{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T02:15:03Z","timestamp":1784081703812,"version":"3.55.0"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Health and Medical Research Council Ideas Grant","award":["GNT 2020606"],"award-info":[{"award-number":["GNT 2020606"]}]},{"name":"Univerity of Melbourne Graduate Research Scholarship"},{"name":"Phyllis Schumann Bequest"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Fc gamma receptor III b (Fc\u03b3RIIIb), a glycosylphosphatidylinositol-linked receptor, is the most abundant neutrophil Fc\u03b3R on neutrophils, followed by Fc\u03b3RIIa. Fc\u03b3Rs interact with IgG, and studies have reported the association of antibody-dependent neutrophil phagocytosis (ADNP) with protection against malaria; however, the role of specific Fc\u03b3Rs is not clear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>To investigate the relative importance of Fc\u03b3RIIIb and Fc\u03b3RIIa as mediators of the ADNP of Plasmodium falciparum\u2013infected erythrocytes, purified neutrophils from healthy donors were treated with tumor necrosis factor (TNF) to mobilize the intracellular stores of Fc\u03b3RIIIb to the surface, followed by enzymatic cleavage of glycosylphosphatidylinositol-linked Fc\u03b3RIIIb with phosphatidylinositol phospholipase C (PIPLC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In TNF\/PIPLC-treated neutrophils, detectable Fc\u03b3RIII decreased by 79% (relative geometric mean fluorescence intensity, 21 \u00b1 4.5 [mean \u00b1 SD]), while Fc\u03b3RIIa detection increased by 82% (182 \u00b1 2.3) as compared with untreated neutrophils (100%). When opsonized infected erythrocytes were incubated with TNF\/PIPLC-treated neutrophils, ADNP by Fc\u03b3RIIIb-depleted neutrophils increased significantly (relative phagocytosis, 585% \u00b1 108%) as compared with untreated neutrophils (100%, P = .042). Using Fc\u03b3R blocking, we show that when compared with no blocker (relative phagocytosis, 100%), ADNP was reduced &amp;gt;5-fold by Fc\u03b3RIIa blocker alone (\u223c17% \u00b1 1.5%, P &amp;lt; .05) and to a similar extent by combined Fc\u03b3RIIa and Fc\u03b3RIII blockers (\u223c24% \u00b1 5.5%, P &amp;lt; .05).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our data suggest that Fc\u03b3RIIa is the main phagocytic receptor that mediates the ADNP of infected erythrocytes and that Fc\u03b3RIIIb acts as a decoy receptor.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/infdis\/jiag071","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T12:33:54Z","timestamp":1770122034000},"page":"e882-e890","source":"Crossref","is-referenced-by-count":1,"title":["Antibody-Dependent Neutrophil Phagocytosis of\n                    <i>Plasmodium falciparum<\/i>\n                    \u2013Infected Erythrocytes Is Mediated by Fc\u03b3RIIa"],"prefix":"10.1093","volume":"233","author":[{"given":"Maria","family":"Saeed","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2677-6208","authenticated-orcid":false,"given":"Elizabeth H","family":"Aitken","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases"},{"name":"Department of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, University of Melbourne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4108-375X","authenticated-orcid":false,"given":"Bruce D","family":"Wines","sequence":"additional","affiliation":[{"name":"Immune Therapies Group, Burnet Institute for Medical Research and Public Health"},{"name":"Department of Immunology, School of Translational 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