{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:36:11Z","timestamp":1758126971885,"version":"3.37.3"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T00:00:00Z","timestamp":1566345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571180","81873760"],"award-info":[{"award-number":["81571180","81873760"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,11,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Streptococcus pneumonia meningitis (PM) is a major cause of childhood neurological deficits. Although the Notch1 signaling pathway regulates neurogenesis and neuroinflammation, we know little about its expression or influence on hippocampal neurogenesis and gliogenesis during PM.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We used immunofluorescence and Western blots to detect Notch1 signaling expression during experimental PM. Through double-labeling immunofluorescence, we investigated proliferation and differentiation in the dentate gyrus (DG) in PM before and after treatment with exogenous Notch1 activator (Jagged1) and inhibitor (IMR-1).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Our results showed that Notch1 was activated after 24 hours in PM. Compared with the phosphate-buffered saline (PBS) control, Jagged1 increased the proliferation of neural stem cells and progenitor cells (NS\/PCs) in DG. After 14 and 28 days of meningitis, astrocyte differentiation increased compared with control. Astrocyte differentiation was higher in the Jagged1 versus the PBS group. In contrast, IMR-1 increased neuronal differentiation but decreased astrocyte differentiation compared with dimethyl sulfoxide treatment.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Under PM, Notch1 signaling promotes NS\/PC proliferation and astrocyte differentiation in DG, while decreasing neuronal differentiation. Transient activation of the Notch1 signaling pathway explains the reactive gliogenesis and limited neuronal differentiation observed in PM.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiz414","type":"journal-article","created":{"date-parts":[[2019,8,15]],"date-time":"2019-08-15T15:10:04Z","timestamp":1565881804000},"page":"1977-1988","source":"Crossref","is-referenced-by-count":5,"title":["Notch1 Signaling Pathway Promotes Proliferation and Mediates Differentiation Direction in Hippocampus of Streptococcus pneumonia Meningitis Rats"],"prefix":"10.1093","volume":"220","author":[{"given":"Zhijie","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pediatric Neurology, Xinhua Hospital affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danfeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Neurology, Xinhua Hospital affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengnan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pediatric Neurology, Xinhua Hospital affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di","family":"Lian","sequence":"additional","affiliation":[{"name":"Department of Pediatric Neurology, Xinhua Hospital affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Neurology, Xinhua Hospital affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dake","family":"He","sequence":"additional","affiliation":[{"name":"Department of Pediatric Neurology, Xinhua Hospital affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pediatric Neurology, Xinhua Hospital affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,8,21]]},"reference":[{"key":"2019110610561801500_CIT0001","doi-asserted-by":"crossref","first-page":"S37","DOI":"10.1016\/j.cmi.2016.01.007","article-title":"ESCMID guideline: diagnosis and treatment of acute bacterial meningitis","volume":"22","author":"van de 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