{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:20:43Z","timestamp":1789996843010,"version":"4.0.1"},"reference-count":39,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T00:00:00Z","timestamp":1711411200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070130"],"award-info":[{"award-number":["32070130"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772148"],"award-info":[{"award-number":["81772148"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31820103002"],"award-info":[{"award-number":["31820103002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770144"],"award-info":[{"award-number":["31770144"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871624"],"award-info":[{"award-number":["81871624"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Distinguished Young Scholars of Tianjin","award":["20JCJQJC00180"],"award-info":[{"award-number":["20JCJQJC00180"]}]},{"name":"Natural Science Foundation (Key Project) of Tianjin","award":["20JCZDJC00820"],"award-info":[{"award-number":["20JCZDJC00820"]}]},{"name":"Natural Science Foundation of Shenzhen","award":["JCYJ20220530164604010"],"award-info":[{"award-number":["JCYJ20220530164604010"]}]},{"name":"Natural Science Foundation of Shenzhen","award":["JCYJ20230807151559009"],"award-info":[{"award-number":["JCYJ20230807151559009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Escherichia coli K1 is the leading cause of neonatal gram-negative bacterial meningitis, but the pathogenesis of E coli K1 meningitis remains unclear. Blood-brain barrier (BBB) penetration is a crucial step in E coli meningitis development. Here, we uncovered the crucial role of CsiR, a GntR family regulator, in E coli K1 virulence. During infection, csiR expression was induced due to the derepression by Fur in the blood and human brain microvascular endothelial cells (HBMECs). CsiR positively regulated ilvB expression, which is associated with branched chain amino acid synthesis. Furthermore, we revealed that IlvB activated the FAK\/PI3K pathway of HBMECs to induce actin cytoskeleton rearrangements, thereby promoting the bacterial invasion and penetration of the BBB. Overall, this study reveals a CsiR-mediated virulence regulation pathway in E coli K1, which may provide a useful target for the prevention or therapy of E coli meningitis.<\/jats:p>","DOI":"10.1093\/infdis\/jiae157","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T02:34:51Z","timestamp":1711506891000},"source":"Crossref","is-referenced-by-count":6,"title":["CsiR-Mediated Signal Transduction Pathway in Response to Low Iron Conditions Promotes <i>Escherichia coli<\/i> K1 Invasion and Penetration of the Blood-Brain Barrier"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3826-4586","authenticated-orcid":false,"given":"Yangyang","family":"Zheng","sequence":"first","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University , Tianjin , People's Republic of China"},{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education , Tianjin , People's Republic of China"},{"name":"Department of Microbiology, College of Life Sciences, Nankai University , Tianjin , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4427-0881","authenticated-orcid":false,"given":"Hao","family":"Sun","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University , Tianjin , People's Republic of China"},{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education , Tianjin , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanling","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University , Tianjin , People's Republic of China"},{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education , Tianjin , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Jin","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University , Tianjin , People's Republic of China"},{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education , Tianjin , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoya","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University , Tianjin , People's Republic of China"},{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education , Tianjin , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Pang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University , Tianjin , People's Republic of China"},{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education , Tianjin , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianwen","family":"Ge","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University , Tianjin , People's Republic of China"},{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education , Tianjin , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University , Tianjin , People's Republic of China"},{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education , Tianjin , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University , Tianjin , People's Republic of China"},{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education , Tianjin , People's Republic of China"},{"name":"Nankai International Advanced Research Institute , Shenzhen , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,3,26]]},"reference":[{"key":"2024041617045267200_jiae157-B1","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s00401-015-1531-z","article-title":"Host-pathogen interactions in bacterial meningitis","volume":"131","author":"Doran","year":"2016","journal-title":"Acta Neuropathol"},{"key":"2024041617045267200_jiae157-B2","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1038\/nrn1103","article-title":"Pathogenesis of bacterial meningitis: from bacteraemia to neuronal injury","volume":"4","author":"Kim","year":"2003","journal-title":"Nat Rev Neurosci"},{"key":"2024041617045267200_jiae157-B3","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1038\/nrmicro1952","article-title":"Mechanisms of microbial traversal of the 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