{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T02:18:19Z","timestamp":1777774699737,"version":"3.51.4"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["T32HL069769"],"award-info":[{"award-number":["T32HL069769"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["T32HL166146"],"award-info":[{"award-number":["T32HL166146"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R35HL145000"],"award-info":[{"award-number":["R35HL145000"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Although manipulation of host hemostasis is a central mechanism in Staphylococcus aureus virulence, clinically relevant phenotypes of S aureus coagulase activity and the variability thereof in the context of bloodstream infections are unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We created a high-throughput staphylothrombin activity and growth fitness assay to characterize fibrin formation and S aureus replication by adding bacterial culture supernatant to human blood plasma. Supernatant is rich in bacterial proteins, including coagulases secreted during growth, but it contains relatively few viable cells. As a result, staphylothrombin activity driven by secreted coagulases can initially be observed and a contribution from bacterial replication can also be captured.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Some S aureus isolates showed slow or absent staphylothrombin activity in a subset of plasma specimens despite robust and rapid fibrin polymerization in other plasmas, suggesting that a host-pathogen match or mismatch determines the observed phenotype. Characterization of S aureus transposon mutant behavior, host plasma coagulation factors, pharmacologic direct thrombin inhibition, and across-host patterns provides clues to complex determinants of coagulase-mediated virulence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Observations of variable staphylothrombin phenotypes may have implications for novel therapeutic approaches and clinical risk stratification in S aureus bloodstream infections.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/infdis\/jiag012","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T12:42:04Z","timestamp":1770122524000},"page":"685-695","source":"Crossref","is-referenced-by-count":0,"title":["Variable Staphylothrombin Activity in\n                    <i>Staphylococcus aureus<\/i>\n                    Bloodstream Infections"],"prefix":"10.1093","volume":"233","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2237-1713","authenticated-orcid":false,"given":"Gregory L","family":"Damhorst","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia ,","place":["USA"]},{"name":"Investigational Clinical Microbiology Core, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madeline J","family":"Boulis","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia ,","place":["USA"]},{"name":"Investigational Clinical Microbiology Core, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2463-576X","authenticated-orcid":false,"given":"Elizabeth B","family":"Nelson","sequence":"additional","affiliation":[{"name":"Wallace H. Coulter Department of Biomedical Engineering, Emory University and Georgia Institute of Technology, Atlanta, Georgia ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0389-2027","authenticated-orcid":false,"given":"Katrina S","family":"Hofstetter","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7457-7606","authenticated-orcid":false,"given":"Jordan","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumiko","family":"Sakurai","sequence":"additional","affiliation":[{"name":"Wallace H. Coulter Department of Biomedical Engineering, Emory University and Georgia Institute of Technology, Atlanta, Georgia ,","place":["USA"]},{"name":"Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jhonny","family":"Correa","sequence":"additional","affiliation":[{"name":"Emory Medical Laboratories , Emory Healthcare, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathaneal","family":"Adam","sequence":"additional","affiliation":[{"name":"Emory Medical Laboratories , Emory Healthcare, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itidal","family":"Reslane","sequence":"additional","affiliation":[{"name":"Vanderbilt University Medical Center, Nashville , Tennessee, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4412-0250","authenticated-orcid":false,"given":"Charlotte G","family":"Muse","sequence":"additional","affiliation":[{"name":"Department of Drug Discovery and Development, Auburn University , Auburn, Alabama, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0141-8807","authenticated-orcid":false,"given":"Peter","family":"Panizzi","sequence":"additional","affiliation":[{"name":"Department of Drug Discovery and Development, Auburn University , Auburn, Alabama, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul D","family":"Fey","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology and Immunology, University of Nebraska Medical Center , Omaha, Nebraska, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janetta","family":"Bryksin","sequence":"additional","affiliation":[{"name":"Emory Medical Laboratories , Emory Healthcare, Atlanta, Georgia, USA"},{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine , Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David N","family":"Alter","sequence":"additional","affiliation":[{"name":"Emory Medical Laboratories , Emory Healthcare, Atlanta, Georgia, USA"},{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine , Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl L","family":"Maier","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine , Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8966-9680","authenticated-orcid":false,"given":"Timothy D","family":"Read","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia ,","place":["USA"]},{"name":"Investigational Clinical Microbiology Core, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah W","family":"Satola","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia ,","place":["USA"]},{"name":"Investigational Clinical Microbiology Core, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0325-7990","authenticated-orcid":false,"given":"Wilbur A","family":"Lam","sequence":"additional","affiliation":[{"name":"Wallace H. 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