{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T02:01:14Z","timestamp":1793152874411,"version":"4.5.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T00:00:00Z","timestamp":1703548800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82272383"],"award-info":[{"award-number":["82272383"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070189"],"award-info":[{"award-number":["32070189"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31900160"],"award-info":[{"award-number":["31900160"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2021JJ30594"],"award-info":[{"award-number":["2021JJ30594"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2019JJ50554"],"award-info":[{"award-number":["2019JJ50554"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2023JJ30540"],"award-info":[{"award-number":["2023JJ30540"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["201RHX002"],"award-info":[{"award-number":["201RHX002"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Research Program of Health Commission of Hunan Province","award":["C202304127239"],"award-info":[{"award-number":["C202304127239"]}]},{"DOI":"10.13039\/501100009020","name":"University of South China","doi-asserted-by":"publisher","award":["20224310NHYCG02"],"award-info":[{"award-number":["20224310NHYCG02"]}],"id":[{"id":"10.13039\/501100009020","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The pathogenesis of Chlamydia trachomatis is associated with the induction of the host inflammatory response; however, the precise underlying molecular mechanisms remain poorly understood.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>CT622, a T3SS effector protein, has an important role in the pathogenesis of C trachomatis; however, whether CT622 can induce a host inflammatory response is not understood. Our findings demonstrate that CT622 induces the expression of interleukins 6 and 8 (IL-6 and IL-8). Mechanistically, these effects involve the activation of the MAPK\/NF-\u03baB signaling pathways (mitogen-activated protein kinase\/nuclear factor \u03baB).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Interestingly, we demonstrated that the suppression of toll-like receptor 4 using small interfering RNA markedly reduced the phosphorylation of ERK, p38, JNK, and I\u03baB\u03b1, concomitant with a significant decrease in IL-6 and IL-8 secretion. Conversely, disruption of toll-like receptor 2 abrogated the CT622-induced upregulation of IL-8 and activation of ERK, whereas IL-6 expression and p38, JNK, and I\u03baB\u03b1 phosphorylation were unaffected.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Taken together, these results indicate that CT622 contributes to the inflammatory response through the toll-like receptor 2\/4\u2013mediated MAPK\/NF-\u03baB pathways, which provides insight into the molecular pathology of C trachomatis infection.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiad597","type":"journal-article","created":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T00:52:03Z","timestamp":1703379123000},"page":"1637-1647","source":"Crossref","is-referenced-by-count":13,"title":["<i>Chlamydia trachomatis<\/i> T3SS Effector CT622 Induces Proinflammatory Cytokines Through TLR2\/TLR4-Mediated MAPK\/NF-\u03baB Pathways in THP-1 Cells"],"prefix":"10.1093","volume":"229","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7523-355X","authenticated-orcid":false,"given":"Wenbo","family":"Lei","sequence":"first","affiliation":[{"name":"School of Nursing, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, Institute of Pathogenic Biology"},{"name":"Department of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China , Hengyang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yating","family":"Wen","sequence":"additional","affiliation":[{"name":"School of Nursing, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, Institute of Pathogenic Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yewei","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Nursing, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, Institute of Pathogenic Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangquan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China , Hengyang , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyu","family":"Li","sequence":"additional","affiliation":[{"name":"School of Nursing, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, Institute of Pathogenic Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,12,26]]},"reference":[{"key":"2024061323522941800_jiad597-B1","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.3390\/antibiotics11111634","article-title":"Therapeutic options for Chlamydia trachomatis infection: present and future","volume":"11","author":"Rodrigues","year":"2022","journal-title":"Antibiotics (Basel)"},{"key":"2024061323522941800_jiad597-B2","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.tim.2022.09.002","article-title":"Regulation of chlamydial colonization by IFNgamma delivered via distinct cells","volume":"31","author":"Winner","year":"2023","journal-title":"Trends Microbiol"},{"key":"2024061323522941800_jiad597-B3","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.3389\/fmicb.2019.01412","article-title":"Clear victory for Chlamydia: the subversion of host innate immunity","volume":"10","author":"Chen","year":"2019","journal-title":"Front Microbiol"},{"key":"2024061323522941800_jiad597-B4","doi-asserted-by":"crossref","first-page":"ftaa078","DOI":"10.1093\/femspd\/ftaa078","article-title":"Got mutants? 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