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Establishing continuous in vitro culture systems and suitable animal models is crucial for studying these pathogens. This review spotlights the Babesia duncani in culture-in mouse (ICIM) model as a promising resource for advancing research on the biology, pathogenicity, and virulence of intraerythrocytic parasites. The model offers practical benefits, encompassing well-defined culture conditions, ease of manipulation, and a well-annotated genome. Moreover, B. duncani serves as a surrogate system for drug discovery, facilitating the evaluation of new antiparasitic drugs in vitro and in animals, elucidating their modes of action, and uncovering potential resistance mechanisms. The B. duncani ICIM model thus emerges as a multifaceted tool with profound implications, promising advancements in our understanding of parasitic biology and shaping the development of future therapies.<\/jats:p>","DOI":"10.1093\/infdis\/jiae191","type":"journal-article","created":{"date-parts":[[2024,4,16]],"date-time":"2024-04-16T19:49:25Z","timestamp":1713296965000},"page":"263-270","source":"Crossref","is-referenced-by-count":3,"title":["<i>Babesia duncani<\/i>, a Model Organism for Investigating Intraerythrocytic Parasitism and Novel Antiparasitic Therapeutic Strategies"],"prefix":"10.1093","volume":"230","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1844-2101","authenticated-orcid":false,"given":"Tiffany","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Section of Infectious Diseases, Yale School of Medicine , New Haven, Connecticut , USA"},{"name":"Department of Microbial Pathogenesis, Yale School of Medicine , New Haven, Connecticut , USA"},{"name":"Department of Pathology, Yale School of Medicine , New Haven, Connecticut , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5028-1400","authenticated-orcid":false,"given":"Choukri","family":"Ben Mamoun","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Section of Infectious Diseases, Yale School of Medicine , New Haven, Connecticut , USA"},{"name":"Department of Microbial Pathogenesis, Yale School of Medicine , New Haven, Connecticut , USA"},{"name":"Department of Pathology, Yale School of Medicine , New Haven, Connecticut , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,4,16]]},"reference":[{"key":"2024072520133579900_jiae191-B1","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1182\/asheducation-2014.1.404","article-title":"RBCs as targets of 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