{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T06:43:01Z","timestamp":1793256181912,"version":"4.5.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T00:00:00Z","timestamp":1726704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Seroprevalence studies are the standard for disease surveillance, and serology determined eligibility for the first dengue vaccine. Expanding flavivirus co-circulation and vaccination complicate testing. We evaluate the accuracy of a common dengue virus serological assay, examine immunity to non-dengue flaviviruses as a contributor to decreased performance, and assess whether alternative cut points may improve assay performance.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Children (n = 770) aged 2\u20139 years in Kampong Speu, Cambodia were enrolled in a prospective longitudinal study, and PanBio indirect dengue virus immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) was performed. Plaque reduction neutralization tests (PRNTs) using dengue viruses were performed on a subset to assess the accuracy of the IgG ELISA, and PRNTs with Zika, Japanese encephalitis, and West Nile viruses evaluated immunity to non-dengue flaviviruses. Receiver operating curve analysis identified an alternative cut point to improve IgG ELISA accuracy.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The dengue IgG ELISA had a lower specificity than previously reported (58% vs 93%\u2013100%). Of those with false-positive IgG results, 46% had detectable neutralizing antibodies against other flaviviruses including 14% against West Nile virus. A higher IgG cut point improved the test accuracy in this population.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Physicians and public health authorities should be alert for West Nile in Cambodia. Immunity to non-dengue flaviviruses can impact dengue surveillance.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Clinical Trials Registration<\/jats:title>\n                  <jats:p>NCT03534245.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiae422","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T13:20:15Z","timestamp":1726752015000},"page":"e337-e344","source":"Crossref","is-referenced-by-count":1,"title":["Immunity to Non-Dengue Flaviviruses Impacts Dengue Virus Immunoglobulin G Enzyme-Linked Immunosorbent Assay Specificity in Cambodia"],"prefix":"10.1093","volume":"231","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1730-4380","authenticated-orcid":false,"given":"Camila D","family":"Odio","sequence":"first","affiliation":[{"name":"Viral Epidemiology and Immunity Unit, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8293-3901","authenticated-orcid":false,"given":"Christina","family":"Yek","sequence":"additional","affiliation":[{"name":"International Center of Excellence in Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Phnom Penh ,","place":["Cambodia"]},{"name":"Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chloe M","family":"Hasund","sequence":"additional","affiliation":[{"name":"Viral Epidemiology and Immunity Unit, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somnang","family":"Man","sequence":"additional","affiliation":[{"name":"International Center of Excellence in Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Phnom Penh ,","place":["Cambodia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piseth","family":"Ly","sequence":"additional","affiliation":[{"name":"International Center of Excellence in Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Phnom Penh ,","place":["Cambodia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sreynik","family":"Nhek","sequence":"additional","affiliation":[{"name":"International Center of Excellence in Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Phnom Penh ,","place":["Cambodia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3187-3691","authenticated-orcid":false,"given":"Sophana","family":"Chea","sequence":"additional","affiliation":[{"name":"International Center of Excellence in Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Phnom Penh ,","place":["Cambodia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanthap","family":"Lon","sequence":"additional","affiliation":[{"name":"International Center of Excellence in Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Phnom Penh ,","place":["Cambodia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlie","family":"Voirin","sequence":"additional","affiliation":[{"name":"Viral Epidemiology and Immunity Unit, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National 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