{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T20:58:30Z","timestamp":1769633910829,"version":"3.49.0"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T00:00:00Z","timestamp":1744934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008385","name":"Italian Cystic Fibrosis Research Foundation","doi-asserted-by":"publisher","award":["FFC 13\/2022"],"award-info":[{"award-number":["FFC 13\/2022"]}],"id":[{"id":"10.13039\/501100008385","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Rome Technopole","award":["ESC00000024"],"award-info":[{"award-number":["ESC00000024"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The study investigates therapeutic strategies for managing chronic Mycobacterium abscessus infections, particularly in people with cystic fibrosis (PWCF) who are ineligible for standard elexacaftor, tezacaftor, ivakaftor (ETI) treatments. Apoptotic body-like liposomes loaded with phosphatidylinositol 5-phosphate (ABL\/PI5P) were tested in vitro in M. abscessus-infected macrophages from PWCF as potential treatment. ABL\/PI5P reduced intracellular bacterial viability and showed enhanced effects on a M. abscessus clinical strain when combined with amikacin. Notably, ABL\/PI5P was effective on macrophages from PWCF not receiving ETI therapy. The findings suggest ABL\/PI5P liposomes as a promising alternative or adjunct therapy, especially for those who cannot access ETI treatment, warranting further clinical investigation.<\/jats:p>","DOI":"10.1093\/infdis\/jiaf124","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T07:36:17Z","timestamp":1744356977000},"page":"e43-e47","source":"Crossref","is-referenced-by-count":1,"title":["Phosphatidylinositol 5-Phosphate-Loaded Apoptotic Body-Like Liposomes for <i>Mycobacterium abscessus<\/i> Infection Management in Patients With Cystic Fibrosis"],"prefix":"10.1093","volume":"232","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3058-2844","authenticated-orcid":false,"given":"Tommaso","family":"Olimpieri","sequence":"first","affiliation":[{"name":"Department of Biology, University of Rome Tor Vergata , Rome ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noemi","family":"Poerio","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome Tor Vergata , Rome ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8446-4763","authenticated-orcid":false,"given":"Fabio","family":"Saliu","sequence":"additional","affiliation":[{"name":"Emerging Bacteria Pathogens Unit, San Raffaele Scientific Institute , Milan ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola I","family":"Lor\u00e8","sequence":"additional","affiliation":[{"name":"Emerging Bacteria Pathogens Unit, San Raffaele Scientific Institute , Milan ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1493-9767","authenticated-orcid":false,"given":"Fabiana","family":"Ciciriello","sequence":"additional","affiliation":[{"name":"Pneumology and Cystic Fibrosis Unit, Bambino Ges\u00f9 Children's Hospital, Scientific Institute for Research Hospitalization and Healthcare (IRCCS) , Rome ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greta","family":"Ponsecchi","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome Tor Vergata , Rome ,","place":["Italy"]},{"name":"PhD Program in Evolutionary Biology and Ecology, Department of Biology, University of Rome Tor Vergata , Rome ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco M","family":"D\u2019Andrea","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome Tor Vergata , Rome ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4333-791X","authenticated-orcid":false,"given":"Federico","family":"Alghisi","sequence":"additional","affiliation":[{"name":"Pneumology and Cystic Fibrosis Unit, Bambino Ges\u00f9 Children's Hospital, Scientific Institute for Research Hospitalization and Healthcare (IRCCS) , Rome ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela M","family":"Cirillo","sequence":"additional","affiliation":[{"name":"Emerging Bacteria Pathogens Unit, San Raffaele Scientific Institute , Milan ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5643-0769","authenticated-orcid":false,"given":"Maurizio","family":"Fraziano","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome Tor Vergata , Rome ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,4,18]]},"reference":[{"key":"2025073007425497300_jiaf124-B1","doi-asserted-by":"crossref","first-page":"17066","DOI":"10.1038\/s41598-018-35151-7","article-title":"Cystic fibrosis transmembrane conductance regulator (CFTR) modulators have differential effects on cystic fibrosis macrophage function","volume":"8","author":"Zhang","year":"2018","journal-title":"Sci Rep"},{"issue":"Suppl 1","key":"2025073007425497300_jiaf124-B2","doi-asserted-by":"crossref","first-page":"i1","DOI":"10.1136\/thoraxjnl-2015-207360","article-title":"US Cystic Fibrosis Foundation and European Cystic Fibrosis Society consensus recommendations for the management of non-tuberculous mycobacteria in individuals with cystic fibrosis","volume":"71","author":"Floto","year":"2016","journal-title":"Thorax"},{"key":"2025073007425497300_jiaf124-B3","doi-asserted-by":"crossref","first-page":"45120","DOI":"10.1038\/srep45120","article-title":"Liposomes loaded with bioactive lipids enhance antibacterial innate immunity irrespective of drug resistance","volume":"7","author":"Poerio","year":"2017","journal-title":"Sci Rep"},{"key":"2025073007425497300_jiaf124-B4","doi-asserted-by":"crossref","first-page":"e0254621","DOI":"10.1128\/spectrum.02546-21","article-title":"Combined host- and pathogen-directed therapy for the control of Mycobacterium abscessus infection","volume":"10","author":"Poerio","year":"2022","journal-title":"Microbiol Spectr"},{"key":"2025073007425497300_jiaf124-B5","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1038\/s41467-023-36244-2","article-title":"Future therapies for cystic fibrosis","volume":"14","author":"Allen","year":"2023","journal-title":"Nat Commun"},{"key":"2025073007425497300_jiaf124-B6","doi-asserted-by":"crossref","first-page":"606","DOI":"10.3390\/ph15050606","article-title":"Elexacaftor-tezacaftor-ivacaftor as a final frontier in the treatment of cystic fibrosis: definition of the clinical and microbiological implications in a case-control study","volume":"15","author":"Migliorisi","year":"2022","journal-title":"Pharmaceuticals (Basel)"},{"key":"2025073007425497300_jiaf124-B7","doi-asserted-by":"crossref","first-page":"1602525","DOI":"10.1183\/13993003.02525-2016","article-title":"Mycobacterium abscessus in patients with cystic fibrosis: low impact of inter-human transmission in Italy","volume":"50","author":"Tortoli","year":"2017","journal-title":"Eur Respir J"},{"key":"2025073007425497300_jiaf124-B8","author":"Vertex Pharmaceuticals"},{"key":"2025073007425497300_jiaf124-B9","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1007\/s40262-021-01010-4","article-title":"Clinical pharmacokinetic and pharmacodynamic considerations in the drug treatment of non-tuberculous mycobacteria in cystic fibrosis","volume":"60","author":"Burke","year":"2021","journal-title":"Clin Pharmacokinet"},{"key":"2025073007425497300_jiaf124-B10","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1093\/cid\/ciac117","article-title":"The rapid reduction of infection-related visits and antibiotic use among people with cystic fibrosis after starting elexacaftor-tezacaftor-ivacaftor","volume":"75","author":"Miller","year":"2022","journal-title":"Clin Infect Dis"},{"key":"2025073007425497300_jiaf124-B11","author":"European Medicines Agency"},{"key":"2025073007425497300_jiaf124-B12","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1378\/chest.11-2348","article-title":"Defective phagocytosis in airways disease","volume":"141","author":"Donnelly","year":"2012","journal-title":"Chest"},{"key":"2025073007425497300_jiaf124-B13","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1038\/ncb1456","article-title":"CFTR regulates phagosome acidification in macrophages and alters bactericidal activity","volume":"8","author":"Di","year":"2006","journal-title":"Nat Cell Biol"},{"key":"2025073007425497300_jiaf124-B14","author":"Zolin","year":"2024"},{"key":"2025073007425497300_jiaf124-B15","author":"Cystic Fibrosis Foundation"}],"container-title":["The Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jid\/advance-article-pdf\/doi\/10.1093\/infdis\/jiaf124\/62953373\/jiaf124.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jid\/article-pdf\/232\/1\/e43\/62953373\/jiaf124.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jid\/article-pdf\/232\/1\/e43\/62953373\/jiaf124.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:43:02Z","timestamp":1753875782000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jid\/article\/232\/1\/e43\/8116030"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,18]]},"references-count":15,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,4,18]]},"published-print":{"date-parts":[[2025,7,30]]}},"URL":"https:\/\/doi.org\/10.1093\/infdis\/jiaf124","relation":{},"ISSN":["0022-1899","1537-6613"],"issn-type":[{"value":"0022-1899","type":"print"},{"value":"1537-6613","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,7,15]]},"published":{"date-parts":[[2025,4,18]]}}}