{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T09:56:55Z","timestamp":1773395815784,"version":"3.50.1"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:00:00Z","timestamp":1759881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82102404"],"award-info":[{"award-number":["82102404"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82372262"],"award-info":[{"award-number":["82372262"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Noncommunicable Chronic Diseases-National Science and Technology Major Project","award":["2024ZD0529002"],"award-info":[{"award-number":["2024ZD0529002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Klebsiella pneumoniae is a key opportunistic pathogen, and its emerging hypervirulent strains pose a growing public health threat. An association exists between the phospholipid transporter MlaFEDCB and bacterial virulence; however, its regulatory role and underlying mechanisms remain elusive. Herein, we focused on K. pneumoniae virulence regulation via mlaFEDCB under in vitro and in vivo conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>Homology analysis showed that mlaFEDCB gene cluster is highly conservative among gram-negative bacterial strains and is contiguously arranged and co-transcribed within the genome. Experiment involving murine intraperitoneal infection revealed that mice infected with KP-\u0394mlaFEDCB strain showed substantially prolonged survival (P = .0005). Furthermore, transcriptomic analysis showed altered expression of virulence-associated genes, especially fimH and fimD, which are involved in fimbrial structure and host cell adherence. Scanning electron and transmission electron microscopy showed that the WT-KP strain demonstrated a complex fibrous fimbrial network, with several long, thin structures interwoven with those of neighboring bacteria, whereas the KP-\u0394mlaFEDCB strain showed markedly fewer fimbriae and lacked the fimbrial network. Furthermore, bladder epithelial cells adhesion assay showed an apparent reduction in the KP-\u0394mlaFEDCB strain compared with the WT-KP strain. The growth of the KP-\u0394mlaFEDCB strain was significantly compromised in comparison to the wild-type strain under stress conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Thus, mlaFEDCB gene cluster increases the adhesion, invasion, and environmental adaptability of K. pneumoniae by modulating virulence-related gene expression, pilus synthesis, and growth under stress conditions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/infdis\/jiaf517","type":"journal-article","created":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T11:45:40Z","timestamp":1759578340000},"page":"319-327","source":"Crossref","is-referenced-by-count":0,"title":["Phospholipid Transporter MlaFEDCB Regulates\n                    <i>Klebsiella pneumoniae<\/i>\n                    Virulence by Modulating Fimbriae Synthesis and Stress-adaptive Growth"],"prefix":"10.1093","volume":"233","author":[{"given":"Xiaoyu","family":"Zhao","sequence":"first","affiliation":[{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University , Shanghai ,","place":["China"]},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, National Health Commission , Shanghai ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoqi","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University , Shanghai ,","place":["China"]},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, National Health Commission , Shanghai ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2490-1201","authenticated-orcid":false,"given":"Qinglan","family":"Guo","sequence":"additional","affiliation":[{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University , Shanghai ,","place":["China"]},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, National Health Commission , Shanghai ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohan","family":"Ju","sequence":"additional","affiliation":[{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University , Shanghai ,","place":["China"]},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, National Health Commission , Shanghai ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7682-5859","authenticated-orcid":false,"given":"Minggui","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University , Shanghai ,","place":["China"]},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, National Health Commission , Shanghai 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