{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T04:23:44Z","timestamp":1771302224160,"version":"3.50.1"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"Supplement_2","license":[{"start":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T00:00:00Z","timestamp":1711411200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>In 2022, the global dissemination of mpox virus (MPXV) outside endemic regions prompted the expansion of diagnostic testing worldwide. This study assesses the performance characteristics of 5 real-time polymerase chain reaction (PCR) assays in detecting MPXV during the 2022 outbreak.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Clinical specimens collected from patients across Ontario, Canada, were tested on the following assays: RealStar Orthopoxyvirus PCR and FlexStar Monkeypox virus PCR (Altona Diagnostics), Novaplex MPXV (Seegene), VIASURE Monkeypox virus Real Time PCR Reagents (CerTest Biotec), and a laboratory-developed test. Positive percent agreement (PPA), negative percent agreement (NPA), relative limit of detection (LOD), and precision were evaluated and MPXV lineages were determined using an amplicon-based whole-genome sequencing (WGS) assay.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Swabs were collected from various anatomic sites (65 positive and 30 negative). All assays demonstrated 100% NPA (95% confidence interval, 88.4%\/88.1%\u2013100.0%), with PPA ranging from 92.2% (82.7%\u201397.4%) to 96.9% (89.3%\u201399.6%). LOD and precision were comparable across assays, with coefficient of variations &amp;lt;3%. WGS analysis identified 6 lineages, all belonging to subclade IIb.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The assays exhibited excellent PPA, NPA, LOD, and precision. Ongoing performance monitoring is essential to detect assay escape mutants and ensure universal detection of evolving MPXV strains.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiad464","type":"journal-article","created":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T20:05:46Z","timestamp":1711483546000},"page":"S156-S162","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of 5 Polymerase Chain Reaction Assays for the Detection of Mpox Virus"],"prefix":"10.1093","volume":"229","author":[{"given":"Ramzi","family":"Fattouh","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, St Michael's Hospital, Unity Health Toronto , Toronto , Canada"},{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto , Toronto , Canada"},{"name":"Li Ka Shing Knowledge Institute, Unity Health Toronto , Toronto , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karel","family":"Boissinot","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, St Michael's Hospital, Unity Health Toronto , Toronto , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, St Michael's Hospital, Unity Health Toronto , Toronto , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew B","family":"Mendlowitz","sequence":"additional","affiliation":[{"name":"Toronto Centre for Liver Disease\/Viral Hepatitis Care Network (VIRCAN), University Health Network , Toronto , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Calvin P","family":"Sjaarda","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Kingston Health Sciences Centre , Kingston , Canada"},{"name":"Department of Pathology and Molecular Medicine, Queen\u2019s University , Kingston , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry","family":"Wong","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Kingston Health Sciences Centre , Kingston , Canada"},{"name":"Department of Pathology and Molecular Medicine, Queen\u2019s University , Kingston , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Kozak","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto , Toronto , Canada"},{"name":"Sunnybrook Health Sciences Centre , Toronto , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prameet M","family":"Sheth","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Kingston Health Sciences Centre , Kingston , Canada"},{"name":"Department of Pathology and Molecular Medicine, Queen\u2019s University , Kingston , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7944-1348","authenticated-orcid":false,"given":"Larissa M","family":"Matukas","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, St Michael's Hospital, Unity Health Toronto , Toronto , Canada"},{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto , Toronto , Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,3,26]]},"reference":[{"key":"2024032619342351000_jiad464-B1","doi-asserted-by":"crossref","first-page":"198932","DOI":"10.1016\/j.virusres.2022.198932","article-title":"The virology of human monkeypox virus (hMPXV): a brief overview","volume":"322","author":"Lansiaux","year":"2022","journal-title":"Virus Res"},{"key":"2024032619342351000_jiad464-B2","author":"World Health Organization"},{"key":"2024032619342351000_jiad464-B3","author":"World Health Organization"},{"key":"2024032619342351000_jiad464-B4","doi-asserted-by":"crossref","first-page":"e0010141","DOI":"10.1371\/journal.pntd.0010141","article-title":"The changing epidemiology of human monkeypox\u2014a potential threat? 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