{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T09:06:56Z","timestamp":1740128816119,"version":"3.37.3"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T00:00:00Z","timestamp":1708905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01","AI127544"],"award-info":[{"award-number":["R01","AI127544"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007108","name":"George Washington University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Plasmodium falciparum and Plasmodium vivax account for &amp;gt;90% global malaria burden. Transmission intervention strategies encompassing transmission-blocking vaccines (TBV) and drugs represent ideal public health tools to eliminate malaria at the population level. The availability of mature P. falciparum gametocytes through in vitro culture has facilitated development of a standard membrane feeding assay to assess efficacy of transmission interventions against P. falciparum. The lack of in vitro culture for P. vivax has significantly hampered similar progress on P. vivax and limited studies have been possible using blood from infected patients in endemic areas. The ethical and logistical limitations of on-time access to blood from patients have impeded the development of P. vivax TBVs.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Transgenic murine malaria parasites (Plasmodium berghei) expressing TBV candidates offer a promising alternative for evaluation of P. vivax TBVs through in vivo studies in mice, and ex vivo membrane feeding assay (MFA).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We describe the development of transmission-competent transgenic TgPbvs25 parasites and optimization of parameters to establish an ex vivo MFA to evaluate P. vivax TBV based on Pvs25 antigen.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The MFA is expected to expedite Pvs25-based TBV development without dependence on blood from P. vivax-infected patients in endemic areas for evaluation.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiae102","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T22:01:25Z","timestamp":1708984885000},"page":"1894-1903","source":"Crossref","is-referenced-by-count":1,"title":["A Novel <i>Ex Vivo<\/i> Assay to Evaluate Functional Effectiveness of <i>Plasmodium vivax<\/i> Transmission-Blocking Vaccine Using Pvs25 Transgenic <i>Plasmodium berghei<\/i>"],"prefix":"10.1093","volume":"229","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8780-1350","authenticated-orcid":false,"given":"Yi","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Global Health, Milken Institute School of Public Health, George Washington University , Washington, District of Columbia , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clifford T H","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Global Health, Milken Institute School of Public Health, George Washington University , Washington, District of Columbia , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0358-4924","authenticated-orcid":false,"given":"Nirbhay","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Global Health, Milken Institute School of Public Health, George Washington University , Washington, District of Columbia , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,2,26]]},"reference":[{"volume-title":"World malaria report 2023","year":"2023","author":"World Health Organization","key":"2024061323534067300_jiae102-B1"},{"key":"2024061323534067300_jiae102-B2","doi-asserted-by":"crossref","first-page":"e156588","DOI":"10.1172\/JCI156588","article-title":"RTS, S: the first malaria vaccine","volume":"132","author":"Zavala","year":"2022","journal-title":"J Clin Invest"},{"key":"2024061323534067300_jiae102-B3","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.3390\/vaccines10071134","article-title":"Effective functional immunogenicity of a DNA vaccine combination delivered via in vivo electroporation targeting malaria infection and transmission","volume":"10","author":"Cao","year":"2022","journal-title":"Vaccines (Basel)"},{"key":"2024061323534067300_jiae102-B4","doi-asserted-by":"crossref","first-page":"3205","DOI":"10.1016\/j.vaccine.2003.11.060","article-title":"Potent immunogenicity of DNA vaccines encoding Plasmodium vivax transmission-blocking vaccine candidates Pvs25 and Pvs28-evaluation of homologous and heterologous antigen-delivery prime-boost strategy","volume":"22","author":"Kongkasuriyachai","year":"2004","journal-title":"Vaccine"},{"key":"2024061323534067300_jiae102-B5","doi-asserted-by":"crossref","first-page":"6618","DOI":"10.1128\/IAI.68.12.6618-6623.2000","article-title":"Antibodies to malaria vaccine candidates Pvs25 and Pvs28 completely block the ability of Plasmodium vivax to infect mosquitoes","volume":"68","author":"Hisaeda","year":"2000","journal-title":"Infect Immun"},{"key":"2024061323534067300_jiae102-B6","doi-asserted-by":"crossref","first-page":"e2636","DOI":"10.1371\/journal.pone.0002636","article-title":"Phase 1 trial of malaria transmission blocking vaccine candidates Pfs25 and Pvs25 formulated with montanide ISA 51","volume":"3","author":"Wu","year":"2008","journal-title":"PLoS One"},{"key":"2024061323534067300_jiae102-B7","doi-asserted-by":"crossref","first-page":"3131","DOI":"10.1016\/j.vaccine.2004.12.019","article-title":"Phase 1 vaccine trial of Pvs25H: a transmission blocking vaccine for Plasmodium vivax malaria","volume":"23","author":"Malkin","year":"2005","journal-title":"Vaccine"},{"key":"2024061323534067300_jiae102-B8","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1016\/j.vaccine.2012.01.003","article-title":"Plasmodium vivax gametocyte protein Pvs230 is a transmission-blocking vaccine candidate","volume":"30","author":"Tachibana","year":"2012","journal-title":"Vaccine"},{"key":"2024061323534067300_jiae102-B9","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.mib.2017.11.029","article-title":"Plasmodium P47: a key gene for malaria transmission by mosquito vectors","volume":"40","author":"Molina-Cruz","year":"2017","journal-title":"Curr Opin Microbiol"},{"key":"2024061323534067300_jiae102-B10","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.vaccine.2021.11.036","article-title":"Immunogenicity of full-length P. vivax rPvs48\/45 protein formulations in BALB\/c mice","volume":"40","author":"Arevalo-Herrera","year":"2022","journal-title":"Vaccine"},{"key":"2024061323534067300_jiae102-B11","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1016\/j.vaccine.2015.03.008","article-title":"Plasmodium vivax gametocyte proteins, Pvs48\/45 and Pvs47, induce transmission-reducing antibodies by DNA immunization","volume":"33","author":"Tachibana","year":"2015","journal-title":"Vaccine"},{"key":"2024061323534067300_jiae102-B12","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1016\/j.vaccine.2006.09.035","article-title":"The use of transgenic Plasmodium berghei expressing the Plasmodium vivax antigen P25 to determine the transmission-blocking activity of sera from malaria vaccine trials","volume":"25","author":"Ramjanee","year":"2007","journal-title":"Vaccine"},{"key":"2024061323534067300_jiae102-B13","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1128\/EC.00242-08","article-title":"Transgenic rodent Plasmodium berghei parasites as tools for assessment of functional immunogenicity and optimization of human malaria vaccines","volume":"7","author":"Mlambo","year":"2008","journal-title":"Eukaryot Cell"},{"key":"2024061323534067300_jiae102-B14","doi-asserted-by":"crossref","first-page":"e01627-18","DOI":"10.1128\/mBio.01627-18","article-title":"Functional conservation of P48\/45 proteins in the transmission stages of Plasmodium vivax (human malaria parasite) and P. berghei (murine malaria parasite)","volume":"9","author":"Cao","year":"2018","journal-title":"mBio"},{"key":"2024061323534067300_jiae102-B15","doi-asserted-by":"crossref","first-page":"6014","DOI":"10.1016\/j.vaccine.2010.06.100","article-title":"Intranasal and intramuscular immunization with baculovirus dual expression system-based Pvs25 vaccine substantially blocks Plasmodium vivax transmission","volume":"28","author":"Blagborough","year":"2010","journal-title":"Vaccine"},{"key":"2024061323534067300_jiae102-B16","doi-asserted-by":"crossref","first-page":"3252","DOI":"10.1016\/j.vaccine.2016.05.007","article-title":"Transmission blocking potency and immunogenicity of a plant-produced Pvs25-based subunit vaccine against Plasmodium vivax","volume":"34","author":"Blagborough","year":"2016","journal-title":"Vaccine"},{"key":"2024061323534067300_jiae102-B17","doi-asserted-by":"crossref","first-page":"4348","DOI":"10.1128\/IAI.02040-14","article-title":"Baculovirus-vectored multistage Plasmodium vivax vaccine induces both protective and transmission-blocking immunities against transgenic rodent malaria parasites","volume":"82","author":"Mizutani","year":"2014","journal-title":"Infect Immun"},{"key":"2024061323534067300_jiae102-B18","doi-asserted-by":"crossref","first-page":"2018","DOI":"10.1128\/IAI.01409-07","article-title":"Murine model for assessment of Plasmodium falciparum transmission-blocking vaccine using transgenic Plasmodium berghei parasites expressing the target antigen Pfs25","volume":"76","author":"Mlambo","year":"2008","journal-title":"Infect Immun"},{"key":"2024061323534067300_jiae102-B19","doi-asserted-by":"crossref","first-page":"7025","DOI":"10.1016\/j.vaccine.2010.08.022","article-title":"Functional immunogenicity of baculovirus expressing Pfs25, a human malaria transmission-blocking vaccine candidate antigen","volume":"28","author":"Mlambo","year":"2010","journal-title":"Vaccine"},{"key":"2024061323534067300_jiae102-B20","doi-asserted-by":"crossref","first-page":"3140","DOI":"10.1016\/j.vaccine.2013.05.006","article-title":"Functional evaluation of malaria Pfs25 DNA vaccine by in vivo electroporation in olive baboons","volume":"31","author":"Kumar","year":"2013","journal-title":"Vaccine"},{"key":"2024061323534067300_jiae102-B21","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1128\/CVI.00185-15","article-title":"Evaluation of the impact of codon optimization and N-linked glycosylation on functional immunogenicity of Pfs25 DNA vaccines delivered by in vivo electroporation in preclinical studies in mice","volume":"22","author":"Datta","year":"2015","journal-title":"Clin Vaccine Immunol"},{"key":"2024061323534067300_jiae102-B22","doi-asserted-by":"crossref","first-page":"e29428","DOI":"10.1371\/journal.pone.0029428","article-title":"A viral vectored prime-boost immunization regime targeting the malaria Pfs25 antigen induces transmission-blocking activity","volume":"6","author":"Goodman","year":"2011","journal-title":"PLoS One"},{"key":"2024061323534067300_jiae102-B23","doi-asserted-by":"crossref","first-page":"2412","DOI":"10.3389\/fimmu.2019.02412","article-title":"A viral-vectored multi-stage malaria vaccine regimen with protective and transmission-blocking efficacies","volume":"10","author":"Yusuf","year":"2019","journal-title":"Front Immunol"},{"key":"2024061323534067300_jiae102-B24","doi-asserted-by":"crossref","first-page":"730","DOI":"10.3389\/fimmu.2019.00730","article-title":"Adeno-associated virus as an effective malaria booster vaccine following adenovirus priming","volume":"10","author":"Yusuf","year":"2019","journal-title":"Front Immunol"},{"key":"2024061323534067300_jiae102-B25","doi-asserted-by":"crossref","first-page":"102652","DOI":"10.1016\/j.parint.2022.102652","article-title":"Adeno-associated virus-based malaria booster vaccine following attenuated replication-competent vaccinia virus LC16m8Delta priming","volume":"92","author":"Hasyim","year":"2023","journal-title":"Parasitol Int"},{"key":"2024061323534067300_jiae102-B26","doi-asserted-by":"crossref","first-page":"1005476","DOI":"10.3389\/fimmu.2022.1005476","article-title":"Sterile protection and transmission blockade by a multistage anti-malarial vaccine in the pre-clinical study","volume":"13","author":"Iyori","year":"2022","journal-title":"Front Immunol"},{"key":"2024061323534067300_jiae102-B27","doi-asserted-by":"crossref","first-page":"5187","DOI":"10.1016\/j.vaccine.2009.06.069","article-title":"Plasmodium berghei HAP2 induces strong malaria transmission-blocking immunity in vivo and in vitro","volume":"27","author":"Blagborough","year":"2009","journal-title":"Vaccine"},{"key":"2024061323534067300_jiae102-B28","doi-asserted-by":"crossref","first-page":"2868","DOI":"10.1016\/j.celrep.2017.11.024","article-title":"Targeting the conserved fusion loop of HAP2 inhibits the transmission of Plasmodium berghei and falciparum","volume":"21","author":"Angrisano","year":"2017","journal-title":"Cell Rep"},{"key":"2024061323534067300_jiae102-B29","doi-asserted-by":"crossref","first-page":"e74707","DOI":"10.7554\/eLife.74707","article-title":"Structural basis of malaria transmission blockade by a monoclonal antibody to gamete fusogen HAP2","volume":"10","author":"Feng","year":"2021","journal-title":"eLife"},{"key":"2024061323534067300_jiae102-B30","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.vaccine.2014.11.038","article-title":"The Plasmodium berghei sexual stage antigen PSOP12 induces anti-malarial transmission blocking immunity both in vivo and in vitro","volume":"33","author":"Sala","year":"2015","journal-title":"Vaccine"},{"key":"2024061323534067300_jiae102-B31","doi-asserted-by":"crossref","first-page":"7363","DOI":"10.1073\/pnas.1919709117","article-title":"PIMMS43 is required for malaria parasite immune evasion and sporogonic development in the mosquito vector","volume":"117","author":"Ukegbu","year":"2020","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2024061323534067300_jiae102-B32","doi-asserted-by":"crossref","first-page":"2134","DOI":"10.3389\/fmicb.2019.02134","article-title":"Fueling open innovation for malaria transmission-blocking drugs: hundreds of molecules targeting early parasite mosquito stages","volume":"10","author":"Delves","year":"2019","journal-title":"Front Microbiol"},{"key":"2024061323534067300_jiae102-B33","first-page":"10","article-title":"Heparin administered to anopheles in membrane feeding assays blocks Plasmodium development in the mosquito","volume":"11","author":"Lantero","year":"2020","journal-title":"Biomolecules"},{"key":"2024061323534067300_jiae102-B34","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1017\/S0031182000078628","article-title":"Sexual development in malarial parasites: gametocyte production, fertility and infectivity to the mosquito vector","volume":"100","author":"Dearsly","year":"1990","journal-title":"Parasitology"},{"key":"2024061323534067300_jiae102-B35","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1038\/nsmb1024","article-title":"The essential mosquito-stage P25 and P28 proteins from Plasmodium form tile-like triangular prisms","volume":"13","author":"Saxena","year":"2006","journal-title":"Nat Struct Mol Biol"},{"key":"2024061323534067300_jiae102-B36","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.3389\/fmicb.2020.01496","article-title":"In vivo characterization of Plasmodium berghei P47 (Pbs47) as a malaria transmission-blocking vaccine target","volume":"11","author":"Yenkoidiok-Douti","year":"2020","journal-title":"Front Microbiol"},{"key":"2024061323534067300_jiae102-B37","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1046\/j.1365-2567.1998.00486.x","article-title":"Evidence for epitope-specific thymus-independent response against a repeat sequence in a protein antigen","volume":"94","author":"Kumar","year":"1998","journal-title":"Immunology"},{"key":"2024061323534067300_jiae102-B38","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/s41541-022-00577-8","article-title":"mRNA-LNP expressing PfCSP and Pfs25 vaccine candidates targeting infection and transmission of Plasmodium falciparum","volume":"7","author":"Hayashi","year":"2022","journal-title":"NPJ Vaccines"},{"key":"2024061323534067300_jiae102-B39","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/1475-2875-6-107","article-title":"Transmission-blocking activity induced by malaria vaccine candidates Pfs25\/Pvs25 is a direct and predictable function of antibody titer","volume":"6","author":"Miura","year":"2007","journal-title":"Malar J"},{"key":"2024061323534067300_jiae102-B40","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1093\/trstmh\/trab095","article-title":"The effect of anticoagulants in blood collection tubes on Plasmodium falciparum transmission in direct membrane feeding assays","volume":"116","author":"Graumans","year":"2022","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"2024061323534067300_jiae102-B41","doi-asserted-by":"crossref","first-page":"242","DOI":"10.4269\/ajtmh.2007.77.242","article-title":"Effects of anticoagulants on Plasmodium vivax oocyst development in Anopheles albimanus mosquitoes","volume":"77","author":"Solarte","year":"2007","journal-title":"Am J Trop Med Hyg"},{"key":"2024061323534067300_jiae102-B42","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1016\/S0020-7519(01)00222-3","article-title":"Effect of xanthurenic acid on infectivity of Plasmodium falciparum to Anopheles stephensi","volume":"31","author":"Bhattacharyya","year":"2001","journal-title":"Int J Parasitol"},{"key":"2024061323534067300_jiae102-B43","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1186\/s12936-021-03725-y","article-title":"Maintaining Plasmodium falciparum gametocyte infectivity during blood collection and transport for mosquito feeding assays in the field","volume":"20","author":"Soumare","year":"2021","journal-title":"Malar J"},{"key":"2024061323534067300_jiae102-B44","doi-asserted-by":"crossref","first-page":"559","DOI":"10.2307\/3276326","article-title":"Effects of temperature on sporogonic development of Plasmodium berghei","volume":"52","author":"Vanderberg","year":"1966","journal-title":"J Parasitol"},{"key":"2024061323534067300_jiae102-B45","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1038\/32667","article-title":"Identification of xanthurenic acid as the putative inducer of malaria development in the mosquito","volume":"392","author":"Billker","year":"1998","journal-title":"Nature"},{"key":"2024061323534067300_jiae102-B46","doi-asserted-by":"crossref","first-page":"12003","DOI":"10.1074\/jbc.273.20.12003","article-title":"Xanthurenic acid induces gametogenesis in Plasmodium, the malaria parasite","volume":"273","author":"Garcia","year":"1998","journal-title":"J Biol Chem"},{"key":"2024061323534067300_jiae102-B47","doi-asserted-by":"crossref","first-page":"e0004807","DOI":"10.1371\/journal.pntd.0004807","article-title":"Optimization of a membrane feeding assay for Plasmodium vivax infection in Anopheles albimanus","volume":"10","author":"Vallejo","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"2024061323534067300_jiae102-B48","first-page":"10.3791\/61426","article-title":"Plasmodium falciparum gametocyte culture and mosquito infection through artificial membrane feeding","volume":"161","author":"Tripathi","year":"2020","journal-title":"J Vis Exp"},{"key":"2024061323534067300_jiae102-B49","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.actatropica.2017.08.027","article-title":"Modulation of transmission success of Plasmodium falciparum gametocytes (sexual stages) in various species of anopheles by erythrocytic asexual stage parasites","volume":"176","author":"Kumar","year":"2017","journal-title":"Acta Trop"}],"container-title":["The Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jid\/advance-article-pdf\/doi\/10.1093\/infdis\/jiae102\/57086379\/jiae102.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jid\/article-pdf\/229\/6\/1894\/58211810\/jiae102.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jid\/article-pdf\/229\/6\/1894\/58211810\/jiae102.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T13:40:46Z","timestamp":1718372446000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jid\/article\/229\/6\/1894\/7614182"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,26]]},"references-count":49,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,2,26]]},"published-print":{"date-parts":[[2024,6,14]]}},"URL":"https:\/\/doi.org\/10.1093\/infdis\/jiae102","relation":{},"ISSN":["0022-1899","1537-6613"],"issn-type":[{"type":"print","value":"0022-1899"},{"type":"electronic","value":"1537-6613"}],"subject":[],"published-other":{"date-parts":[[2024,6,15]]},"published":{"date-parts":[[2024,2,26]]}}}