{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T21:02:00Z","timestamp":1767906120015,"version":"3.49.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"4","funder":[{"name":"Intramural Research Program of the US National Institutes of Health Clinical Center"},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,19]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glucan is the major cell wall component of Pneumocystis cysts. In the current study, we have characterized Pneumocystis Bgl2 (EC 3.2.1.58), an enzyme with glucanosyltransferase and \u03b2-1,3 endoglucanase activity in other fungi. Pneumocystis murina, Pneumocystis carinii, and Pneumocystis jirovecii bgl2 complementary DNA sequences encode proteins of 437, 447, and 408 amino acids, respectively. Recombinant P. murina Bgl2 expressed in COS-1 cells demonstrated \u03b2-glucanase activity, as shown by degradation of the cell wall of Pneumocystis cysts. It also cleaved reduced laminaripentaose and transferred oligosaccharides, resulting in polymers of 6 and 7 glucan residues, demonstrating glucanosyltransferase activity. Surprisingly, confocal immunofluorescence analysis of P. murina\u2013infected mouse lung sections using an antibody against recombinant Bgl2 showed that the native protein is localized primarily to the trophic form of Pneumocystis in both untreated mice and mice treated with caspofungin, an antifungal drug that inhibits \u03b2-1,3-glucan synthase. Thus, like other fungi, Bgl2 of Pneumocystis has both endoglucanase and glucanosyltransferase activities. Given that it is expressed primarily in trophic forms, further studies are needed to better understand its role in the biology of Pneumocystis.<\/jats:p>","DOI":"10.1093\/infdis\/jiz172","type":"journal-article","created":{"date-parts":[[2019,4,13]],"date-time":"2019-04-13T19:11:29Z","timestamp":1555182689000},"page":"657-665","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of Pneumocystis murina Bgl2, an Endo-\u03b2-1,3-Glucanase and Glucanosyltransferase"],"prefix":"10.1093","volume":"220","author":[{"given":"Geetha","family":"Kutty","sequence":"first","affiliation":[{"name":"Critical Care Medicine Department, National Institutes of Health Clinical Center, Bethesda, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A Sally","family":"Davis","sequence":"additional","affiliation":[{"name":"Diagnostic Medicine\/Pathobiology, Kansas State University College of Veterinary Medicine, Manhattan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaitlynn","family":"Schuck","sequence":"additional","affiliation":[{"name":"Diagnostic Medicine\/Pathobiology, Kansas State University College of Veterinary Medicine, Manhattan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mya","family":"Masterson","sequence":"additional","affiliation":[{"name":"Diagnostic Medicine\/Pathobiology, Kansas State University College of Veterinary Medicine, Manhattan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Honghui","family":"Wang","sequence":"additional","affiliation":[{"name":"Critical Care Medicine Department, National Institutes of Health Clinical Center, Bethesda, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yueqin","family":"Liu","sequence":"additional","affiliation":[{"name":"Critical Care Medicine Department, National Institutes of Health Clinical Center, Bethesda, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5191-9880","authenticated-orcid":false,"given":"Joseph A","family":"Kovacs","sequence":"additional","affiliation":[{"name":"Critical Care Medicine Department, National Institutes of Health Clinical Center, Bethesda, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,4,18]]},"reference":[{"key":"2020010308574886600_CIT0001","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1056\/NEJM198112103052402","article-title":"An outbreak of community-acquired Pneumocystis carinii pneumonia: initial manifestation of cellular immune dysfunction","volume":"305","author":"Masur","year":"1981","journal-title":"N Engl J Med"},{"key":"2020010308574886600_CIT0002","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1093\/oxfordjournals.aje.a116691","article-title":"Trends in the incidence of outcomes defining acquired immunodeficiency syndrome (AIDS) in the Multicenter AIDS Cohort Study: 1985\u20131991","volume":"137","author":"Munoz","year":"1993","journal-title":"Am J Epidemiol"},{"key":"2020010308574886600_CIT0003","doi-asserted-by":"crossref","first-page":"83","DOI":"10.7326\/0003-4819-80-1-83","article-title":"Pneumocystis carinii pneumonia in the United States. 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