{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T16:54:46Z","timestamp":1770137686775,"version":"3.49.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2019,4,23]],"date-time":"2019-04-23T00:00:00Z","timestamp":1555977600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P20GM113123"],"award-info":[{"award-number":["P20GM113123"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Center of Biomedical Research Excellence","award":["5P20GM113123"],"award-info":[{"award-number":["5P20GM113123"]}]},{"name":"IDeA Network of Biomedical research Excellence","award":["5P20GM103442"],"award-info":[{"award-number":["5P20GM103442"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We sought to determine the role of host interleukin 17A (IL-17A) response against colonizing Streptococcus pneumoniae, and its transition to a pathogen during coinfection with an influenza virus, influenza A H1N1 A\/Puerto Rico\/8\/1934 (PR8).<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Wild-type (WT) C57BL\/6 mice were intranasally inoculated with S. pneumoniae serotype 6A to establish colonization and later infected with the influenza strain, PR8, resulting in invasive S. pneumoniae disease. The role of the IL-17A response in colonization and coinfection was investigated in WT, RoR\u03b3t\u2212\/\u2212 and RAG1\u2212\/\u2212 mice with antibody-mediated depletion of IL-17A (WT) and CD90 cells (RAG1\u2212\/\u2212).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RAG1\u2212\/\u2212 mice did not clear colonization and IL-17A neutralization impaired 6A clearance in WT mice. RoR\u03b3t\u2212\/\u2212 mice also had reduced clearance. S. pneumoniae\u2013PR8 coinfection elicited a robust IL-17A response in the nasopharynx; IL-17A neutralization reduced S. pneumoniae invasive disease. RoR\u03b3t\u2212\/\u2212 mice also had reduced S. pneumoniae disease in a coinfection model. Depletion of CD90+ cells suppressed the IL-17A response and reduced S. pneumoniae invasion in RAG1\u2212\/\u2212 mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data show that although IL-17A reduces S. pneumoniae colonization, coinfection with influenza virus elicits a robust innate IL-17A response that promotes inflammation and S. pneumoniae disease in the nasopharynx.<\/jats:p><\/jats:sec>","DOI":"10.1093\/infdis\/jiz193","type":"journal-article","created":{"date-parts":[[2019,4,17]],"date-time":"2019-04-17T19:12:41Z","timestamp":1555528361000},"page":"902-912","source":"Crossref","is-referenced-by-count":15,"title":["Double-Edged Role of Interleukin 17A in Streptococcus pneumoniae Pathogenesis During Influenza Virus Coinfection"],"prefix":"10.1093","volume":"220","author":[{"given":"Ganesh","family":"Ambigapathy","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taylor","family":"Schmit","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ram Kumar","family":"Mathur","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Albany Medical College"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suba","family":"Nookala","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saad","family":"Bahri","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand 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