{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:47:30Z","timestamp":1791308850803,"version":"4.3.1"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,1,1]]},"DOI":"10.1093\/infdis\/jiq017","type":"journal-article","created":{"date-parts":[[2010,12,8]],"date-time":"2010-12-08T04:05:02Z","timestamp":1291781102000},"page":"95-102","source":"Crossref","is-referenced-by-count":101,"title":["Multivalent Smallpox DNA Vaccine Delivered by Intradermal Electroporation Drives Protective Immunity in Nonhuman Primates Against Lethal Monkeypox Challenge"],"prefix":"10.1093","volume":"203","author":[{"given":"Lauren A.","family":"Hirao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruxandra","family":"Draghia-Akli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan T.","family":"Prigge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhishek","family":"Satishchandran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Hammarlund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir S.","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahar","family":"Babas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lowrey","family":"Rhodes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Silvera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Slifka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niranjan Y.","family":"Sardesai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David B.","family":"Weiner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,1,1]]},"reference":[{"key":"key\n\t\t\t\t20170702133423_bib1","first-page":"593","article-title":"A human infection caused by monkeypox virus in Basankusu Territory, Democratic Republic of the Congo","volume":"46","author":"Ladnyj","year":"1972","journal-title":"Bull World Health Organ."},{"key":"key\n\t\t\t\t20170702133423_bib2","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1016\/j.vaccine.2006.10.047","article-title":"Safety, immunogenicity and efficacy of modified vaccinia Ankara (MVA) against Dryvax challenge in vaccinia-naive and vaccinia-immune individuals","volume":"25","author":"Parrino","year":"2007","journal-title":"Vaccine."},{"key":"key\n\t\t\t\t20170702133423_bib3","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1016\/j.vaccine.2009.11.030","article-title":"A randomized, double-blind, dose-finding Phase II study to evaluate immunogenicity and safety of the third generation smallpox vaccine candidate IMVAMUNE","volume":"28","author":"von Krempelhuber","year":"2010","journal-title":"Vaccine"},{"key":"key\n\t\t\t\t20170702133423_bib4","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/s00281-006-0046-z","article-title":"DNA vaccines for HIV: challenges and opportunities","volume":"28","author":"Hokey","year":"2006","journal-title":"Springer Semin Immunopathol."},{"key":"key\n\t\t\t\t20170702133423_bib5","doi-asserted-by":"crossref","first-page":"4433","DOI":"10.1128\/JVI.78.9.4433-4443.2004","article-title":"Smallpox DNA vaccine protects nonhuman primates against lethal monkeypox","volume":"78","author":"Hooper","year":"2004","journal-title":"J Virol."},{"key":"key\n\t\t\t\t20170702133423_bib6","doi-asserted-by":"crossref","first-page":"3517","DOI":"10.1128\/JVI.01854-07","article-title":"Vaccination of BALB\/c mice with Escherichia coli-expressed vaccinia virus proteins A27L, B5R, and D8L protects mice from lethal vaccinia virus challenge","volume":"82","author":"Berhanu","year":"2008","journal-title":"J Virol."},{"key":"key\n\t\t\t\t20170702133423_bib7","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1038\/nature02331","article-title":"Immunogenicity of a highly attenuated MVA smallpox vaccine and protection against monkeypox","volume":"428","author":"Earl","year":"2004","journal-title":"Nature."},{"key":"key\n\t\t\t\t20170702133423_bib8","doi-asserted-by":"crossref","first-page":"2552","DOI":"10.4049\/jimmunol.177.4.2552","article-title":"Subunit recombinant vaccine protects against monkeypox","volume":"177","author":"Heraud","year":"2006","journal-title":"J Immunol."},{"key":"key\n\t\t\t\t20170702133423_bib9","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0042-6822(02)00038-7","article-title":"Four-gene-combination DNA vaccine protects mice against a lethal vaccinia virus challenge and elicits appropriate antibody responses in nonhuman primates","volume":"306","author":"Hooper","year":"2003","journal-title":"Virology."},{"issue":"pt 4","key":"key\n\t\t\t\t20170702133423_bib10","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1099\/0022-1317-79-4-877","article-title":"Intracellular and extracellular vaccinia virions enter cells by different mechanisms","volume":"79","author":"Vanderplasschen","year":"1998","journal-title":"J Gen Virol."},{"key":"key\n\t\t\t\t20170702133423_bib11","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1038\/nm1457","article-title":"Quantification of antibody responses against multiple antigens of the two infectious forms of Vaccinia virus provides a benchmark for smallpox vaccination","volume":"12","author":"Putz","year":"2006","journal-title":"Nat Med."},{"key":"key\n\t\t\t\t20170702133423_bib12","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1128\/JVI.56.2.482-488.1985","article-title":"Isolation and characterization of neutralizing monoclonal antibodies to vaccinia virus","volume":"56","author":"Rodriguez","year":"1985","journal-title":"J Virol."},{"key":"key\n\t\t\t\t20170702133423_bib13","doi-asserted-by":"crossref","first-page":"9455","DOI":"10.1128\/JVI.01149-06","article-title":"Vaccinia virus F9 virion membrane protein is required for entry but not virus assembly, in contrast to the related L1 protein","volume":"80","author":"Brown","year":"2006","journal-title":"J Virol."},{"key":"key\n\t\t\t\t20170702133423_bib14","doi-asserted-by":"crossref","first-page":"3353","DOI":"10.1128\/JVI.74.7.3353-3365.2000","article-title":"Vaccinia virus envelope H3L protein binds to cell surface heparan sulfate and is important for intracellular mature virion morphogenesis and virus infection in vitro and in vivo","volume":"74","author":"Lin","year":"2000","journal-title":"J Virol."},{"key":"key\n\t\t\t\t20170702133423_bib15","doi-asserted-by":"crossref","first-page":"11724","DOI":"10.1128\/JVI.79.18.11724-11733.2005","article-title":"Vaccinia virus H3L envelope protein is a major target of neutralizing antibodies in humans and elicits protection against lethal challenge in mice","volume":"79","author":"Davies","year":"2005","journal-title":"J Virol."},{"key":"key\n\t\t\t\t20170702133423_bib16","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1006\/viro.1995.1378","article-title":"A myristylated membrane protein encoded by the vaccinia virus L1R open reading frame is the target of potent neutralizing monoclonal antibodies","volume":"211","author":"Wolffe","year":"1995","journal-title":"Virology."},{"key":"key\n\t\t\t\t20170702133423_bib17","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/0042-6822(90)90422-N","article-title":"Hemadsorption and fusion inhibition activities of hemagglutinin analyzed by vaccinia virus mutants","volume":"175","author":"Seki","year":"1990","journal-title":"Virology."},{"key":"key\n\t\t\t\t20170702133423_bib18","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1006\/viro.1999.0096","article-title":"DNA vaccination with vaccinia virus L1R and A33R genes protects mice against a lethal poxvirus challenge","volume":"266","author":"Hooper","year":"2000","journal-title":"Virology"},{"key":"key\n\t\t\t\t20170702133423_bib19","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1006\/viro.1998.9516","article-title":"Neutralizing and protective antibodies directed against vaccinia virus envelope antigens","volume":"254","author":"Galmiche","year":"1999","journal-title":"Virology."},{"key":"key\n\t\t\t\t20170702133423_bib20","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1038\/nm1261","article-title":"Smallpox vaccine-induced antibodies are necessary and sufficient for protection against monkeypox virus","volume":"11","author":"Edghill-Smith","year":"2005","journal-title":"Nat Med."},{"key":"key\n\t\t\t\t20170702133423_bib21","doi-asserted-by":"crossref","first-page":"6333","DOI":"10.1128\/JVI.00115-06","article-title":"Protective immunity against secondary poxvirus infection is dependent on antibody but not on CD4 or CD8 T-cell function","volume":"80","author":"Panchanathan","year":"2006","journal-title":"J Virol"},{"key":"key\n\t\t\t\t20170702133423_bib22","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.bbrc.2007.11.052","article-title":"Persistent antibody and T cell responses induced by HIV-1 DNA vaccine delivered by electroporation","volume":"366","author":"Cristillo","year":"2008","journal-title":"Biochem Biophys Res Commun."},{"key":"key\n\t\t\t\t20170702133423_bib23","doi-asserted-by":"crossref","first-page":"2278","DOI":"10.4049\/jimmunol.158.5.2278","article-title":"Different T helper cell types and antibody isotypes generated by saline and gene gun DNA immunization","volume":"158","author":"Feltquate","year":"1997","journal-title":"J Immunol."},{"key":"key\n\t\t\t\t20170702133423_bib24","doi-asserted-by":"crossref","first-page":"4607","DOI":"10.1016\/j.vaccine.2005.08.049","article-title":"Successful parallel development and integration of a plasmid-based biologic, container\/closure system and electrokinetic delivery device","volume":"24","author":"Hebel","year":"2006","journal-title":"Vaccine."},{"key":"key\n\t\t\t\t20170702133423_bib25","doi-asserted-by":"crossref","first-page":"3112","DOI":"10.1016\/j.vaccine.2008.02.036","article-title":"Combined effects of IL-12 and electroporation enhances the potency of DNA vaccination in macaques","volume":"26","author":"Hirao","year":"2008","journal-title":"Vaccine."},{"key":"key\n\t\t\t\t20170702133423_bib26","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1111\/j.1600-0684.2005.00124.x","article-title":"SIV DNA vaccine co-administered with IL-12 expression plasmid enhances CD8 SIV cellular immune responses in cynomolgus macaques","volume":"34","author":"Boyer","year":"2005","journal-title":"J Med Primatol."},{"key":"key\n\t\t\t\t20170702133423_bib27","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.vaccine.2009.09.133","article-title":"Molecular smallpox vaccine delivered by alphavirus replicons elicits protective immunity in mice and non-human primates","volume":"28","author":"Hooper","year":"2009","journal-title":"Vaccine."},{"key":"key\n\t\t\t\t20170702133423_bib28","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.virol.2007.04.010","article-title":"DNA\/MVA HIV-1\/AIDS vaccine elicits long-lived vaccinia virus-specific immunity and confers protection against a lethal monkeypox challenge","volume":"366","author":"Nigam","year":"2007","journal-title":"Virology."},{"issue":"5","key":"key\n\t\t\t\t20170702133423_bib29","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1128\/AAC.01596-08","article-title":"ST-246(R) antiviral efficacy in a non-human primate monkeypox model: determination of the minimal effective dose and human dose justification","volume":"53","author":"Jordan","year":"2009","journal-title":"Antimicrob Agents Chemother."},{"key":"key\n\t\t\t\t20170702133423_bib30","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1002\/rmv.571","article-title":"New generation smallpox vaccines: a review of preclinical and clinical data","volume":"18","author":"Andrew","year":"2008","journal-title":"Rev Med Virol."},{"key":"key\n\t\t\t\t20170702133423_bib31","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1016\/j.vaccine.2006.11.017","article-title":"Smallpox DNA vaccine delivered by novel skin electroporation device protects mice against intranasal poxvirus challenge","volume":"25","author":"Hooper","year":"2007","journal-title":"Vaccine."},{"key":"key\n\t\t\t\t20170702133423_bib32","doi-asserted-by":"crossref","first-page":"4461","DOI":"10.1016\/j.vaccine.2005.08.010","article-title":"Efficacy of novel plasmid DNA encoding vaccinia antigens in improving current smallpox vaccination strategy","volume":"24","author":"Otero","year":"2006","journal-title":"Vaccine."},{"key":"key\n\t\t\t\t20170702133423_bib33","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1128\/JVI.66.1.386-398.1992","article-title":"Identification and characterization of vaccinia virus genes encoding proteins that are highly antigenic in animals and are immunodominant in vaccinated humans","volume":"66","author":"Demkowicz","year":"1992","journal-title":"J Virol."},{"key":"key\n\t\t\t\t20170702133423_bib34","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1086\/512161","article-title":"Immunological memory after exposure to variola virus, monkeypox virus, and vaccinia virus","volume":"195","author":"Sivapalasingam","year":"2007","journal-title":"J Infect Dis."},{"key":"key\n\t\t\t\t20170702133423_bib35","doi-asserted-by":"crossref","first-page":"2787","DOI":"10.1016\/j.vaccine.2006.12.037","article-title":"Adjuvant-enhanced antibody responses to recombinant proteins correlates with protection of mice and monkeys to orthopoxvirus challenges","volume":"25","author":"Fogg","year":"2007","journal-title":"Vaccine."},{"key":"key\n\t\t\t\t20170702133423_bib36","doi-asserted-by":"crossref","first-page":"3507","DOI":"10.1016\/j.vaccine.2008.04.017","article-title":"Targeting the vaccinia virus L1 protein to the cell surface enhances production of neutralizing antibodies","volume":"26","author":"Golden","year":"2008","journal-title":"Vaccine."},{"key":"key\n\t\t\t\t20170702133423_bib37","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/1743-422X-6-28","article-title":"Engineering the vaccinia virus L1 protein for increased neutralizing antibody response after DNA immunization","volume":"6","author":"Shinoda","year":"2009","journal-title":"Virol J."},{"key":"key\n\t\t\t\t20170702133423_bib38","doi-asserted-by":"crossref","first-page":"4969","DOI":"10.4049\/jimmunol.171.10.4969","article-title":"Cutting edge: long-term B cell memory in humans after smallpox vaccination","volume":"171","author":"Crotty","year":"2003","journal-title":"J Immunol."}],"container-title":["The Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jid\/article-pdf\/203\/1\/95\/18063865\/jiq017.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,4]],"date-time":"2023-06-04T12:09:37Z","timestamp":1685880577000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jid\/article-lookup\/doi\/10.1093\/infdis\/jiq017"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1,1]]},"references-count":38,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2011,1,1]]},"published-print":{"date-parts":[[2011,1,1]]}},"URL":"https:\/\/doi.org\/10.1093\/infdis\/jiq017","relation":{},"ISSN":["1537-6613","0022-1899"],"issn-type":[{"value":"1537-6613","type":"electronic"},{"value":"0022-1899","type":"print"}],"subject":[],"published":{"date-parts":[[2011,1,1]]}}}