{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:13:13Z","timestamp":1788369193235,"version":"build-2803163510"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The attaching and effacing pathogens, enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC), remodel the host actin cytoskeleton to form actin-rich pedestals that anchor the bacteria atop intestinal epithelial cells. Although pedestal formation requires clathrin-endocytic proteins, the role of clathrin-independent proteins like flotillin 1 remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Flotillin 1 localization in pedestal formation was analyzed using immunofluorescence microscopy during EPEC and EHEC infections in HeLa cells. This protein's function was then examined using competing peptide interference and small interfering RNA (siRNA)\u2013mediated knockdown approaches.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Flotillin 1 localizes to the membranes of EPEC pedestals. Disruption of flotillin 1 membrane association or depletion of flotillin 1 using siRNA resulted in significantly elongated EPEC pedestals. A similar pattern of flotillin 1 localization is seen at EHEC pedestals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our work expands current models of attaching and effacing host-pathogen interactions by highlighting a role for flotillin 1 in controlling pedestal length.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/infdis\/jiag314","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T12:29:03Z","timestamp":1781612943000},"page":"256-266","source":"Crossref","is-referenced-by-count":0,"title":["Flotillin 1 Regulation of Enteropathogenic\n                    <i>Escherichia coli<\/i>\n                    Pedestal Length"],"prefix":"10.1093","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-2349-7039","authenticated-orcid":false,"given":"Petra A","family":"McLeod","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Centre for Cell Biology, Development, and Disease (C2D2), Simon Fraser University , Burnaby, British Columbia ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed M H","family":"Bshina","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Centre for Cell Biology, Development, and Disease (C2D2), Simon Fraser University , Burnaby, British Columbia ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter L","family":"Beach","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Centre for Cell Biology, Development, and Disease (C2D2), Simon Fraser University , Burnaby, British Columbia ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian A","family":"Guttman","sequence":"additional","affiliation":[{"name":"Department of 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