{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:51:40Z","timestamp":1791341500079,"version":"4.3.1"},"reference-count":15,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"ATCC"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The ring-stage survival assay (RSA) is a valuable tool for assessing in vitro Plasmodium falciparum tolerance to dihydroartemisinin. However, laboratories with unreliable or no access to premixed commercial gas may not perform the RSA under the recommended physiological normoxia. We measured survival rates of artemisinin (ART)-sensitive and ART-resistant strains in the normoxic commercial gas and hyperoxic candle jar gas. RSA readouts for ART-resistant strains were significantly lower (P &amp;lt; .05) under hyperoxia compared to physiological normoxia, whereas those of ART-sensitive strains were unaltered. These findings have important implications for interlaboratory comparison of RSA data and understanding the mechanisms of resistance to endoperoxide drugs.<\/jats:p>","DOI":"10.1093\/infdis\/jiag490","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T11:52:01Z","timestamp":1790164321000},"source":"Crossref","is-referenced-by-count":0,"title":["Brief Exposure to Hyperoxia is Associated With Reduced\n                    <i>Plasmodium falciparum<\/i>\n                    In Vitro Tolerance to Dihydroartemisinin"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7137-7935","authenticated-orcid":false,"given":"Standwell C","family":"Nkhoma","sequence":"first","affiliation":[{"name":"ATCC, 10801 University Boulevard , Manassas, Virginia ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amel O A","family":"Ahmed","sequence":"additional","affiliation":[{"name":"ATCC, 10801 University Boulevard , Manassas, Virginia ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujatha","family":"Rashid","sequence":"additional","affiliation":[{"name":"ATCC, 10801 University Boulevard , Manassas, Virginia ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4357-8940","authenticated-orcid":false,"given":"Robert E","family":"Molestina","sequence":"additional","affiliation":[{"name":"ATCC, 10801 University Boulevard , Manassas, Virginia ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"2026100622063523300_jiag490-B1","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1056\/NEJMoa0808859","article-title":"Artemisinin resistance in Plasmodium falciparum malaria","volume":"361","author":"Dondorp","year":"2009","journal-title":"N Engl J Med"},{"key":"2026100622063523300_jiag490-B2","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/nature12876","article-title":"A molecular marker of artemisinin-resistant Plasmodium falciparum malaria","volume":"505","author":"Ariey","year":"2014","journal-title":"Nature"},{"key":"2026100622063523300_jiag490-B3","doi-asserted-by":"crossref","first-page":"12799","DOI":"10.1073\/pnas.1812317115","article-title":"Mutations in Plasmodium falciparum actin-binding protein coronin confer reduced artemisinin susceptibility","volume":"115","author":"Demas","year":"2018","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026100622063523300_jiag490-B4","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/ng.3189","article-title":"Genetic architecture of artemisinin-resistant Plasmodium falciparum","volume":"47","author":"Miotto","year":"2015","journal-title":"Nat Genet"},{"key":"2026100622063523300_jiag490-B5","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1038\/nature14412","article-title":"A molecular mechanism of artemisinin resistance in Plasmodium falciparum malaria","volume":"520","author":"Mbengue","year":"2015","journal-title":"Nature"},{"key":"2026100622063523300_jiag490-B6","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1016\/S1473-3099(13)70252-4","article-title":"Novel phenotypic assays for the detection of artemisinin-resistant Plasmodium falciparum malaria in Cambodia: in-vitro and ex-vivo drug-response studies","volume":"13","author":"Witkowski","year":"2013","journal-title":"Lancet Infect Dis"},{"key":"2026100622063523300_jiag490-B7","doi-asserted-by":"crossref","first-page":"900","DOI":"10.3390\/pathogens12070900","article-title":"Cultivation of asexual intraerythrocytic stages of Plasmodium falciparum","volume":"12","author":"Basco","year":"2023","journal-title":"Pathogens"},{"key":"2026100622063523300_jiag490-B8","doi-asserted-by":"crossref","first-page":"e0128221","DOI":"10.1128\/spectrum.01282-21","article-title":"Naturally acquired Kelch13 mutations in Plasmodium falciparum strains modulate in vitro ring-stage artemisinin-based drug tolerance and parasite survival in response to hyperoxia","volume":"10","author":"Duffy","year":"2022","journal-title":"Microbiol Spectr"},{"key":"2026100622063523300_jiag490-B9","doi-asserted-by":"crossref","first-page":"e1012514","DOI":"10.1371\/journal.ppat.1012514","article-title":"Understanding the significance of oxygen tension on the biology of Plasmodium falciparum blood stages: from the human body to the laboratory","volume":"20","author":"Nahid","year":"2024","journal-title":"PLoS Pathog"},{"key":"2026100622063523300_jiag490-B10","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1126\/science.1260867","article-title":"Drug resistance. K13-propeller mutations confer artemisinin resistance in Plasmodium falciparum clinical isolates","volume":"347","author":"Straimer","year":"2015","journal-title":"Science"},{"key":"2026100622063523300_jiag490-B11","doi-asserted-by":"crossref","first-page":"e1006930","DOI":"10.1371\/journal.ppat.1006930","article-title":"Oxidative stress and protein damage responses mediate artemisinin resistance in malaria parasites","volume":"14","author":"Rocamora","year":"2018","journal-title":"PLoS Pathog"},{"key":"2026100622063523300_jiag490-B12","doi-asserted-by":"crossref","first-page":"2917","DOI":"10.1016\/j.celrep.2019.10.095","article-title":"Decreased K13 abundance reduces hemoglobin catabolism and proteotoxic stress, underpinning artemisinin resistance","volume":"29","author":"Yang","year":"2019","journal-title":"Cell Rep"},{"key":"2026100622063523300_jiag490-B13","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1039\/b110817b","article-title":"Alkylation of heme by the antimalarial drug artemisinin","volume":"38","author":"Robert","year":"2002","journal-title":"Chem Commun"},{"key":"2026100622063523300_jiag490-B14","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1038\/s41467-020-20805-w","article-title":"Artemisinin-resistant K13 mutations rewire Plasmodium falciparum's intra-erythrocytic metabolic program to enhance survival","volume":"12","author":"Mok","year":"2021","journal-title":"Nat Commun"},{"key":"2026100622063523300_jiag490-B15","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1038\/s42003-025-09057-w","article-title":"PfPI3K inhibition reversed artemisinin resistance by increasing ROS and ubiquitinated protein levels in Plasmodium falciparum","volume":"8","author":"Shi","year":"2025","journal-title":"Commun Biol"}],"container-title":["The Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jid\/advance-article-pdf\/doi\/10.1093\/infdis\/jiag490\/71383493\/jiag490.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jid\/advance-article-pdf\/doi\/10.1093\/infdis\/jiag490\/71383493\/jiag490.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:06:44Z","timestamp":1791338804000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jid\/advance-article\/doi\/10.1093\/infdis\/jiag490\/8833991"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,24]]},"references-count":15,"URL":"https:\/\/doi.org\/10.1093\/infdis\/jiag490","relation":{},"ISSN":["0022-1899","1537-6613"],"issn-type":[{"value":"0022-1899","type":"print"},{"value":"1537-6613","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,24]]},"article-number":"jiag490"}}