{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T15:31:45Z","timestamp":1767713505016,"version":"3.41.2"},"reference-count":37,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2019,9,6]],"date-time":"2019-09-06T00:00:00Z","timestamp":1567728000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin","doi-asserted-by":"publisher","award":["17JCQNJC09300"],"award-info":[{"award-number":["17JCQNJC09300"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31800125"],"award-info":[{"award-number":["31800125"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The human intestinal pathogen enterohemorrhagic Escherichia coli (EHEC) O157:H7 causes bloody diarrhea, hemorrhagic colitis, and fatal hemolytic uremic syndrome. Its genome contains 177 unique O islands (OIs), which contribute largely to the high virulence and pathogenicity although most OI genes remain uncharacterized. In the current study, we demonstrated that OI-19 is required for EHEC O157:H7 adherence to host cells. Z0442 (OI-encoded virulence regulator A [OvrA]) encoded in OI-19 positively regulated bacterial adherence by activating locus of enterocyte effacement (LEE) gene expression through direct OvrA binding to the gene promoter region of the LEE gene master regulator Ler. Mouse colonization experiments revealed that OvrA promotes EHEC O157:H7 adherence in mouse intestine, preferentially the colon. Finally, OvrA also regulated virulence in other non-O157 pathogenic E. coli, including EHEC strains O145:H28 and O157:H16 and enteropathogenic E. coli strain O55:H7. Our work markedly enriches the understanding of bacterial adherence control and provides another example of laterally acquired regulators that mediate LEE gene expression.<\/jats:p>","DOI":"10.1093\/infdis\/jiz458","type":"journal-article","created":{"date-parts":[[2019,9,5]],"date-time":"2019-09-05T11:41:12Z","timestamp":1567683672000},"source":"Crossref","is-referenced-by-count":5,"title":["Transcriptional Activator OvrA Encoded in O Island 19 Modulates Virulence Gene Expression in Enterohemorrhagic Escherichia coli O157:H7"],"prefix":"10.1093","author":[{"given":"Bin","family":"Liu","sequence":"first","affiliation":[{"name":"TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junyue","family":"Wang","sequence":"additional","affiliation":[{"name":"TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Ding","sequence":"additional","affiliation":[{"name":"TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pan","family":"Yang","sequence":"additional","affiliation":[{"name":"TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Yang","sequence":"additional","affiliation":[{"name":"TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China"},{"name":"The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,9,6]]},"reference":[{"key":"2019102003090235700_CIT0001","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4014\/jmb.0908.08007","article-title":"A brief overview of Escherichia coli O157:H7 and its plasmid O157","volume":"20","author":"Lim","year":"2010","journal-title":"J Microbiol Biotechnol"},{"key":"2019102003090235700_CIT0002","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/j.mcna.2013.04.001","article-title":"Enterohemorrhagic Escherichia coli infections and the hemolytic-uremic syndrome","volume":"97","author":"Page","year":"2013","journal-title":"Med Clin North Am"},{"key":"2019102003090235700_CIT0003","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/nrmicro818","article-title":"Pathogenic Escherichia coli","volume":"2","author":"Kaper","year":"2004","journal-title":"Nat Rev Microbiol"},{"key":"2019102003090235700_CIT0004","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1155\/2002\/410980","article-title":"Enteropathogenic and enterohemorrhagic Escherichia coli infections: emerging themes in pathogenesis and prevention","volume":"16","author":"Vallance","year":"2002","journal-title":"Can J Gastroenterol"},{"key":"2019102003090235700_CIT0005","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1128\/IAI.73.5.2573-2585.2005","article-title":"Enteropathogenic and enterohemorrhagic Escherichia coli infections: translocation, translocation, translocation","volume":"73","author":"Garmendia","year":"2005","journal-title":"Infect Immun"},{"key":"2019102003090235700_CIT0006","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1073\/pnas.92.5.1664","article-title":"A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens","volume":"92","author":"McDaniel","year":"1995","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2019102003090235700_CIT0007","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1046\/j.1365-2958.1998.01144.x","article-title":"Enteropathogenic and enterohaemorrhagic Escherichia coli: more subversive elements","volume":"30","author":"Frankel","year":"1998","journal-title":"Mol Microbiol"},{"key":"2019102003090235700_CIT0008","doi-asserted-by":"crossref","first-page":"4199","DOI":"10.1128\/IAI.01927-06","article-title":"Enteropathogenic and enterohemorrhagic Escherichia coli virulence gene regulation","volume":"75","author":"Mellies","year":"2007","journal-title":"Infect Immun"},{"key":"2019102003090235700_CIT0009","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/nrmicro.2017.20","article-title":"Assembly, structure, function and regulation of type III secretion systems","volume":"15","author":"Deng","year":"2017","journal-title":"Nat Rev Microbiol"},{"key":"2019102003090235700_CIT0010","doi-asserted-by":"crossref","first-page":"e8094","DOI":"10.1371\/journal.pone.0008094","article-title":"The association of virulence factors with genomic islands","volume":"4","author":"Ho Sui","year":"2009","journal-title":"PLoS One"},{"key":"2019102003090235700_CIT0011","doi-asserted-by":"crossref","first-page":"3469","DOI":"10.1128\/JB.01717-08","article-title":"Genomic islands of uropathogenic Escherichia coli contribute to virulence","volume":"191","author":"Lloyd","year":"2009","journal-title":"J Bacteriol"},{"key":"2019102003090235700_CIT0012","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/j.1574-6968.2009.01686.x","article-title":"Genome-wide identification of novel genomic islands that contribute to Salmonella virulence in mouse systemic infection","volume":"297","author":"Haneda","year":"2009","journal-title":"FEMS Microbiol Lett"},{"key":"2019102003090235700_CIT0013","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/35054089","article-title":"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7","volume":"409","author":"Perna","year":"2001","journal-title":"Nature"},{"key":"2019102003090235700_CIT0014","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/dnares\/8.1.11","article-title":"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12","volume":"8","author":"Hayashi","year":"2001","journal-title":"DNA Res"},{"key":"2019102003090235700_CIT0015","doi-asserted-by":"crossref","first-page":"4975","DOI":"10.1128\/AEM.00297-09","article-title":"Adherence of Escherichia coli O157:H7 mutants in vitro and in ligated pig intestines","volume":"75","author":"Yin","year":"2009","journal-title":"Appl Environ Microbiol"},{"key":"2019102003090235700_CIT0016","doi-asserted-by":"crossref","first-page":"5779","DOI":"10.1128\/AEM.00507-09","article-title":"Contributions of O island 48 to adherence of enterohemorrhagic Escherichia coli O157:H7 to epithelial cells in vitro and in ligated pig ileal loops","volume":"75","author":"Yin","year":"2009","journal-title":"Appl Environ Microbiol"},{"key":"2019102003090235700_CIT0017","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1111\/j.1365-2958.2011.07650.x","article-title":"Transcriptional regulators of the GAD acid stress island are carried by effector protein-encoding prophages and indirectly control type III secretion in enterohemorrhagic Escherichia coli O157:H7","volume":"80","author":"Tree","year":"2011","journal-title":"Mol Microbiol"},{"key":"2019102003090235700_CIT0018","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1111\/j.1365-2958.2011.07927.x","article-title":"Identification of a novel prophage regulator in Escherichia coli controlling the expression of type III secretion","volume":"83","author":"Flockhart","year":"2012","journal-title":"Mol Microbiol"},{"key":"2019102003090235700_CIT0019","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1046\/j.1365-2958.2003.03911.x","article-title":"Identification and characterization of NleA, a non-LEE-encoded type III translocated virulence factor of enterohaemorrhagic Escherichia coli O157:H7","volume":"51","author":"Gruenheid","year":"2004","journal-title":"Mol Microbiol"},{"key":"2019102003090235700_CIT0020","doi-asserted-by":"crossref","first-page":"8411","DOI":"10.1128\/IAI.73.12.8411-8417.2005","article-title":"Characterization of two non-locus of enterocyte effacement-encoded type III-translocated effectors, NleC and NleD, in attaching and effacing pathogens","volume":"73","author":"March\u00e9s","year":"2005","journal-title":"Infect Immun"},{"key":"2019102003090235700_CIT0021","doi-asserted-by":"crossref","first-page":"4930","DOI":"10.1128\/JCM.41.11.4930-4940.2003","article-title":"Association of genomic O island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and\/or serious disease","volume":"41","author":"Karmali","year":"2003","journal-title":"J Clin Microbiol"},{"key":"2019102003090235700_CIT0022","volume-title":"Guide for the care and use of laboratory animals","author":"National Research Council.","year":"2011","edition":"8th ed."},{"key":"2019102003090235700_CIT0023","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products","volume":"97","author":"Datsenko","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2019102003090235700_CIT0024","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1471-2199-4-11","article-title":"Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli","volume":"4","author":"Murphy","year":"2003","journal-title":"BMC Mol Biol"},{"key":"2019102003090235700_CIT0025","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1099\/0022-1317-50-9-759","article-title":"An in-vitro model for studying the interaction of Escherichia coli O157:H7 and other enteropathogens with bovine primary cell cultures","volume":"50","author":"Dibb-Fuller","year":"2001","journal-title":"J Med Microbiol"},{"key":"2019102003090235700_CIT0026","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1111\/j.1574-6968.2007.00633.x","article-title":"Evaluation of housekeeping genes in Listeria monocytogenes as potential internal control references for normalizing mRNA expression levels in stress adaptation models using real-time PCR","volume":"269","author":"Tasara","year":"2007","journal-title":"FEMS Microbiol Lett"},{"key":"2019102003090235700_CIT0027","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2019102003090235700_CIT0028","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1128\/IAI.57.4.1290-1298.1989","article-title":"Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli","volume":"57","author":"Knutton","year":"1989","journal-title":"Infect Immun"},{"key":"2019102003090235700_CIT0029","doi-asserted-by":"crossref","first-page":"2950","DOI":"10.1128\/AEM.68.6.2950-2958.2002","article-title":"Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation","volume":"68","author":"Djordjevic","year":"2002","journal-title":"Appl Environ Microbiol"},{"key":"2019102003090235700_CIT0030","doi-asserted-by":"crossref","first-page":"6592","DOI":"10.1038\/ncomms7592","article-title":"Enterohemorrhagic Escherichia coli senses low biotin status in the large intestine for colonization and infection","volume":"6","author":"Yang","year":"2015","journal-title":"Nat Commun"},{"key":"2019102003090235700_CIT0031","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.semcdb.2015.10.032","article-title":"The flagellum in bacterial pathogens: For motility and a whole lot more","volume":"46","author":"Chaban","year":"2015","journal-title":"Semin Cell Dev Biol"},{"key":"2019102003090235700_CIT0032","doi-asserted-by":"crossref","first-page":"313","DOI":"10.4103\/0019-5359.55113","article-title":"Bacterial biofilm formation, pathogenicity, diagnostics and control: An overview","volume":"63","author":"Sawhney","year":"2009","journal-title":"Indian J Med Sci"},{"key":"2019102003090235700_CIT0033","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1128\/CMR.11.1.142","article-title":"Diarrheagenic Escherichia coli","volume":"11","author":"Nataro","year":"1998","journal-title":"Clin Microbiol Rev"},{"key":"2019102003090235700_CIT0034","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1146\/annurev.micro.54.1.641","article-title":"Pathogenicity islands and the evolution of microbes","volume":"54","author":"Hacker","year":"2000","journal-title":"Annu Rev Microbiol"},{"key":"2019102003090235700_CIT0035","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1128\/CMR.16.3.365-378.2003","article-title":"Virulence of enteropathogenic Escherichia coli, a global pathogen","volume":"16","author":"Clarke","year":"2003","journal-title":"Clin Microbiol Rev"},{"key":"2019102003090235700_CIT0036","doi-asserted-by":"crossref","first-page":"315","DOI":"10.4161\/viru.24318","article-title":"Modulation of the enterohemorrhagic E. coli virulence program through the human gastrointestinal tract","volume":"4","author":"Barnett Foster","year":"2013","journal-title":"Virulence"},{"key":"2019102003090235700_CIT0037","doi-asserted-by":"crossref","first-page":"2606","DOI":"10.1128\/IAI.65.7.2606-2612.1997","article-title":"Enteropathogenic Escherichia coli protein secretion is induced in response to conditions similar to those in the gastrointestinal tract","volume":"65","author":"Kenny","year":"1997","journal-title":"Infect Immun"}],"container-title":["The Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jid\/advance-article-pdf\/doi\/10.1093\/infdis\/jiz458\/30247159\/jiz458.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jid\/advance-article-pdf\/doi\/10.1093\/infdis\/jiz458\/30247159\/jiz458.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T09:27:57Z","timestamp":1610962077000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jid\/advance-article\/doi\/10.1093\/infdis\/jiz458\/5564344"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,6]]},"references-count":37,"URL":"https:\/\/doi.org\/10.1093\/infdis\/jiz458","relation":{},"ISSN":["0022-1899","1537-6613"],"issn-type":[{"type":"print","value":"0022-1899"},{"type":"electronic","value":"1537-6613"}],"subject":[],"published":{"date-parts":[[2019,9,6]]},"article-number":"jiz458"}}