{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T01:59:33Z","timestamp":1783735173494,"version":"3.55.0"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T00:00:00Z","timestamp":1724976000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Clinical Virology Unit"},{"DOI":"10.13039\/100016015","name":"University Hospital Basel","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016015","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In recipients of allogeneic hematopoietic cell transplant, prophylactic management strategies are essential for preventing cytomegalovirus (CMV) reactivation and associated disease. We report on a 63-year-old male patient with a D\u2212\/R+ CMV serostatus, who showed ongoing low-level CMV replication after hematopoietic cell transplant despite receiving letermovir prophylaxis. Sanger sequencing failed to detect drug resistance mutations (DRMs) until CMV pneumonitis developed, revealing a UL56-C325R DRM linked to high-level letermovir resistance. Retrospective analysis with next-generation sequencing revealed the DRM at a low frequency of 6% two weeks prior to detection by Sanger sequencing. This study highlights the importance of advanced next-generation sequencing methods for early detection of CMV DRMs, allowing for faster adjustments in antiviral treatment strategies.<\/jats:p>","DOI":"10.1093\/infdis\/jiae414","type":"journal-article","created":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T05:35:05Z","timestamp":1724996105000},"page":"e177-e182","source":"Crossref","is-referenced-by-count":8,"title":["Revealing the Unseen: Next-Generation Sequencing for Early Detection of Drug-Resistant Cytomegalovirus Variants Upon Letermovir Prophylaxis Failure"],"prefix":"10.1093","volume":"231","author":[{"given":"Klaudia","family":"N\u00e4gele","sequence":"first","affiliation":[{"name":"Clinical Virology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"B\u00e4ttig","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Hospital 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