{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T05:36:44Z","timestamp":1773466604236,"version":"3.50.1"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T00:00:00Z","timestamp":1686787200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Severance Hospital","award":["C-2021-0017"],"award-info":[{"award-number":["C-2021-0017"]}]},{"name":"Severance Hospital","award":["C-2022-0007"],"award-info":[{"award-number":["C-2022-0007"]}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Korean Government","award":["2022R1I1A1A01071723"],"award-info":[{"award-number":["2022R1I1A1A01071723"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,8,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>We evaluated the clinical accuracy and utility of whole-genome sequencing (WGS) of plasma microbial cell-free DNA (cfDNA) as a novel noninvasive method in diagnosing invasive aspergillosis (IA) in patients with hematologic malignancy (HM) or coronavirus disease 2019 (COVID-19).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Adults with HM or COVID-19 and suspected IA were recruited. IA cases were retrospectively diagnosed according to EORTC\/MSG definitions and ECMM\/ISHAM criteria for HM and COVID-19 patients, respectively. The results of cfDNA WGS were compared with the conventional diagnosis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Microbial cfDNA WGS was performed 53 times from 41 participants (19 from HM, 16 from COVID-19, and 7 from the control group). In participants with HM, Aspergillus cfDNA was detected in 100% of proven IA and 91.7% of probable IA cases. In participants with COVID-19, 50.0% of probable IA were positive for Aspergillus in cfDNA WGS. Concordance between Aspergillus cfDNA detection and proven\/probable IA conventional diagnosis was significantly higher in participants with HM than in those with COVID-19. IA diagnosed using EORTC\/MGS definitions showed significantly high concordance between Aspergillus cfDNA detection and proven\/probable IA.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Aspergillus cfDNA detection strongly correlated with proven\/probable IA diagnosed using EORTC\/MSG definitions and could be used as an additional diagnostic tool for IA.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiad213","type":"journal-article","created":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T04:14:47Z","timestamp":1686802487000},"page":"444-452","source":"Crossref","is-referenced-by-count":10,"title":["Utility of Plasma Microbial Cell-Free DNA Whole-Genome Sequencing for Diagnosis of Invasive Aspergillosis in Patients With Hematologic Malignancy or COVID-19"],"prefix":"10.1093","volume":"228","author":[{"given":"Ki Hyun","family":"Lee","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0084-0216","authenticated-orcid":false,"given":"Dongju","family":"Won","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinnam","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung Ah","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang Hyup","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung Ho","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su Jin","family":"Jeong","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nam Su","family":"Ku","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2775-3315","authenticated-orcid":false,"given":"Jun Yong","family":"Choi","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joon-Sup","family":"Yeom","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyunsoo","family":"Cho","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine, Yonsei Cancer Center, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haerim","family":"Chung","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine, Yonsei Cancer Center, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"June-Won","family":"Cheong","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine, Yonsei Cancer Center, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung-Tae","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji Eun","family":"Jang","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine, Yonsei Cancer Center, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1501-3923","authenticated-orcid":false,"given":"Saeam","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin Young","family":"Ahn","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine , Seoul , Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,6,15]]},"reference":[{"key":"2023081611073842800_jiad213-B1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.blre.2016.09.002","article-title":"Risk stratification for invasive fungal infections in patients with hematological malignancies: sEIFEM recommendations","volume":"31","author":"Pagano","year":"2017","journal-title":"Blood Rev"},{"key":"2023081611073842800_jiad213-B2","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/S2213-2600(21)00442-2","article-title":"Fungal infections in mechanically ventilated patients with COVID-19 during the first wave: the French multicentre MYCOVID study","volume":"10","author":"Gangneux","year":"2022","journal-title":"Lancet Respir Med"},{"key":"2023081611073842800_jiad213-B3","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1093\/cid\/ciz1008","article-title":"Revision and update of the consensus definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer and the Mycoses Study Group Education and Research Consortium","volume":"71","author":"Donnelly","year":"2020","journal-title":"Clin Infect Dis"},{"key":"2023081611073842800_jiad213-B4","doi-asserted-by":"crossref","DOI":"10.1016\/j.blre.2021.100906","article-title":"Diagnosis of infectious diseases in immunocompromised hosts using metagenomic next generation sequencing-based diagnostics","volume":"53","author":"Casto","year":"2022","journal-title":"Blood Rev"},{"key":"2023081611073842800_jiad213-B5","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1086\/429921","article-title":"Antifungal therapy decreases sensitivity of the Aspergillus galactomannan enzyme immunoassay","volume":"40","author":"Marr","year":"2005","journal-title":"Clin Infect Dis"},{"key":"2023081611073842800_jiad213-B6","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.pt.2016.01.006","article-title":"Cell-Free DNA as a diagnostic tool for human parasitic infections","volume":"32","author":"Weerakoon","year":"2016","journal-title":"Trends Parasitol"},{"key":"2023081611073842800_jiad213-B7","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1038\/s41564-018-0349-6","article-title":"Analytical and clinical validation of a microbial cell-free DNA sequencing test for infectious disease","volume":"4","author":"Blauwkamp","year":"2019","journal-title":"Nat Microbiol"},{"key":"2023081611073842800_jiad213-B8","first-page":"1937","article-title":"The discover trial: application of the Karius plasma next-generation sequencing test for pathogen detection in allogeneic stem-cell transplant patients","volume":"130","author":"Fung","year":"2017","journal-title":"Blood"},{"key":"2023081611073842800_jiad213-B9","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1093\/cid\/ciab158","article-title":"Clinical accuracy and impact of plasma cell-free DNA fungal polymerase chain reaction panel for noninvasive diagnosis of fungal infection","volume":"73","author":"Senchyna","year":"2021","journal-title":"Clin Infect Dis"},{"key":"2023081611073842800_jiad213-B10","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1093\/cid\/ciaa035","article-title":"Clinical impact of metagenomic next-generation sequencing of plasma cell-free DNA for the diagnosis of infectious diseases: a multicenter retrospective cohort study","volume":"72","author":"Hogan","year":"2021","journal-title":"Clin Infect Dis"},{"key":"2023081611073842800_jiad213-B11","doi-asserted-by":"crossref","first-page":"500.e1","DOI":"10.1016\/j.jtct.2021.02.025","article-title":"Impact of next-generation sequencing cell-free pathogen DNA test on antimicrobial management in adults with hematological malignancies and transplant recipients with suspected infections","volume":"27","author":"Yu","year":"2021","journal-title":"Transplant Cell Ther"},{"key":"2023081611073842800_jiad213-B12","doi-asserted-by":"crossref","DOI":"10.1093\/ofid\/ofz327","article-title":"Noninvasive diagnosis of infection using plasma next-generation sequencing: a single-center experience","volume":"6","author":"Rossoff","year":"2019","journal-title":"Open Forum Infect Dis"},{"key":"2023081611073842800_jiad213-B13","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.12688\/f1000research.19766.3","article-title":"Next-generation sequencing of microbial cell-free DNA for rapid noninvasive diagnosis of infectious diseases in immunocompromised hosts","volume":"8","author":"Camargo","year":"2019","journal-title":"F1000Res"},{"key":"2023081611073842800_jiad213-B14","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1093\/cid\/ciab324","article-title":"Plasma microbial cell-free DNA next-generation sequencing in the diagnosis and management of febrile neutropenia","volume":"74","author":"Benamu","year":"2022","journal-title":"Clin Infect Dis"},{"key":"2023081611073842800_jiad213-B15","doi-asserted-by":"crossref","first-page":"E3876","DOI":"10.1093\/cid\/ciaa1639","article-title":"Liquid biopsy for invasive mold infections in hematopoietic cell transplant recipients with pneumonia through next-generation sequencing of microbial cell-free DNA in plasma","volume":"73","author":"Hill","year":"2021","journal-title":"Clin Infect Dis"},{"key":"2023081611073842800_jiad213-B16","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.diagmicrobio.2018.06.009","article-title":"Liquid biopsy for infectious diseases: sequencing of cell-free plasma to detect pathogen DNA in patients with invasive fungal disease","volume":"92","author":"Hong","year":"2018","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2023081611073842800_jiad213-B17","doi-asserted-by":"crossref","DOI":"10.1093\/ofid\/ofy301","article-title":"Plasma cell-free DNA next-generation sequencing to diagnose and monitor infections in allogeneic hematopoietic stem cell transplant patients","volume":"5","author":"Fung","year":"2018","journal-title":"Open Forum Infect Dis"},{"key":"2023081611073842800_jiad213-B18","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1164\/rccm.201904-0905LE","article-title":"Detection of pneumonia pathogens from plasma cell-free DNA","volume":"201","author":"Langelier","year":"2020","journal-title":"Am J Respir Crit Care Med"},{"key":"2023081611073842800_jiad213-B19","doi-asserted-by":"crossref","first-page":"6903","DOI":"10.1038\/s41598-020-63970-0","article-title":"Cell free DNA from respiratory pathogens is detectable in the blood plasma of cystic fibrosis patients","volume":"10","author":"Barrett","year":"2020","journal-title":"Sci Rep"},{"key":"2023081611073842800_jiad213-B20","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1086\/588660","article-title":"Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer\/Invasive Fungal Infections Cooperative Group and the National Institute Of Allergy and Infectious Diseases Mycoses Study Group (EORTC\/MSG) consensus group","volume":"46","author":"De Pauw","year":"2008","journal-title":"Clin Infect Dis"},{"key":"2023081611073842800_jiad213-B21","doi-asserted-by":"crossref","first-page":"e149","DOI":"10.1016\/S1473-3099(20)30847-1","article-title":"Defining and managing COVID-19-associated pulmonary aspergillosis: the 2020 ECMM\/ISHAM consensus criteria for research and clinical guidance","volume":"21","author":"Koehler","year":"2021","journal-title":"Lancet Infect Dis"},{"key":"2023081611073842800_jiad213-B22","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"2023081611073842800_jiad213-B23","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat Methods"},{"key":"2023081611073842800_jiad213-B24","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1186\/s13059-019-1891-0","article-title":"Improved metagenomic analysis with kraken 2","volume":"20","author":"Wood","year":"2019","journal-title":"Genome Biol"},{"key":"2023081611073842800_jiad213-B25","doi-asserted-by":"crossref","DOI":"10.1128\/jcm.01859-22","article-title":"Metagenomic next-generation sequencing of plasma for diagnosis of COVID-19-associated pulmonary aspergillosis","volume":"61","author":"Hoenigl","year":"2023","journal-title":"J Clin Microbiol"},{"key":"2023081611073842800_jiad213-B26","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1111\/myc.13348","article-title":"Invasive aspergillosis due to Aspergillus cryptic species: a prospective multicentre study","volume":"64","author":"Imbert","year":"2021","journal-title":"Mycoses"},{"key":"2023081611073842800_jiad213-B27","article-title":"Polymerase chain reaction blood tests for the diagnosis of invasive aspergillosis in immunocompromised people","volume":"9","author":"Cruciani","year":"2019","journal-title":"Cochrane Database Syst Rev"},{"key":"2023081611073842800_jiad213-B28","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1136\/jcp.2011.090464","article-title":"Antifungal treatment affects the laboratory diagnosis of invasive aspergillosis","volume":"65","author":"McCulloch","year":"2012","journal-title":"J Clin Pathol"},{"key":"2023081611073842800_jiad213-B29","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.jmii.2020.09.004","article-title":"COVID-19 associated with pulmonary aspergillosis: a literature review","volume":"54","author":"Lai","year":"2021","journal-title":"J Microbiol Immunol Infect"}],"container-title":["The Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jid\/advance-article-pdf\/doi\/10.1093\/infdis\/jiad213\/50724305\/jiad213.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jid\/article-pdf\/228\/4\/444\/51115546\/jiad213.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jid\/article-pdf\/228\/4\/444\/51115546\/jiad213.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T11:08:03Z","timestamp":1692184083000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jid\/article\/228\/4\/444\/7198211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,15]]},"references-count":29,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,6,15]]},"published-print":{"date-parts":[[2023,8,16]]}},"URL":"https:\/\/doi.org\/10.1093\/infdis\/jiad213","relation":{},"ISSN":["0022-1899","1537-6613"],"issn-type":[{"value":"0022-1899","type":"print"},{"value":"1537-6613","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,8,15]]},"published":{"date-parts":[[2023,6,15]]}}}