{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T03:36:10Z","timestamp":1773804970872,"version":"3.50.1"},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T00:00:00Z","timestamp":1711324800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U01-AI114378"],"award-info":[{"award-number":["U01-AI114378"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U19-AI113170"],"award-info":[{"award-number":["U19-AI113170"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U01-AI162457"],"award-info":[{"award-number":["U01-AI162457"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["UL1-TR002553"],"award-info":[{"award-number":["UL1-TR002553"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["UL1-TR002489"],"award-info":[{"award-number":["UL1-TR002489"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI058607"],"award-info":[{"award-number":["AI058607"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>There is an urgent need for vaccines against Neisseria gonorrhoeae, the causative agent of gonorrhea. Vaccination with an outer membrane vesicle\u2013based Neisseria meningitidis vaccine provides some protection from N. gonorrhoeae; however, the mechanisms underlying this cross-protection are unknown. To address this need, we developed multiplexed bead-based assays for the relative quantification of human and mouse IgG and IgA against N gonorrhoeae antigens. The assays were evaluated for analyte independence, dilutional linearity, specificity, sensitivity, intra- and interassay variability, and robustness to sample storage conditions. The assay was then used to test samples from mice and humans immunized with an N meningitidis outer membrane vesicle vaccine.<\/jats:p>","DOI":"10.1093\/infdis\/jiae153","type":"journal-article","created":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T14:22:18Z","timestamp":1711376538000},"page":"852-856","source":"Crossref","is-referenced-by-count":4,"title":["Development and Validation of Multiplex Assays for Mouse and Human IgG and IgA to <i>Neisseria gonorrhoeae<\/i> Antigens"],"prefix":"10.1093","volume":"230","author":[{"given":"Erica L","family":"Stover","sequence":"first","affiliation":[{"name":"Duke Human Vaccine Institute, Department of Medicine, School of Medicine, Duke University , Durham, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marguerite B","family":"Little","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristie L","family":"Connolly","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Uniformed Services University , Bethesda, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lixin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University , Corvallis, Oregon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A","family":"Nicholas","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"},{"name":"Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra 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North Carolina at Chapel Hill, Chapel Hill, North Carolina"},{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5297-0207","authenticated-orcid":false,"given":"Andrew N","family":"Macintyre","sequence":"additional","affiliation":[{"name":"Duke Human Vaccine Institute, Department of Medicine, School of Medicine, Duke University , Durham, North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,3,25]]},"reference":[{"key":"2024101612563448200_jiae153-B1","doi-asserted-by":"crossref","first-page":"3187","DOI":"10.3389\/fimmu.2018.03187","article-title":"Human immune responses and the natural history of Neisseria gonorrhoeae infection","volume":"9","author":"Lovett","year":"2018","journal-title":"Front 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