{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T23:12:31Z","timestamp":1787785951704,"version":"build-2784847793"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T00:00:00Z","timestamp":1701302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill & Melinda Gates Foundation","doi-asserted-by":"publisher","award":["INV-008522"],"award-info":[{"award-number":["INV-008522"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The key correlate of protection of respiratory syncytial virus (RSV) vaccines and monoclonal antibodies (mAbs) is virus neutralization, measured via sera obtained through venipuncture. Dried blood obtained with a finger prick can simplify acquisition, processing, storage, and transport in trials and thereby reduce costs. In this study, we validate an assay to measure RSV neutralization in dried capillary blood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Functional antibodies were compared between matched serum and dried blood samples from a phase 1 trial with RSM01, an investigational anti-RSV prefusion F mAb. Hep-2 cells were infected with a serial dilution of sample-virus mixture by using RSV-A2-mKate to determine the half-maximal inhibitory concentration. Stability of dried blood was evaluated over time and during temperature stress.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Functional antibodies in dried blood were highly correlated with serum (R2 = 0.98, P &amp;lt; .0001). The precision of the assay for dried blood was similar to serum. The function of mAb remained stable for 9 months at room temperature and frozen dried blood samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We demonstrated the feasibility of measuring RSV neutralization using dried blood as a patient-centered solution that may replace serology testing in trials against RSV or other viruses, such as influenza and SARS-CoV-2.<\/jats:p>\n                    <jats:p>Clinical Trials Registration. \u2003NCT05118386 (ClinicalTrials.gov).<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/infdis\/jiad543","type":"journal-article","created":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T18:06:52Z","timestamp":1701194812000},"page":"e93-e101","source":"Crossref","is-referenced-by-count":8,"title":["RSV Neutralizing Antibodies in Dried Blood"],"prefix":"10.1093","volume":"230","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2986-0138","authenticated-orcid":false,"given":"Jonne","family":"Terstappen","sequence":"first","affiliation":[{"name":"Department of Pediatric Infectious Diseases and Immunology, Wilhelmina Children's Hospital, University Medical Center Utrecht, the Netherlands"},{"name":"Center for Translational Immunology, University Medical Centre Utrecht , the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eveline M","family":"Delemarre","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, University Medical Centre Utrecht , the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anouk","family":"Versnel","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, University Medical Centre Utrecht , the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joleen T","family":"White","sequence":"additional","affiliation":[{"name":"Bioassay Development and Operations, Bill & Melinda Gates Medical Research Institute , Cambridge, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandrine","family":"Derrien-Colemyn","sequence":"additional","affiliation":[{"name":"Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy J","family":"Ruckwardt","sequence":"additional","affiliation":[{"name":"Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3099-4475","authenticated-orcid":false,"given":"Louis J","family":"Bont","sequence":"additional","affiliation":[{"name":"Department of Pediatric Infectious Diseases and Immunology, Wilhelmina Children's Hospital, University Medical Center Utrecht, the Netherlands"},{"name":"Center for Translational Immunology, University Medical Centre Utrecht , the Netherlands"},{"name":"Respiratory Syncytial Virus Network Foundation , Zeist"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie I","family":"Mazur","sequence":"additional","affiliation":[{"name":"Department of Pediatric Infectious Diseases and Immunology, Wilhelmina Children's Hospital, University Medical Center Utrecht, the Netherlands"},{"name":"Center for Translational Immunology, University Medical Centre Utrecht , the Netherlands"},{"name":"Department of Pediatrics, St Antonius Hospital , Nieuwegein , Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,11,30]]},"reference":[{"key":"2024072520120900600_jiad543-B1","doi-asserted-by":"crossref","first-page":"e2","DOI":"10.1016\/S1473-3099(22)00291-2","article-title":"Respiratory syncytial virus prevention within reach: the vaccine and monoclonal antibody landscape","volume":"23","author":"Mazur","year":"2023","journal-title":"Lancet Infect Dis"},{"key":"2024072520120900600_jiad543-B2","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1056\/NEJMoa1913556","article-title":"Single-dose nirsevimab for prevention of RSV in preterm infants","volume":"383","author":"Griffin","year":"2020","journal-title":"N Engl J Med"},{"key":"2024072520120900600_jiad543-B3","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1056\/NEJMoa2110275","article-title":"Nirsevimab for prevention of RSV in healthy late-preterm and term infants","volume":"386","author":"Hammitt","year":"2022","journal-title":"N Engl J Med"},{"key":"2024072520120900600_jiad543-B4","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.1016\/S0140-6736(22)00478-0","article-title":"Global, regional, and national disease burden estimates of acute lower respiratory infections due to respiratory syncytial virus in children younger than 5 years in 2019: a systematic analysis","volume":"399","author":"Li","year":"2022","journal-title":"Lancet"},{"key":"2024072520120900600_jiad543-B5","doi-asserted-by":"crossref","first-page":"961","DOI":"10.3390\/vaccines9090961","article-title":"Bringing preventive RSV monoclonal antibodies to infants in low- and middle-income countries: challenges and opportunities","volume":"9","author":"Ananworanich","year":"2021","journal-title":"Vaccines"},{"key":"2024072520120900600_jiad543-B6","doi-asserted-by":"crossref","first-page":"4591","DOI":"10.1016\/j.vaccine.2021.06.016","article-title":"RSV neutralization assays\u2014use in immune response assessment","volume":"39","author":"Raghunandan","year":"2021","journal-title":"Vaccine"},{"key":"2024072520120900600_jiad543-B7","doi-asserted-by":"crossref","first-page":"815","DOI":"10.4155\/bio-2018-0010","article-title":"Capillary blood microsampling to determine serum biopharmaceutical concentration: Mitra microsampler vs dried blood spot","volume":"10","author":"Bloem","year":"2018","journal-title":"Bioanalysis"},{"key":"2024072520120900600_jiad543-B8","doi-asserted-by":"crossref","first-page":"e0129821","DOI":"10.1128\/Spectrum.01298-21","article-title":"SARS-CoV-2 antibody binding and neutralization in dried blood spot eluates and paired plasma","volume":"9","author":"Itell","year":"2021","journal-title":"Microbiol Spectr"},{"key":"2024072520120900600_jiad543-B9","doi-asserted-by":"crossref","first-page":"e00043-18","DOI":"10.1128\/mSphere.00043-18","article-title":"Evaluation of dried blood spots and oral fluids as alternatives to serum for human papillomavirus antibody surveillance","volume":"3","author":"Louie","year":"2018","journal-title":"mSphere"},{"key":"2024072520120900600_jiad543-B10","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.4155\/bio-2021-0065","article-title":"Validation of high-throughput, semiquantitative solid-phase SARS coronavirus-2 serology assays in serum and dried blood spot matrices","volume":"13","author":"Maritz","year":"2021","journal-title":"Bioanalysis"},{"key":"2024072520120900600_jiad543-B11","doi-asserted-by":"crossref","first-page":"15321","DOI":"10.1038\/s41598-021-94653-z","article-title":"A surrogate virus neutralization test to quantify antibody-mediated inhibition of SARS-CoV-2 in finger stick dried blood spot samples","volume":"11","author":"Sancilio","year":"2021","journal-title":"Sci Rep"},{"key":"2024072520120900600_jiad543-B12","doi-asserted-by":"crossref","first-page":"e0008784","DOI":"10.1371\/journal.pntd.0008784","article-title":"Dried blood spot cards: a reliable sampling method to detect human antibodies against rabies virus","volume":"14","author":"Doornekamp","year":"2020","journal-title":"PLoS Negl Trop Dis"},{"key":"2024072520120900600_jiad543-B13","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.aca.2018.09.004","article-title":"Tutorial: volumetric absorptive microsampling (VAMS)","volume":"1046","author":"Protti","year":"2019","journal-title":"Anal Chim Acta"},{"key":"2024072520120900600_jiad543-B14","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1038\/s41467-020-20383-x","article-title":"Standardization of ELISA protocols for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling","volume":"12","author":"Klumpp-Thomas","year":"2021","journal-title":"Nat Commun"},{"key":"2024072520120900600_jiad543-B15","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3390\/vaccines7010023","article-title":"Epitope-specific serological assays for RSV: conformation matters","volume":"7","author":"Phung","year":"2019","journal-title":"Vaccines (Basel)"},{"key":"2024072520120900600_jiad543-B16","doi-asserted-by":"crossref","first-page":"309ra162","DOI":"10.1126\/scitranslmed.aac4241","article-title":"Prefusion F-specific antibodies determine the magnitude of RSV neutralizing activity in human sera","volume":"7","author":"Ngwuta","year":"2015","journal-title":"Sci Transl Med"},{"key":"2024072520120900600_jiad543-B17","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.virol.2012.09.022","article-title":"A stabilized respiratory syncytial virus reverse genetics system amenable to recombination-mediated mutagenesis","volume":"434","author":"Hotard","year":"2012","journal-title":"Virology"},{"key":"2024072520120900600_jiad543-B18","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/978-1-4939-6964-7_8","article-title":"BAC-based recovery of recombinant respiratory syncytial virus (RSV)","volume":"1602","author":"Stobart","year":"2017","journal-title":"Methods Mol Biol"},{"key":"2024072520120900600_jiad543-B19","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1208\/s12248-014-9573-z","article-title":"Stability: recommendation for best practices and harmonization from the global bioanalysis consortium harmonization team","volume":"16","author":"van de Merbel","year":"2014","journal-title":"AAPS J"},{"key":"2024072520120900600_jiad543-B20","doi-asserted-by":"crossref","first-page":"e1261","DOI":"10.1002\/cti2.1261","article-title":"Comprehensive analysis of SARS-CoV-2 antibody dynamics in New Zealand","volume":"10","author":"Whitcombe","year":"2021","journal-title":"Clin Transl Immunol"},{"key":"2024072520120900600_jiad543-B21","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.jpba.2018.10.010","article-title":"Is the hematocrit still an issue in quantitative dried blood spot analysis?","volume":"163","author":"Velghe","year":"2019","journal-title":"J Pharm Biomed Anal"},{"key":"2024072520120900600_jiad543-B22","doi-asserted-by":"crossref","first-page":"e0248218","DOI":"10.1371\/journal.pone.0248218","article-title":"Reliability of dried blood spot (DBS) cards in antibody measurement: a systematic review","volume":"16","author":"Amini","year":"2021","journal-title":"PLoS One"},{"key":"2024072520120900600_jiad543-B23","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1590\/S0036-46652008000300004","article-title":"Evaluation of the human immunodeficiency virus type 1 and 2 antibodies detection in dried whole blood spots (DBS) samples","volume":"50","author":"Castro","year":"2008","journal-title":"Rev Inst Med Trop Sao Paulo"},{"key":"2024072520120900600_jiad543-B24","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.xphs.2019.08.009","article-title":"Physicochemical stability of monoclonal antibodies: a review","volume":"109","author":"Le Basle","year":"2020","journal-title":"J Pharm Sci"},{"key":"2024072520120900600_jiad543-B25","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.biochi.2020.08.019","article-title":"Antibody stability: a key to performance\u2014analysis, influences and improvement","volume":"177","author":"Ma","year":"2020","journal-title":"Biochimie"},{"key":"2024072520120900600_jiad543-B26","doi-asserted-by":"crossref","first-page":"e0049021","DOI":"10.1128\/mSphere.00490-21","article-title":"Optimization and stability testing of four commercially available dried blood spot devices for estimating measles and rubella IgG antibodies","volume":"6","author":"Kaduskar","year":"2021","journal-title":"mSphere"},{"key":"2024072520120900600_jiad543-B27","volume-title":"Clinical methods: the history, physical, and laboratory examinations","author":"Billett","year":"1990","edition":"3rd ed"},{"key":"2024072520120900600_jiad543-B28","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1056\/NEJMoa1908380","article-title":"Respiratory syncytial virus vaccination during pregnancy and effects in infants","volume":"383","author":"Madhi","year":"2020","journal-title":"N Engl J Med"}],"container-title":["The Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jid\/advance-article-pdf\/doi\/10.1093\/infdis\/jiad543\/54903568\/jiad543.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jid\/article-pdf\/230\/1\/e93\/58649438\/jiad543.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jid\/article-pdf\/230\/1\/e93\/58649438\/jiad543.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T16:15:58Z","timestamp":1721924158000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jid\/article\/230\/1\/e93\/7456299"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,30]]},"references-count":28,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,11,30]]},"published-print":{"date-parts":[[2024,7,25]]}},"URL":"https:\/\/doi.org\/10.1093\/infdis\/jiad543","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.08.10.23293875","asserted-by":"object"}]},"ISSN":["0022-1899","1537-6613"],"issn-type":[{"value":"0022-1899","type":"print"},{"value":"1537-6613","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,7,15]]},"published":{"date-parts":[[2023,11,30]]}}}