{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T23:43:52Z","timestamp":1782258232039,"version":"3.54.5"},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"Supplement_2","license":[{"start":{"date-parts":[[2023,9,22]],"date-time":"2023-09-22T00:00:00Z","timestamp":1695340800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100001316","name":"Abbott Laboratories","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001316","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The 2022 outbreak of the clade IIb monkeypox virus and subsequent global spread lead to an urgent need for the development of high-throughput, sensitive, and reproducible diagnostic tests.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We developed 3 assays to detect monkeypox virus, 2 (MPXV+ and MPXV) for m2000 RealTime and 1 (MPXV) for Alinity m platforms. Dual targets in E9L and B6R (MPXV+) and J2L and B7R (MPXV) increased mutation resistance. In silico prediction indicates MPXV+ cross-reactivity with orthopox viruses and specific monkeypox virus detection with MPXV.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>m2000 RealTime MPXV+ and MPXV assay sensitivity was determined to be 3.2 plaque-forming units\/mL using a reference virus culture diluted into universal transport medium (UTM). Alinity m MPXV lower limit of detection was 200\u2005copies\/mL using monkeypox virus plasmids in pooled UTM matrix. m2000 RealTime MPXV+ and MPXV assays were validated with lesion swabs in UTM and 1:1 saliva to UTM mixtures. Commercially available and remnant clinical lesion specimens in UTM were tested with RealTime MPXV+, RealTime MPXV and Alinity m MPXV assays and demonstrated high agreement to known mpox (MPX)-positive specimens.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>RealTime MPXV+, RealTime MPXV, and Alinity MPXV are high throughput and sensitive assays used for the detection of monkeypox virus. These assays maybe useful during MPX outbreaks.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiad406","type":"journal-article","created":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T01:18:16Z","timestamp":1695431896000},"page":"S137-S143","source":"Crossref","is-referenced-by-count":9,"title":["Development and Validation of Three Automated High-Throughput Molecular Tests to Detect Monkeypox Virus Infections"],"prefix":"10.1093","volume":"229","author":[{"given":"Mark","family":"Anderson","sequence":"first","affiliation":[{"name":"Infectious Disease Research, Abbott Diagnostics Division , Abbott Park, Illinois , USA"},{"name":"Abbott Pandemic Defense Coalition , Abbott Park, Illinois , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austin","family":"Hodges","sequence":"additional","affiliation":[{"name":"Infectious Disease Research, Abbott Diagnostics Division , Abbott Park, Illinois , USA"},{"name":"Abbott Pandemic Defense Coalition , Abbott Park, Illinois , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ka-Cheung","family":"Luk","sequence":"additional","affiliation":[{"name":"Infectious Disease Research, Abbott Diagnostics Division , Abbott Park, Illinois , USA"},{"name":"Abbott Pandemic Defense Coalition , Abbott Park, Illinois , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Olivo","sequence":"additional","affiliation":[{"name":"Infectious Disease Research, Abbott Diagnostics Division , Abbott Park, Illinois , USA"},{"name":"Abbott Pandemic Defense Coalition , Abbott Park, Illinois , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenn","family":"Forberg","sequence":"additional","affiliation":[{"name":"Infectious Disease Research, Abbott Diagnostics Division , Abbott Park, Illinois , USA"},{"name":"Abbott Pandemic Defense Coalition , Abbott Park, Illinois , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd V","family":"Meyer","sequence":"additional","affiliation":[{"name":"Infectious Disease Research, Abbott Diagnostics Division , Abbott Park, Illinois , USA"},{"name":"Abbott Pandemic Defense Coalition , Abbott Park, Illinois , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn","family":"Strobel","sequence":"additional","affiliation":[{"name":"Infectious Disease Research, Abbott Diagnostics Division , Abbott Park, Illinois , USA"},{"name":"Abbott Pandemic Defense Coalition , Abbott Park, Illinois , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Kim","sequence":"additional","affiliation":[{"name":"Abbott Molecular Diagnostics , Des Plaines, Illinois , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Toolsie","sequence":"additional","affiliation":[{"name":"Abbott Molecular Diagnostics , Des Plaines, Illinois , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9734-8905","authenticated-orcid":false,"given":"Nicholas M","family":"Moore","sequence":"additional","affiliation":[{"name":"Rush University Medical Center , Chicago, Illinois , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yitzchak","family":"Goldstein","sequence":"additional","affiliation":[{"name":"Montefiore Medical Center , Bronx, NewYork , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Rodgers","sequence":"additional","affiliation":[{"name":"Infectious Disease Research, Abbott Diagnostics Division , Abbott Park, Illinois , USA"},{"name":"Abbott Pandemic Defense Coalition , Abbott Park, Illinois , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danijela","family":"Lucic","sequence":"additional","affiliation":[{"name":"Abbott Molecular Diagnostics , Des Plaines, Illinois , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gavin","family":"Cloherty","sequence":"additional","affiliation":[{"name":"Infectious Disease Research, Abbott Diagnostics Division , Abbott Park, Illinois , USA"},{"name":"Abbott Pandemic Defense Coalition , Abbott Park, Illinois , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,9,22]]},"reference":[{"key":"2024032619381721000_jiad406-B1","author":"World Health Organization"},{"key":"2024032619381721000_jiad406-B2","doi-asserted-by":"crossref","first-page":"e0010141","DOI":"10.1371\/journal.pntd.0010141","article-title":"The changing epidemiology of human monkeypox\u2014a potential threat? 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