{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T09:06:23Z","timestamp":1740128783109,"version":"3.37.3"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"Supplement_7","funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Although significant progress has been made in the development of therapeutics against Ebola virus (EBOV), we sought to expand upon existing strategies and combine an RNA interference\u2013based intervention with the approved vesicular stomatitis virus\u2013based Ebola virus (VSV-EBOV) vaccine to conjointly treat and vaccinate patients during an outbreak. We constructed VSV-EBOV vectors expressing artificial micro-RNAs (amiRNAs) targeting sequences of EBOV proteins. In vitro experiments demonstrated a robust decrease in EBOV replication using a minigenome system and infectious virus. For in vivo evaluation, mouse-adapted EBOV\u2013infected CD-1 mice were treated 24 hours after infection with a single dose of the VSV-EBOV amiRNA constructs. We observed no difference in disease progression or survival compared to the control-treated mice. In summary, while amiRNAs decrease viral replication in vitro, the effect is not sufficient to protect mice from lethal disease, and this therapeutic approach requires further optimization.<\/jats:p>","DOI":"10.1093\/infdis\/jiad121","type":"journal-article","created":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T08:06:23Z","timestamp":1682582783000},"page":"S677-S681","source":"Crossref","is-referenced-by-count":1,"title":["Single-Dose Treatment With Vesicular Stomatitis Virus\u2013Based Ebola Virus Vaccine Expressing Ebola Virus\u2013Specific Artificial Micro-RNA Does Not Protect Mice From Lethal Disease"],"prefix":"10.1093","volume":"228","author":[{"given":"Kyle L","family":"O\u2019Donnell","sequence":"first","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, Montana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Callison","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, Montana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9448-8227","authenticated-orcid":false,"given":"Heinz","family":"Feldmann","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, Montana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5829-6305","authenticated-orcid":false,"given":"Thomas","family":"Hoenen","sequence":"additional","affiliation":[{"name":"Laboratory for Integrative Cell and Infection Biology, Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut , Greifswald , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Marzi","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, Montana"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,4,27]]},"reference":[{"issue":"Suppl 1","key":"2023111521041250300_jiad121-B1","doi-asserted-by":"crossref","first-page":"S259","DOI":"10.1086\/514302","article-title":"Ebola outbreak in Kikwit, Democratic Republic of the Congo: discovery and control measures","volume":"179","author":"Muyembe-Tamfum","year":"1999","journal-title":"J Infect Dis"},{"year":"2022","author":"Centers for Disease Control and Prevention","key":"2023111521041250300_jiad121-B2"},{"key":"2023111521041250300_jiad121-B3","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.coi.2021.03.008","article-title":"Ebola virus disease: current vaccine solutions","volume":"71","author":"Tomori","year":"2021","journal-title":"Curr Opin Immunol"},{"key":"2023111521041250300_jiad121-B4","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.cell.2020.03.011","article-title":"A vaccine against Ebola virus","volume":"181","author":"Ollmann Saphire","year":"2020","journal-title":"Cell"},{"key":"2023111521041250300_jiad121-B5","first-page":"79","article-title":"Immunotherapeutics for Ebola virus disease: hope on the horizon","volume":"15","author":"O\u2019Donnell","year":"2021","journal-title":"Biologics"},{"key":"2023111521041250300_jiad121-B6","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.ebiom.2019.09.055","article-title":"Single low-dose VSV-EBOV vaccination protects cynomolgus macaques from lethal Ebola challenge","volume":"49","author":"Marzi","year":"2019","journal-title":"EBioMedicine"},{"key":"2023111521041250300_jiad121-B7","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1126\/science.aab3920","article-title":"Ebola vaccine. 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