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We compared the frequency of memory T-cell populations in breast milk between mothers of infants who acquired breast milk HCMV (transmitters) and those with uninfected infants (nontransmitters). Nontransmitter mothers had an increased frequency of CD8+ effector memory T-cells (Tem) in their breast milk. In addition, we describe tissue-resident memory T cells (Trm) in breast milk and demonstrate most were Tem. We present data that nontransmitter mothers have increased frequency of CD8+ Trm in their breast milk when compared to transmitters and that Trm frequency is inversely correlated with breast milk HCMV viral load. Lastly, using scRNA-seq analysis we identified a unique population of T-cells in nontransmitters and demonstrate that these cells comprise CD8+ Trm. These studies suggest that CD8+ Trm may play a role in preventing viral transmission via breast milk to infants.<\/jats:p>","DOI":"10.1093\/infdis\/jiae618","type":"journal-article","created":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T22:25:08Z","timestamp":1733955908000},"page":"1327-1335","source":"Crossref","is-referenced-by-count":3,"title":["Tissue-Resident CD8+ T Cells as Mediators of Protective Immunity in Breast Milk Transmission of Human Cytomegalovirus"],"prefix":"10.1093","volume":"231","author":[{"given":"Shara B","family":"Legg","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Alabama at Birmingham , Birmingham, Alabama ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David C","family":"Moylan","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alabama at Birmingham , Birmingham, Alabama ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neema","family":"Kamau","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alabama at Birmingham , Birmingham, Alabama ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8625-1948","authenticated-orcid":false,"given":"Misty P","family":"Latting","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Alabama at Birmingham , Birmingham, Alabama ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1281-9330","authenticated-orcid":false,"given":"Dhruv","family":"Devdhara","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alabama at Birmingham , Birmingham, Alabama ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunil K","family":"Pati","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Alabama at Birmingham , Birmingham, Alabama ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0981-169X","authenticated-orcid":false,"given":"David K","family":"Crossman","sequence":"additional","affiliation":[{"name":"Department of Genetics, University of Alabama at Birmingham , Birmingham, Alabama ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4785-2767","authenticated-orcid":false,"given":"Suresh B","family":"Boppana","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Alabama at Birmingham , Birmingham, Alabama ,","place":["USA"]},{"name":"Department of Microbiology, University of Alabama at Birmingham , Birmingham, Alabama 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