acid differences of the endosomal receptor Niemann-Pick C1 (NPC1) between FBKT1 and HEK293T (ie, TET and SGA, respectively, at positions 425\u2013427), as well as 2 amino acid differences at positions 87 and 142 of the viral glycoprotein (GP) between EBOV and MARV.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods\/Results<\/jats:title>\n                  <jats:p>To understand the contribution of these amino acid differences to interactions between NPC1 and GP, we performed molecular dynamics simulations and binding free energy calculations. The average binding free energies of human NPC1 (hNPC1) and its mutant having TET at positions 425\u2013427 (hNPC1\/TET) were similar for the interaction with EBOV GP. In contrast, hNPC1\/TET had a weaker interaction with MARV GP than wild-type hNPC1. As expected, substitutions of amino acid residues at 87 or 142 in EBOV and MARV GPs converted the binding affinity to hNPC1\/TET.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our data provide structural and energetic insights for understanding potential differences in the GP-NPC1 interaction, which could influence the host tropism of EBOV and MARV.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiad120","type":"journal-article","created":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T15:00:39Z","timestamp":1682780439000},"page":"S479-S487","source":"Crossref","is-referenced-by-count":6,"title":["Structural and Energetic Basis for Differential Binding of Ebola and Marburg Virus Glycoproteins to a Bat-Derived Niemann-Pick C1 Protein"],"prefix":"10.1093","volume":"228","author":[{"given":"Manabu","family":"Igarashi","sequence":"first","affiliation":[{"name":"Division of Global Epidemiology, International Institute for Zoonosis Control, Hokkaido University , Sapporo, Hokkaido , Japan"},{"name":"International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University , Sapporo, Hokkaido , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takatsugu","family":"Hirokawa","sequence":"additional","affiliation":[{"name":"Transborder Medical Research Center, University of Tsukuba , Tsukuba, Ibaraki , Japan"},{"name":"Division of Biomedical Science, University of Tsukuba , Tsukuba, Ibaraki , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayato","family":"Takada","sequence":"additional","affiliation":[{"name":"Division of Global Epidemiology, International Institute for Zoonosis Control, Hokkaido University , Sapporo, Hokkaido , 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