{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:07:54Z","timestamp":1789564074173,"version":"build-2803163510"},"reference-count":41,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001691","name":"JSPS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"KAKENHI","doi-asserted-by":"publisher","award":["24K18631"],"award-info":[{"award-number":["24K18631"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"AMED","doi-asserted-by":"publisher","award":["JP266f0137005"],"award-info":[{"award-number":["JP266f0137005"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Disease","doi-asserted-by":"publisher","award":["R21AI186385"],"award-info":[{"award-number":["R21AI186385"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Endogenous human herpesvirus 6 (eHHV-6), in which the entire viral genome is integrated into human chromosomes, is present in approximately 1% of the population and has been associated with various clinical conditions, including neurological disorders. However, its biological significance remains unclear due to the lack of appropriate experimental models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We established a patient-derived induced pluripotent stem cell (iPSC)\u2013based tissue culture model using lymphoblastoid cell lines from individuals with eHHV-6B. iPSCs retaining the integrated viral genome were generated and subsequently differentiated into neural stem cells (NSCs). Viral gene expression was evaluated by RT-qPCR under basal conditions and following chemical stimulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The integrated HHV-6B genome was transcriptionally silent in iPSCs but exhibited spontaneous low-level expression of the immediate-early gene U90 and the late gene U100 in NSCs. Chemical stimulation further enhanced U90 expression, whereas induction of U100 did not reach statistical significance. These findings indicate preferential activation of early viral transcriptional programs in neural lineage cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Neural lineage cells provide a permissive environment for expression of eHHV-6B transcripts. This patient-derived iPSC-based model provides a platform for investigating the biological significance of neural lineage-specific eHHV-6 transcripts and may facilitate interpretation of HHV-6 DNA detection in patients with eHHV-6 by enabling studies of cell type-dependent viral transcription.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/infdis\/jiag444","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T19:46:16Z","timestamp":1788378376000},"source":"Crossref","is-referenced-by-count":0,"title":["A Patient-Derived iPSC-based Model Reveals Neural Lineage-Specific Transcription of Endogenous HHV-6B"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2847-3374","authenticated-orcid":false,"given":"Hiroki","family":"Miura","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Fujita Health University School of Medicine , Toyoake ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2332-9895","authenticated-orcid":false,"given":"Tomoki","family":"Inagaki","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease Research, Fujita Health University School of Medicine , Toyoake ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2697-0194","authenticated-orcid":false,"given":"Tamae","family":"Ohye","sequence":"additional","affiliation":[{"name":"Department of Genetic Counseling, Graduate School of Medical Sciences, Fujita Health University , Aichi, Toyoake ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Fujita Health University School of Medicine , Toyoake ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7228-0839","authenticated-orcid":false,"given":"Takayuki","family":"Murata","sequence":"additional","affiliation":[{"name":"Department of Virology, Fujita Health University School of Medicine , Toyoake ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4125-4536","authenticated-orcid":false,"given":"Naoki","family":"Yahata","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Fujita Health University School of Medicine , Toyoake ,","place":["Japan"]},{"name":"Department of Anatomy I, Fujita Health University School of Medicine , Toyoake ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3942-7098","authenticated-orcid":false,"given":"Yoshiki","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Fujita Health University School of Medicine , Toyoake ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6168-8063","authenticated-orcid":false,"given":"Yotaro","family":"Kondo","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Fujita Health University School of Medicine , Toyoake ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1553-2539","authenticated-orcid":false,"given":"Jun-ichi","family":"Kawada","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Fujita Health University School of Medicine , Toyoake ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1612-7060","authenticated-orcid":false,"given":"Masaru","family":"Ihira","sequence":"additional","affiliation":[{"name":"Faculty of Clinical Science for Biological Monitoring, Fujita Health University School of Health Sciences , Toyoake, Aichi ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1794-9736","authenticated-orcid":false,"given":"Yuki","family":"Higashimoto","sequence":"additional","affiliation":[{"name":"Faculty of Cellular and Molecular Biology, Fujita Health University School of Health Sciences , Toyoake, Aichi ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8541-6413","authenticated-orcid":false,"given":"Yoshitaka","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Virology, Nagoya University 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