{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T22:24:46Z","timestamp":1782253486588,"version":"3.54.5"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Patient-Centered Clinical Research Coordinating Center","award":["RS-2021-KH119793"],"award-info":[{"award-number":["RS-2021-KH119793"]}]},{"name":"Korea Health Technology R&D Project"},{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["RS-2024-00456525"],"award-info":[{"award-number":["RS-2024-00456525"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Secreted chorismate mutase (S-CM) is known to act as a virulence factor in mycobacterial infections by inhibiting macrophage apoptosis. This study aims to investigate genetic variations in S-CM among Mycobacterium intracellulare strains and evaluate their influence on the clinical progression of M intracellulare pulmonary disease (PD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Patients diagnosed with M intracellulare PD who received treatment between 1 January 2020 and 31 December 2023 at Seoul National University Hospital were included. Clinical isolates collected at treatment initiation were assessed for subspecies classification and S-CM genetic variations. Treatment outcomes were analyzed based on subspecies and S-CM mutation status. J774A.1 murine macrophages were infected with type strains (ATCC13950T) carrying S-CM expression plasmids and clinical isolates. Macrophage apoptosis and bacterial colony-forming units (CFUs) were quantified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Among the 118 isolates, 57 were identified as M intracellulare subspecies intracellulare (typical M intracellulare [TMI]), 53 as Mycobacterium paraintracellulare, and 8 as other subspecies. A C276A mutation causing S-CM truncation was detected in 24 isolates (20.3%), all belonging to TMI. TMI strains with S-CM truncation demonstrated a higher rate of culture conversion than those with intact S-CM (adjusted hazard ratio, 2.17 [95% confidence interval, 1.08\u20134.34]; P = .029). In murine macrophages, TMI strains with truncated S-CM induced higher levels of apoptosis and lower CFUs, whereas expressing intact S-CM in a type strain with naturally truncated S-CM reduced apoptosis and increased bacterial burden.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>S-CM truncation in M intracellulare enhances macrophage apoptosis, yielding reduced bacterial burden, decreased pathogenicity, and improved treatment responses.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/infdis\/jiaf360","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T07:55:19Z","timestamp":1751529319000},"page":"1365-1374","source":"Crossref","is-referenced-by-count":2,"title":["Secreted Chorismate Mutase: A Novel Prognostic Factor in\n                    <i>Mycobacterium intracellulare<\/i>\n                    Pulmonary Disease"],"prefix":"10.1093","volume":"232","author":[{"given":"Juye","family":"Bae","sequence":"first","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University College of Medicine , Seoul ,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju-Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Seoul National University College of Medicine , Seoul ,","place":["South Korea"]},{"name":"Department of Biomedical Sciences, College of Medicine, Seoul National University , Seoul ,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyejun","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Seoul National University College of Medicine , Seoul ,","place":["South Korea"]},{"name":"Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC) , Seoul ,","place":["South Korea"]},{"name":"Cancer Research Institute, College of Medicine, Seoul National University , Seoul ,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4444-508X","authenticated-orcid":false,"given":"Jake","family":"Whang","sequence":"additional","affiliation":[{"name":"Department of Research and Development, Korean Institute of Tuberculosis , Osong ,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8272-3178","authenticated-orcid":false,"given":"Joong-Yub","family":"Kim","sequence":"additional","affiliation":[{"name":"Biomedical Research Institute, Seoul National University Hospital , Seoul ,","place":["South Korea"]},{"name":"Department of Microbiology, Harvard Medical School , Boston, Massachusetts 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Immunology, Seoul National University , Hongcheon ,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1897-946X","authenticated-orcid":false,"given":"Nakwon","family":"Kwak","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University College of Medicine , Seoul ,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"key":"2026031304465246400_jiaf360-B1","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1093\/cid\/ciaa241","article-title":"Treatment of nontuberculous mycobacterial pulmonary disease: an official ATS\/ERS\/ESCMID\/IDSA clinical practice guideline","volume":"71","author":"Daley","year":"2020","journal-title":"Clin Infect 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