{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T10:42:47Z","timestamp":1776076967046,"version":"3.50.1"},"reference-count":8,"publisher":"Oxford University Press (OUP)","issue":"Supplement_2","license":[{"start":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T00:00:00Z","timestamp":1694131200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Cooperative Agreement","award":["NU60OE000104"],"award-info":[{"award-number":["NU60OE000104"]}]},{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008482","name":"Association of Public Health Laboratories","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008482","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012737","name":"Department of Health and Human Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012737","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This paper describes the methods for developing and optimizing a laboratory-developed assay (LDA) for detecting clade II human mpox virus using the automated Panther Fusion platform and Open Access software. Various concentrations of reagents in a primer-probe mix were tested to optimize the LDA. The LDA was validated using 10 previously characterized positive and 10 negative human mpox samples, resulting in 95% accuracy and 100% precision. The LDA resulted in 100% specificity among previously tested HSV1-, HSV2-, and VZV-positive human samples. Several spiked media extensions were also validated and achieved 98% accuracy and 100% precision across all collection media types. The assay's limit of detection was calculated to be 1.475 copies\/reaction, and the polymerase chain reaction efficiency resulted in 89.87% (slope, \u22123.5911; R2 = 0.9947). The methods described here can be applied to the rapid optimization and development of LDAs for many possible pathogens of public health importance.<\/jats:p>","DOI":"10.1093\/infdis\/jiad347","type":"journal-article","created":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T11:30:17Z","timestamp":1694172617000},"page":"S132-S136","source":"Crossref","is-referenced-by-count":4,"title":["A Laboratory-Developed Assay for Clade II Human Mpox Virus on the Panther Fusion Open Access System"],"prefix":"10.1093","volume":"229","author":[{"given":"Rebecca D","family":"Villa","sequence":"first","affiliation":[{"name":"State Hygienic Laboratory, University of Iowa , Coralville"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A","family":"Pentella","sequence":"additional","affiliation":[{"name":"State Hygienic Laboratory, University of Iowa , Coralville"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey L","family":"Benfer","sequence":"additional","affiliation":[{"name":"State Hygienic Laboratory, University of Iowa , Coralville"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,9,8]]},"reference":[{"key":"2024032619381305000_jiad347-B1","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1016\/S0196-0644(03)00819-9","article-title":"Update: multistate outbreak of monkeypox\u2014Illinois, Indiana, Kansas, Missouri, Ohio, and Wisconsin, 2003","volume":"42","author":"Gross","year":"2003","journal-title":"Ann Emerg Med"},{"key":"2024032619381305000_jiad347-B2","doi-asserted-by":"crossref","first-page":"68","DOI":"10.15585\/mmwr.mm7203a4","article-title":"Epidemiology of human mpox\u2014worldwide, 2018\u20132021","volume":"72","author":"Mccollum","year":"2023","journal-title":"Morb Mortal Wkly Rep"},{"key":"2024032619381305000_jiad347-B3","doi-asserted-by":"crossref","first-page":"2200422","DOI":"10.2807\/1560-7917.ES.2022.27.22.2200422","article-title":"Community transmission of monkeypox in the United Kingdom, April to May 2022","volume":"27","author":"Vivancos","year":"2022","journal-title":"Euro Surveill"},{"key":"2024032619381305000_jiad347-B4","author":"Centers for Disease Control and Prevention"},{"key":"2024032619381305000_jiad347-B5","doi-asserted-by":"crossref","first-page":"904","DOI":"10.15585\/mmwr.mm7128e1","article-title":"Rapid diagnostic testing for response to the monkeypox outbreak\u2014Laboratory Response Network, United States, May 17\u2013June 30, 2022","volume":"71","author":"Aden","year":"2022","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"2024032619381305000_jiad347-B6","author":"Centers for Disease Control and Prevention, Poxvirus and Rabies Branch"},{"key":"2024032619381305000_jiad347-B7","author":"US Department of Health and Human Services, Centers for Medicare Medicaid Services"},{"key":"2024032619381305000_jiad347-B8","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jviromet.2010.07.012","article-title":"Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA","volume":"169","author":"Li","year":"2010","journal-title":"J Virol Methods"}],"container-title":["The Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jid\/advance-article-pdf\/doi\/10.1093\/infdis\/jiad347\/51438430\/jiad347.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jid\/article-pdf\/229\/Supplement_2\/S132\/57094435\/jiad347.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jid\/article-pdf\/229\/Supplement_2\/S132\/57094435\/jiad347.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T20:06:08Z","timestamp":1711483568000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jid\/article\/229\/Supplement_2\/S132\/7263946"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,8]]},"references-count":8,"journal-issue":{"issue":"Supplement_2","published-online":{"date-parts":[[2023,9,8]]},"published-print":{"date-parts":[[2024,3,26]]}},"URL":"https:\/\/doi.org\/10.1093\/infdis\/jiad347","relation":{},"ISSN":["0022-1899","1537-6613"],"issn-type":[{"value":"0022-1899","type":"print"},{"value":"1537-6613","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,4,15]]},"published":{"date-parts":[[2023,9,8]]}}}