{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T22:59:25Z","timestamp":1791932365850,"version":"4.3.4"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"Supplement_7","funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Ocular complications of Ebola virus disease are well-documented and long-term sequelae in survivors are common and lead to considerable morbidity. However, little is currently known regarding EBOV's tropism and replication kinetics within the eye. To date, limited studies have utilized in vitro infections of ocular cell lines and analyses of archived pathology samples to investigate these issues. Here, we employed ex vivo cultures of cynomolgus macaque eyes to determine the tropism of EBOV in 7 different ocular tissues: cornea, anterior sclera with bulbar conjunctiva, ciliary body, iris, lens, neural retina, and retina pigment epithelium. We report that, except for neural retina, all tissues supported EBOV replication. Retina pigment epithelium produced the fastest growth and highest viral RNA loads, although the differences were not statistically significant. Immunohistochemical staining confirmed and further characterized infection. This study demonstrates that EBOV has a broad tropism within the eye.<\/jats:p>","DOI":"10.1093\/infdis\/jiad239","type":"journal-article","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T03:43:35Z","timestamp":1688096615000},"page":"S626-S630","source":"Crossref","is-referenced-by-count":3,"title":["Ebola Virus Tropism in Ex Vivo Cynomolgus Macaque Ocular Tissues"],"prefix":"10.1093","volume":"228","author":[{"given":"M Jeremiah","family":"Matson","sequence":"first","affiliation":[{"name":"Laboratory of Virology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, Montana , USA"},{"name":"Department of Internal Medicine, University of Utah Health , Salt Lake City, Utah , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trent","family":"Bushmaker","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, Montana , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana P","family":"Scott","sequence":"additional","affiliation":[{"name":"Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Rosenke","sequence":"additional","affiliation":[{"name":"Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg","family":"Saturday","sequence":"additional","affiliation":[{"name":"Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel S","family":"Chertow","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, Montana , USA"},{"name":"Critical Care Medicine Department, Clinical Center, National Institutes of Health , Bethesda, Maryland , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2288-3196","authenticated-orcid":false,"given":"Vincent J","family":"Munster","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Hamilton, Montana , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,6,30]]},"reference":[{"key":"2023111521045654800_jiad239-B1","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.1056\/NEJMra1901594","article-title":"Ebola","volume":"382","author":"Feldmann","year":"2020","journal-title":"N Engl J Med"},{"issue":"Suppl 1","key":"2023111521045654800_jiad239-B2","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1086\/514308","article-title":"Ebola hemorrhagic fever in Kikwit, Democratic Republic of the Congo: clinical observations in 103 patients","volume":"179","author":"Bwaka","year":"1999","journal-title":"J Infect Dis"},{"issue":"Suppl 1","key":"2023111521045654800_jiad239-B3","doi-asserted-by":"crossref","first-page":"S13","DOI":"10.1086\/514288","article-title":"Late ophthalmologic manifestations in survivors of the 1995 Ebola virus epidemic in Kikwit, Democratic Republic of the 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