{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T00:06:26Z","timestamp":1793232386204,"version":"4.5.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T00:00:00Z","timestamp":1756166400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["178189"],"award-info":[{"award-number":["178189"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Listeria monocytogenes spreads intercellularly by creating actin-rich projections that are endocytosed into recipient cells. Caveolin-mediated endocytosis has been implicated in this process, accounting for about 70% in cell-to-cell spread in cells depleted of caveolin-1. Thus, additional mechanisms may contribute to the remaining spread, and we examined the role of flotillin-based endocytosis. We found that flotillin-1 localized to L. monocytogenes invaginations in recipient cells and depletion of flotillin-1 significantly impaired bacterial transfer. Similarly, preventing endogenous flotillin-1 from membrane association significantly reduced bacterial spread. To evaluate whether there was interplay between flotillin-1\u2013 and caveolin-1\u2013mediated endocytosis at L. monocytogenes invagination sites, we measured the area of spread in cells with both caveolin-1 and flotillin-1 knockdown and found a further significant decrease in spread and many cells with complete blockage. This work demonstrates that flotillin-based endocytosis is crucial for cell-to-cell spreading of L. monocytogenes and that this endocytic strategy can internalize large membrane protrusions.<\/jats:p>","DOI":"10.1093\/infdis\/jiaf452","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T11:40:20Z","timestamp":1755862820000},"page":"e778-e788","source":"Crossref","is-referenced-by-count":3,"title":["Role of Flotillin-1 In\n                    <i>Listeria monocytogenes<\/i>\n                    Cell-to-Cell Spreading"],"prefix":"10.1093","volume":"232","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-2349-7039","authenticated-orcid":false,"given":"Petra A","family":"McLeod","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Centre for Cell Biology, Development, and Disease (C2D2), Simon Fraser University , Burnaby, British Columbia ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian A","family":"Guttman","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Centre for Cell Biology, Development, and Disease (C2D2), Simon Fraser University , Burnaby, British Columbia ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,8,26]]},"reference":[{"key":"2025112208025133000_jiaf452-B1","author":"Centers for Disease Control and Prevention","year":"2024"},{"key":"2025112208025133000_jiaf452-B2","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1111\/j.1469-0691.2005.01146.x","article-title":"Understanding how Listeria monocytogenes targets and crosses host barriers","volume":"11","author":"Lecuit","year":"2005","journal-title":"Clin Microbiol Infect"},{"key":"2025112208025133000_jiaf452-B3","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/j.1365-2958.1995.tb02297.x","article-title":"Entry of L. monocytogenes into hepatocytes requires expression 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