{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:45:18Z","timestamp":1791852318845,"version":"4.3.4"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Molecular epidemiological surveillance for zoonotic strongyloidiasis is confounded by a genus-specific TaqMan probe assay that conflates Strongyloides fuelleborni fuelleborni with Strongyloides stercoralis. To improve surveillance, we developed and validated a novel duplex species-specific TaqMan probe assay, screening a representative collection of available clinical samples. Our assay was highly discriminatory, evidencing no cross-reactivity, and had a lowest DNA detection limit of 1\u2005pg\/\u00b5L. However, as the genus-specific DNA assay has greater detection ability, we propose a 2-step protocol where samples are first screened with this assay then, if positive, screened with our species-specific assay, discriminating (co)infections between each threadworm species.<\/jats:p>","DOI":"10.1093\/infdis\/jiaf237","type":"journal-article","created":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T07:29:44Z","timestamp":1746689384000},"page":"e169-e173","source":"Crossref","is-referenced-by-count":9,"title":["Improving Molecular Epidemiological Surveillance of Strongyloidiasis Upon Differentiation of\n                    <i>Strongyloides fuelleborni fuelleborni<\/i>\n                    From\n                    <i>Strongyloides stercoralis<\/i>"],"prefix":"10.1093","volume":"232","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3017-4074","authenticated-orcid":false,"given":"Lucas J","family":"Cunningham","sequence":"first","affiliation":[{"name":"Department of Tropical Disease 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Elisabeth-Tweesteden Hospital , Tilburg ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0108-6822","authenticated-orcid":false,"given":"Dora","family":"Buonfrate","sequence":"additional","affiliation":[{"name":"Department of Infectious, Tropical Diseases and Microbiology, Istituto di Ricovero e Cura a Carattere Scientifico Sacro Cuore Don Calabria Hospital , Verona ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2956-8997","authenticated-orcid":false,"given":"Salvatore","family":"Scarso","sequence":"additional","affiliation":[{"name":"Department of Infectious, Tropical Diseases and Microbiology, Istituto di Ricovero e Cura a Carattere Scientifico Sacro Cuore Don Calabria Hospital , Verona 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