{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:00:46Z","timestamp":1791327646051,"version":"4.3.1"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T00:00:00Z","timestamp":1708905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012553","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CMRPG6J0231-0233"],"award-info":[{"award-number":["CMRPG6J0231-0233"]}],"id":[{"id":"10.13039\/100012553","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012553","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CMRPG6L0441-0443"],"award-info":[{"award-number":["CMRPG6L0441-0443"]}],"id":[{"id":"10.13039\/100012553","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012553","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CORPG6M0051"],"award-info":[{"award-number":["CORPG6M0051"]}],"id":[{"id":"10.13039\/100012553","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012553","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CORPG6P0011"],"award-info":[{"award-number":["CORPG6P0011"]}],"id":[{"id":"10.13039\/100012553","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["109-2314-B-182A-024-MY3"],"award-info":[{"award-number":["109-2314-B-182A-024-MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NMRPG6K6011-13"],"award-info":[{"award-number":["NMRPG6K6011-13"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Methicillin-resistant Staphylococcus aureus (MRSA) sequence type (ST) 45 is a globally disseminated MRSA lineage. Herein, we investigated whether MRSA ST45 isolates from cellulitis and from osteomyelitis display distinctive phenotypic and genomic characteristics.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A total of 15 MRSA ST45 isolates from cellulitis (CL-MRSA; n = 6) or osteomyelitis (OM-MRSA; n = 9) were collected in a Taiwan hospital. These MRSA ST45 isolates were characterized for their antimicrobial susceptibility, biofilm-forming ability, cellular infectivity in vitro, and pathogenicity in vivo. Four CL-MRSA and 6 OM-MRSA ST45 isolates were selected for whole-genome sequencing (WGS).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Antibiotic resistance tests showed that all OM-MRSA ST45 strains, but not CL-MRSA ST45 strains, were resistant to ciprofloxacin, levofloxacin, gentamicin, and doxycycline. Compared to the CL-MRSA ST45 isolates, the OM-MRSA ST45 isolates had stronger biofilm-forming ability and cellular infectivity and caused more severe disease in mice. WGS analysis revealed that these OM-MRSA ST45 isolates carry multiple common mutations or polymorphisms in genes associated with antibiotic resistance and virulence. Moreover, the transposable elements IS256 and IS257R2 were found only in the OM-MRSA ST45 isolates.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The emergence and spread of the highly pathogenic and multidrug-resistant ST45 MRSAs identified from osteomyelitis may pose a serious threat on public health.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiae096","type":"journal-article","created":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T04:16:18Z","timestamp":1709007378000},"page":"e568-e578","source":"Crossref","is-referenced-by-count":7,"title":["A Comparative Phenotypic and Genomic Analysis of Methicillin-Resistant <i>Staphylococcus aureus<\/i> ST45 Isolates From Cellulitis and Osteomyelitis in Taiwan"],"prefix":"10.1093","volume":"230","author":[{"given":"Kuo-Ti","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery, Chang Gung Memorial Hospital , Chiayi"},{"name":"College of Medicine, Chang Gung University , Taoyuan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Chun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Chang Gung Memorial Hospital , Chiayi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiun-Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Chang Gung Memorial Hospital , Chiayi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsung-Yu","family":"Huang","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, Chang Gung Memorial Hospital , Chiayi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ho","family":"Peng","sequence":"additional","affiliation":[{"name":"National Chia-Yi Girls Senior High School , Chiayi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju-Fang","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Oral Hygiene, College of Oral Medicine, Taipei Medical University , Taipei"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiang-Wen","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Nursing, Division of Basic Medical Sciences, Recurrent Diseases and Health Promotion Research Center, Chang Gung University of Science and Technology , Chiayi"},{"name":"Research Center for Industry of Human Ecology and Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology , Taoyuan"},{"name":"Department of Rehabilitation, Chang Gung Memorial Hospital , Chiayi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6492-0346","authenticated-orcid":false,"given":"Pey-Jium","family":"Chang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University , Taoyuan"},{"name":"Department of Nephrology, Chang Gung Memorial Hospital , Chiayi , Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,2,26]]},"reference":[{"key":"2024092316135137000_jiae096-B1","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/S0966-842X(01)02175-8","article-title":"The emergence and evolution of methicillin-resistant Staphylococcus aureus","volume":"9","author":"Hiramatsu","year":"2001","journal-title":"Trends 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