{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T14:35:36Z","timestamp":1768833336190,"version":"3.49.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T00:00:00Z","timestamp":1718323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1 AI141602"],"award-info":[{"award-number":["RO1 AI141602"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1 AI148802"],"award-info":[{"award-number":["RO1 AI148802"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U19AI129388"],"award-info":[{"award-number":["U19AI129388"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A hallmark of cerebral malaria is sequestration of Plasmodium falciparum-infected erythrocytes (IEs) in the brain microcirculation. Antibodies contribute to malaria immunity, but it remains unclear whether functional antibodies targeting parasite-expressed ligand can block cytoadhesion in the brain. Here, we screened the plasma of older children and young adults in Malawi to characterize the antibody response against the P. falciparum-IE surface and used a bioengineered 3-dimensional (3D) human brain microvessel model incorporating variable flow dynamics to measure adhesion-blocking responses. We found a strong correlation between surface antibody reactivity by flow cytometry and reduced P. falciparum-IE binding in 3D microvessels. Moreover, there was a threshold of surface antibody reactivity necessary to achieve robust inhibitory activity. Our findings provide evidence of the acquisition of adhesion-blocking antibodies against cerebral binding variants in people exposed to stable P. falciparum transmission and suggest the quality of the inhibitory response can be influenced by flow dynamics.<\/jats:p>","DOI":"10.1093\/infdis\/jiae315","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T17:11:36Z","timestamp":1718385096000},"page":"e1402-e1411","source":"Crossref","is-referenced-by-count":8,"title":["Plasma From Older Children in Malawi Inhibits <i>Plasmodium falciparum<\/i> Binding in 3-Dimensional Brain Microvessels"],"prefix":"10.1093","volume":"230","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9731-9239","authenticated-orcid":false,"given":"Fatou","family":"Joof","sequence":"first","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children's Research Institute , Seattle, Washington ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruoqian","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of Washington , Seattle, Washington ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Saidi","sequence":"additional","affiliation":[{"name":"Blantyre Malaria Project, Kamuzu University of Health Sciences , Blantyre ,","place":["Malawi"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl B","family":"Seydel","sequence":"additional","affiliation":[{"name":"Blantyre Malaria Project, Kamuzu University of Health Sciences , Blantyre ,","place":["Malawi"]},{"name":"Department of Osteopathic Medical Specialties, College of Osteopathic Medicine, Michigan State University , East Lansing, Michigan ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren M","family":"Cohee","sequence":"additional","affiliation":[{"name":"Center for Vaccine Development and Global Health, University of Maryland School of Medicine , Baltimore, Maryland ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2723-9022","authenticated-orcid":false,"given":"Ying","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of Washington , Seattle, Washington ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7915-6360","authenticated-orcid":false,"given":"Joseph D","family":"Smith","sequence":"additional","affiliation":[{"name":"Center for Global Infectious Disease Research, Seattle Children's Research Institute , Seattle, Washington ,","place":["USA"]},{"name":"Department of Pediatrics, School of Medicine, University of Washington , Seattle, Washington 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