{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T04:28:48Z","timestamp":1767155328325,"version":"3.41.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T00:00:00Z","timestamp":1711324800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","award":["2019-01439"],"award-info":[{"award-number":["2019-01439"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003793","name":"Hj\u00e4rt-Lungfonden","doi-asserted-by":"publisher","award":["20170258"],"award-info":[{"award-number":["20170258"]}],"id":[{"id":"10.13039\/501100003793","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Swedish Civil Contingency Agency"},{"DOI":"10.13039\/501100007294","name":"Myndigheten f\u00f6r Samh\u00e4llskydd och Beredskap","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007294","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007308","name":"Swedish Medical Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007308","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003252","name":"Lund University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003252","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Royal Physiographic Society"},{"DOI":"10.13039\/501100005753","name":"Kungliga Fysiografiska S\u00e4llskapet i Lund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005753","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Swedish Physicians Against AIDS Research Foundation"},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The role of ribonucleases in tuberculosis among people with human immunodeficiency virus (HIV; PWH) is unknown. We explored ribonuclease activity in plasma from PWH with and without tuberculosis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Participants were identified from a cohort of treatment-naive PWH in Ethiopia who had been classified for tuberculosis disease (HIV positive [HIV+]\/tuberculosis positive [tuberculosis+] or HIV+\/tuberculosis negative [tuberculosis\u2212]). Ribonuclease activity in plasma was investigated by quantification of synthetic spike-in RNAs using sequencing and quantitative polymerase chain reaction and by a specific ribonuclease activity assay. Quantification of ribonuclease 1, 2, 3, 6, 7, and T2 proteins was performed by enzyme-linked immunosorbent assay. Ribonuclease activity and protein concentrations were correlated with markers of tuberculosis and HIV disease severity and with concentrations of inflammatory mediators.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Ribonuclease activity was significantly higher in plasma of HIV+\/tuberculosis+ (n = 51) compared with HIV+\/tuberculosis\u2212 (n = 78), causing reduced stability of synthetic spike-in RNAs. Concentrations of ribonucleases 2, 3, and T2 were also significantly increased in HIV+\/tuberculosis+ compared with HIV+\/tuberculosis\u2212. Ribonuclease activity was correlated with HIV viral load, and inversely correlated with CD4 cell count, mid\u2013upper arm circumference, and body mass index. Moreover, ribonuclease activity was correlated with concentrations of interleukin 27, procalcitonin and the kynurenine-tryptophan ratio.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>PWH with tuberculosis disease have elevated plasma ribonuclease activity, which is also associated with HIV disease severity and systemic inflammation.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiae143","type":"journal-article","created":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T23:00:23Z","timestamp":1711062023000},"page":"403-410","source":"Crossref","is-referenced-by-count":1,"title":["Plasma Ribonuclease Activity in Antiretroviral Treatment\u2013Naive People With Human Immunodeficiency Virus and Tuberculosis Disease"],"prefix":"10.1093","volume":"230","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5106-9222","authenticated-orcid":false,"given":"Oskar","family":"Olsson","sequence":"first","affiliation":[{"name":"Clinical Infection Medicine, Department of Translational Medicine, Lund University , Malm\u00f6 ,","place":["Sweden"]},{"name":"Department of Infectious Diseases, Sk\u00e5ne University Hospital , Malm\u00f6\/Lund ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf","family":"S\u00f8kilde","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Translational Neuropsychiatry Unit , Aarhus University, Aarhus ,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fregenet","family":"Tesfaye","sequence":"additional","affiliation":[{"name":"Clinical Infection Medicine, Department of Translational Medicine, Lund University , Malm\u00f6 ,","place":["Sweden"]},{"name":"Armauer Hansen Research Institute , Addis Ababa ,","place":["Ethiopia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Karlson","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Lund University , Lund ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sten","family":"Skogmar","sequence":"additional","affiliation":[{"name":"Clinical Infection Medicine, Department of Translational Medicine, Lund University , Malm\u00f6 ,","place":["Sweden"]},{"name":"Department of Infectious Diseases, Sk\u00e5ne University Hospital , Malm\u00f6\/Lund ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6536-8146","authenticated-orcid":false,"given":"Marianne","family":"Jansson","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Lund University , Lund 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