{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:01:00Z","timestamp":1790190060326,"version":"4.1.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2018\/15607-1"],"award-info":[{"award-number":["2018\/15607-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/11931-6"],"award-info":[{"award-number":["2017\/11931-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["420616\/2016-0"],"award-info":[{"award-number":["420616\/2016-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["316433\/2021-7"],"award-info":[{"award-number":["316433\/2021-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The majority of new cases of Chagas disease, caused by Trypanosoma cruzi, have been associated with oral infection. Thus, it is reasonable that any vaccine formulation developed should elicit systemic and mucosal immunity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Antigen-specific T-cell, B-cell, antibody-secreting cell, IgG, IgA, cytokine, and parasite DNA were assessed in Balb\/c mice immunized with a recombinant adenovirus replication-deficient vector encoding the parasitic trans-sialidase (rAdTS) and challenged with T. cruzi trypomastigotes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A 2-dose intranasal (IN) rAdTS administration protected mice from lethal subcutaneous and oral parasitic challenges, displaying lower parasitemia and higher survival than control mice. Although intramuscular (IM) rAdTS immunization seemed more immunogenic for CD4+ and CD8+ T cells, IN vaccinees presented the highest responses after parasite exposure. Oral parasitic challenge triggered differential cytokine and cytotoxic transcriptome profiles in the hearts of IM and IN vaccinees. IM and IN rAdTS vaccinations induced high TS-specific IgG and subclass titers in the serum, with predominance of IgG-secreting cells in the spleen and draining lymph nodes. Only IN vaccinees produced TS-specific IgA in mucosal tissues and serum, which showed inverse correlation with parasitemia in vivo. Serum samples from the IN rAdTS vaccinees significantly inhibited parasite invasion in vitro relative to IM counterparts. In addition, IN vaccinees had very low levels of parasite DNA in parasite-reservoir tissues upon oral parasitic challenge relative to IM counterparts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>IN rAdTS vaccination can effectively protect mice against acute Chagas disease caused by lethal distinct T. cruzi challenges, providing an interesting alternative for future vaccine clinical trials.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/infdis\/jiag227","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T11:28:19Z","timestamp":1776770899000},"page":"449-461","source":"Crossref","is-referenced-by-count":0,"title":["Intranasal Vaccination With Recombinant Human Adenovirus Encoding\n                    <i>trans<\/i>\n                    -Sialidase Gene Protects Mice From Lethal Subcutaneous and Oral\n                    <i>Trypanosoma cruzi<\/i>\n                    Challenges"],"prefix":"10.1093","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3676-9749","authenticated-orcid":false,"given":"Isa\u00fa Henrique","family":"Noronha","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology, Federal University of S\u00e3o Paulo , S\u00e3o Paulo, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1755-2904","authenticated-orcid":false,"given":"Liana Nanako","family":"Wada","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Federal University of S\u00e3o Paulo , Santos, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara Santos","family":"Rossetto","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology, Federal University of S\u00e3o Paulo , S\u00e3o Paulo, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara F","family":"Moraschi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology, Federal University of S\u00e3o Paulo , S\u00e3o Paulo, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila P","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology, Federal University of S\u00e3o Paulo , S\u00e3o Paulo, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6301-7219","authenticated-orcid":false,"given":"Jociel Klleyton","family":"Santos Santana","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, S\u00e3o Paulo State University , Araraquara, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4041-3463","authenticated-orcid":false,"given":"Rafael Domingos","family":"do Valle","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Federal University of S\u00e3o Paulo , Santos, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Carlos Gon\u00e7alo Piza","family":"J\u00fanior","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Federal University of S\u00e3o Paulo , Santos, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3232-4766","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Aristeu da Rosa","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, S\u00e3o Paulo State University , Araraquara, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4536-891X","authenticated-orcid":false,"given":"Daniela Santoro","family":"Rosa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology, Federal University of S\u00e3o Paulo , S\u00e3o Paulo, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2847-9407","authenticated-orcid":false,"given":"Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Oxford Vaccine Group, Department of Paediatrics, University of Oxford , Oxford ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4508-0505","authenticated-orcid":false,"given":"Marcela F","family":"Lopes","sequence":"additional","affiliation":[{"name":"Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro , Rio de Janeiro, Rio de Janeiro ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5057-4983","authenticated-orcid":false,"given":"Daniel Araki","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Federal University of S\u00e3o Paulo , Santos, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo T","family":"Gazzinelli","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Massachusetts Medical School , Worcester, Massachusetts ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6780-5397","authenticated-orcid":false,"given":"Karina R","family":"Bortoluci","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Federal University of S\u00e3o Paulo , S\u00e3o Paulo, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3634-2531","authenticated-orcid":false,"given":"Gustavo H G","family":"Trossini","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, School of Pharmaceutical Sciences, University of S\u00e3o Paulo , S\u00e3o Paulo, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8333-2884","authenticated-orcid":false,"given":"Eduardo L V","family":"Silveira","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of S\u00e3o Paulo , S\u00e3o Paulo, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1126-7563","authenticated-orcid":false,"given":"Jos\u00e9 Ronnie C","family":"de 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