{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T17:09:48Z","timestamp":1793207388395,"version":"4.5.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"Supplement_2","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100008436","name":"National Foundation for Medical Research and Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008436","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006292","name":"Infectious Diseases Society of America","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006292","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Periodontitis is a chronic inflammatory disease driven by dysbiosis in subgingival microbial communities leading to increased abundance of a limited number of pathobionts, including Porphyromonas gingivalis and Treponema denticola. Oral health, particularly periodontitis, is a modifiable risk factor for Alzheimer disease (AD) pathogenesis, with components of both these bacteria identified in postmortem brains of persons with AD. Repeated oral inoculation of mice with P. gingivalis results in brain infiltration of bacterial products, increased inflammation, and induction of AD-like biomarkers. P. gingivalis displays synergistic virulence with T. denticola during periodontitis. The aim of the current study was to determine the ability of P. gingivalis and T. denticola, grown in physiologically relevant conditions, individually and in combination, to induce AD-like pathology following chronic oral inoculation of female mice over 12 weeks. P. gingivalis alone significantly increased all 7 brain pathologies examined: neuronal damage, activation of astrocytes and microglia, expression of inflammatory cytokines interleukin 1\u03b2 (IL-1\u03b2) and interleukin 6 and production of amyloid-\u03b2 plaques and hyperphosphorylated tau, in the hippocampus, cortex and midbrain, compared to control mice. T. denticola alone significantly increased neuronal damage, activation of astrocytes and microglia, and expression of IL-1\u03b2, in the hippocampus, cortex and midbrain, compared to control mice. Coinoculation of P. gingivalis with T. denticola significantly increased activation of astrocytes and microglia in the hippocampus, cortex and midbrain, and increased production of hyperphosphorylated tau and IL-1\u03b2 in the hippocampus only. The host brain response elicited by oral coinoculation was less than that elicited by each bacterium, suggesting coinoculation was less pathogenic.<\/jats:p>","DOI":"10.1093\/infdis\/jiae286","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T16:50:36Z","timestamp":1717001436000},"page":"S109-S116","source":"Crossref","is-referenced-by-count":23,"title":["Chronic Oral Inoculation of\n                    <i>Porphyromonas gingivalis<\/i>\n                    and\n                    <i>Treponema denticola<\/i>\n                    Induce Different Brain Pathologies in a Mouse Model of Alzheimer Disease"],"prefix":"10.1093","volume":"230","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2512-1047","authenticated-orcid":false,"given":"Giuseppe D","family":"Ciccotosto","sequence":"first","affiliation":[{"name":"Melbourne Dental School, The University of Melbourne , Melbourne , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0017-5233","authenticated-orcid":false,"given":"Ali I","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Melbourne Dental School, The University of Melbourne , Melbourne , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2170-4723","authenticated-orcid":false,"given":"Rita","family":"Paolini","sequence":"additional","affiliation":[{"name":"Melbourne Dental School, The University of Melbourne , Melbourne , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2478-9463","authenticated-orcid":false,"given":"Elly","family":"Bijlsma","sequence":"additional","affiliation":[{"name":"Melbourne Dental School, The University of Melbourne , Melbourne , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2952-806X","authenticated-orcid":false,"given":"Su","family":"Toulson","sequence":"additional","affiliation":[{"name":"Melbourne Dental School, The University of Melbourne , Melbourne , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6543-5281","authenticated-orcid":false,"given":"James","family":"Holden","sequence":"additional","affiliation":[{"name":"Melbourne Dental School, The University of Melbourne , Melbourne , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6618-4856","authenticated-orcid":false,"given":"Eric C","family":"Reynolds","sequence":"additional","affiliation":[{"name":"Melbourne Dental School, The University of Melbourne , Melbourne , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0106-8691","authenticated-orcid":false,"given":"Stuart G","family":"Dashper","sequence":"additional","affiliation":[{"name":"Melbourne Dental School, The University of Melbourne , Melbourne , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6371-3954","authenticated-orcid":false,"given":"Catherine A","family":"Butler","sequence":"additional","affiliation":[{"name":"Melbourne Dental School, The University of Melbourne , Melbourne , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,9,10]]},"reference":[{"key":"2024091017261071400_jiae286-B1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.5114\/fn.2019.85929","article-title":"Risk factors for Alzheimer's disease","volume":"57","author":"Armstrong","year":"2019","journal-title":"Folia 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