{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T06:34:28Z","timestamp":1792823668998,"version":"4.5.0"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T00:00:00Z","timestamp":1744848000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U19AI089680"],"award-info":[{"award-number":["U19AI089680"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K24AI134990"],"award-info":[{"award-number":["K24AI134990"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI139520"],"award-info":[{"award-number":["R01AI139520"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32AI138953-03"],"award-info":[{"award-number":["T32AI138953-03"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015283","name":"Bloomberg Philanthropies","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015283","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011780","name":"Johns Hopkins Malaria Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Increasing artemisinin partial resistance (ART-R) due to mutations in the gene encoding Kelch13 (Pfk13) protein in eastern Africa is of urgent concern, and mutations, such as Pfk13 P441L, continue to emerge. We used an amplicon deep-sequencing panel to estimate the prevalence of validated and candidate ART-R Pfk13 mutations in samples collected between 2018 and 2023 in southern Zambia. Pfk13 P441L was present in 30 of 501 samples (6%), and prevalence increased over time (0% to 7.2%). Further studies of the P441L mutation are needed to document its geographical origin, distribution, and impact on treatment outcomes.<\/jats:p>","DOI":"10.1093\/infdis\/jiaf188","type":"journal-article","created":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T02:49:38Z","timestamp":1744858178000},"page":"918-922","source":"Crossref","is-referenced-by-count":10,"title":["Emergence and Rising Prevalence of Artemisinin Partial Resistance Marker Kelch13 P441L in a Low Malaria Transmission Setting in Southern Zambia"],"prefix":"10.1093","volume":"232","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0068-6519","authenticated-orcid":false,"given":"Anne C","family":"Martin","sequence":"first","affiliation":[{"name":"Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob M","family":"Sadler","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of North Carolina , Chapel Hill, North Carolina ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9408-0053","authenticated-orcid":false,"given":"Alfred","family":"Simkin","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Brown University , Providence, Rhode Island ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Musonda","sequence":"additional","affiliation":[{"name":"Macha Research Trust , Choma ,","place":["Zambia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8935-9196","authenticated-orcid":false,"given":"Ben","family":"Katowa","sequence":"additional","affiliation":[{"name":"Macha Research Trust , Choma ,","place":["Zambia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6021-659X","authenticated-orcid":false,"given":"Japhet","family":"Matoba","sequence":"additional","affiliation":[{"name":"Macha Research Trust , Choma ,","place":["Zambia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5188-5692","authenticated-orcid":false,"given":"Jessica","family":"Schue","sequence":"additional","affiliation":[{"name":"Department of International Health, Johns Hopkins Bloomberg School of Public Health , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9423-0816","authenticated-orcid":false,"given":"Edgar","family":"Simulundu","sequence":"additional","affiliation":[{"name":"Macha Research Trust , Choma ,","place":["Zambia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey A","family":"Bailey","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Brown University , Providence, Rhode Island ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J","family":"Moss","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health , Baltimore, Maryland ,","place":["USA"]},{"name":"Department of International Health, Johns Hopkins Bloomberg School of Public Health , Baltimore, Maryland ,","place":["USA"]},{"name":"Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health , Baltimore, Maryland ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0591-0850","authenticated-orcid":false,"given":"Jonathan J","family":"Juliano","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, School of Medicine, University of North Carolina , Chapel Hill, North Carolina ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abebe A","family":"Fola","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Brown University , Providence, Rhode Island ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,4,17]]},"reference":[{"key":"2025101515140220100_jiaf188-B1","author":"World Health Organization"},{"key":"2025101515140220100_jiaf188-B2","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/S1473-3099(17)30048-8","article-title":"The spread of artemisinin-resistant Plasmodium falciparum in the greater Mekong subregion: a molecular epidemiology observational study","volume":"17","author":"Imwong","year":"2017","journal-title":"Lancet Infect Dis"},{"key":"2025101515140220100_jiaf188-B3","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.ijpddr.2021.06.001","article-title":"A review of the frequencies of Plasmodium falciparum kelch 13 artemisinin resistance mutations in Africa","volume":"16","author":"Ndwiga","year":"2021","journal-title":"Int J Parasitol Drugs Drug Resist"},{"key":"2025101515140220100_jiaf188-B4","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1056\/NEJMoa2211803","article-title":"Evolution of partial resistance to artemisinins in malaria parasites in Uganda","volume":"389","author":"Conrad","year":"2023","journal-title":"N Engl J Med"},{"key":"2025101515140220100_jiaf188-B5","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1093\/infdis\/jiae475","article-title":"High frequency of artemisinin partial resistance mutations in the great lakes region revealed through rapid pooled deep sequencing","volume":"231","author":"Wernsman Young","year":"2025","journal-title":"J Infect Dis"},{"key":"2025101515140220100_jiaf188-B6","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s12936-023-04812-y","article-title":"Molecular surveillance of Kelch 13 polymorphisms in Plasmodium falciparum isolates from Kenya and Ethiopia","volume":"23","author":"Jeang","year":"2024","journal-title":"Malar J"},{"key":"2025101515140220100_jiaf188-B7","doi-asserted-by":"publisher","author":"Brhane","DOI":"10.1101\/2024.09.11.24313421"},{"key":"2025101515140220100_jiaf188-B8","doi-asserted-by":"publisher","author":"Fola","DOI":"10.1101\/2024.08.05.24311512"},{"key":"2025101515140220100_jiaf188-B9","doi-asserted-by":"crossref","first-page":"e77634","DOI":"10.7554\/eLife.77634","article-title":"The influence of biological, epidemiological, and treatment factors on the establishment and spread of drug-resistant Plasmodium falciparum.","volume":"11","author":"Masserey","year":"2022","journal-title":"eLife"},{"key":"2025101515140220100_jiaf188-B10","doi-asserted-by":"publisher","author":"Sadler","DOI":"10.1101\/2024.10.03.24314715"},{"key":"2025101515140220100_jiaf188-B11","doi-asserted-by":"crossref","first-page":"2453","DOI":"10.1056\/NEJMoa1513137","article-title":"A worldwide map of Plasmodium falciparum K13-propeller polymorphisms","volume":"374","author":"M\u00e9nard","year":"2016","journal-title":"N Engl J Med"},{"key":"2025101515140220100_jiaf188-B12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12916-018-1207-3","article-title":"Association of mutations in the Plasmodium falciparum Kelch13 gene (Pf3D7_1343700) with parasite clearance rates after artemisinin-based treatments\u2014a WWARN individual patient data meta-analysis","volume":"17","author":"WWARN K13 Genotype-Phenotype Study Group","year":"2019","journal-title":"BMC Med"},{"key":"2025101515140220100_jiaf188-B13","doi-asserted-by":"publisher","author":"Connelly","DOI":"10.1101\/2025.03.22.25323829"},{"key":"2025101515140220100_jiaf188-B14","doi-asserted-by":"publisher","author":"Eloff","DOI":"10.1101\/2025.01.09.25320247"},{"key":"2025101515140220100_jiaf188-B15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12936-024-04955-6","article-title":"Molecular markers of artemisinin resistance during falciparum malaria elimination in eastern Myanmar","volume":"23","author":"Thu","year":"2024","journal-title":"Malar J"}],"container-title":["The Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jid\/advance-article-pdf\/doi\/10.1093\/infdis\/jiaf188\/62942349\/jiaf188.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jid\/article-pdf\/232\/4\/918\/62942349\/jiaf188.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jid\/article-pdf\/232\/4\/918\/62942349\/jiaf188.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T19:15:50Z","timestamp":1760555750000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jid\/article\/232\/4\/918\/8114928"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,17]]},"references-count":15,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4,17]]},"published-print":{"date-parts":[[2025,10,15]]}},"URL":"https:\/\/doi.org\/10.1093\/infdis\/jiaf188","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.01.02.24319706","asserted-by":"object"}]},"ISSN":["0022-1899","1537-6613"],"issn-type":[{"value":"0022-1899","type":"print"},{"value":"1537-6613","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,10,15]]},"published":{"date-parts":[[2025,4,17]]}}}