{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T17:01:05Z","timestamp":1776704465611,"version":"3.51.2"},"reference-count":27,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2019,9,21]],"date-time":"2019-09-21T00:00:00Z","timestamp":1569024000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32 AI118690"],"award-info":[{"award-number":["T32 AI118690"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K24 HL093294-06"],"award-info":[{"award-number":["K24 HL093294-06"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K23 AI114844-02"],"award-info":[{"award-number":["K23 AI114844-02"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["CA15074"],"award-info":[{"award-number":["CA15074"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Seattle Children's Center for Clinical and Translational Research Clinical Research Scholar's Program"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hematopoietic cell transplant (HCT) recipients are frequently infected with respiratory viruses (RVs) in the upper respiratory tract (URT), but the concordance between URT and lower respiratory tract (LRT) RV detection is not well characterized.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Hematopoietic cell transplant candidates and recipients with respiratory symptoms and LRT and URT RV testing via multiplex PCR from 2009 to 2016 were included. Logistic regression models were used to analyze risk factors for LRT RV detection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two-hundred thirty-five HCT candidates or recipients had URT and LRT RV testing within 3 days. Among 115 subjects (49%) positive for a RV, 37% (42 of 115) had discordant sample pairs. Forty percent (17 of 42) of discordant pairs were positive in the LRT but negative in the URT. Discordance was common for adenovirus (100%), metapneumovirus (44%), rhinovirus (34%), and parainfluenza virus type 3 (28%); respiratory syncytial virus was highly concordant (92%). Likelihood of LRT detection was increased with URT detection (oods ratio [OR] = 73.7; 95% confidence interval [CI], 26.7\u2013204) and in cytomegalovirus-positive recipients (OR = 3.70; 95% CI, 1.30\u201310.0).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>High rates of discordance were observed for certain RVs. Bronchoalveolar lavage sampling may provide useful diagnostic information to guide management in symptomatic HCT candidates and recipients.<\/jats:p><\/jats:sec>","DOI":"10.1093\/infdis\/jiz470","type":"journal-article","created":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T19:25:16Z","timestamp":1568229916000},"source":"Crossref","is-referenced-by-count":9,"title":["Predictive Value of Respiratory Viral Detection in the Upper Respiratory Tract for Infection of the Lower Respiratory Tract With Hematopoietic Stem Cell Transplantation"],"prefix":"10.1093","author":[{"given":"Jim","family":"Boonyaratanakornkit","sequence":"first","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, Seattle, Washington, USA"},{"name":"Department of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meghana","family":"Vivek","sequence":"additional","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hu","family":"Xie","sequence":"additional","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven A","family":"Pergam","sequence":"additional","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, Seattle, Washington, USA"},{"name":"Department of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang-Shing","family":"Cheng","sequence":"additional","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, Seattle, Washington, USA"},{"name":"Department of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Mielcarek","sequence":"additional","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, Seattle, Washington, USA"},{"name":"Department of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua A","family":"Hill","sequence":"additional","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, Seattle, Washington, USA"},{"name":"Department of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith R","family":"Jerome","sequence":"additional","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, Seattle, Washington, USA"},{"name":"Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5350-9025","authenticated-orcid":false,"given":"Ajit P","family":"Limaye","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy","family":"Leisenring","sequence":"additional","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J","family":"Boeckh","sequence":"additional","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, Seattle, Washington, USA"},{"name":"Department of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alpana","family":"Waghmare","sequence":"additional","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, Seattle, Washington, USA"},{"name":"Department of Pediatrics, University of Washington, Seattle, Washington, USA"},{"name":"Seattle Children's Hospital, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,9,21]]},"reference":[{"key":"2019101706185557400_CIT0001","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1111\/j.1365-2141.2008.07295.x","article-title":"The challenge of respiratory virus infections in hematopoietic cell transplant recipients","volume":"143","author":"Boeckh","year":"2008","journal-title":"Br J Haematol"},{"key":"2019101706185557400_CIT0002","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1016\/j.bbmt.2013.05.005","article-title":"Mortality rates of human metapneumovirus and respiratory syncytial virus lower respiratory tract infections in hematopoietic cell transplantation recipients","volume":"19","author":"Renaud","year":"2013","journal-title":"Biol Blood Marrow Transplant"},{"key":"2019101706185557400_CIT0003","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.1016\/j.bbmt.2015.06.015","article-title":"Significant transplantation-related mortality from respiratory virus infections within the first one hundred days in children after hematopoietic stem cell transplantation","volume":"21","author":"Hutspardol","year":"2015","journal-title":"Biol Blood Marrow Transplant"},{"key":"2019101706185557400_CIT0004","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/j.jcv.2013.09.016","article-title":"Respiratory virus detection in nasopharyngeal aspirate versus bronchoalveolar lavage is dependent on virus type in children with chronic respiratory symptoms","volume":"58","author":"Wurzel","year":"2013","journal-title":"J Clin Virol"},{"key":"2019101706185557400_CIT0005","doi-asserted-by":"crossref","first-page":"4020","DOI":"10.1128\/JCM.01944-14","article-title":"Predictive value of testing nasopharyngeal samples for respiratory viruses in the setting of lower respiratory tract disease","volume":"52","author":"Hakki","year":"2014","journal-title":"J Clin Microbiol"},{"key":"2019101706185557400_CIT0006","doi-asserted-by":"crossref","first-page":"3784","DOI":"10.1128\/JCM.01516-15","article-title":"FilmArray respiratory panel assay: comparison of nasopharyngeal swabs and bronchoalveolar lavage samples","volume":"53","author":"Azadeh","year":"2015","journal-title":"J Clin Microbiol"},{"key":"2019101706185557400_CIT0007","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1086\/653529","article-title":"Upper and lower respiratory tract viral infections and acute graft rejection in lung transplant recipients","volume":"51","author":"Soccal","year":"2010","journal-title":"Clin Infect Dis"},{"key":"2019101706185557400_CIT0008","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1111\/j.1399-3062.2009.00400.x","article-title":"Comparison of conventional and molecular detection of respiratory viruses in hematopoietic cell transplant recipients","volume":"11","author":"Kuypers","year":"2009","journal-title":"Transpl Infect Dis"},{"key":"2019101706185557400_CIT0009","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/1471-2334-8-73","article-title":"Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid","volume":"8","author":"Khot","year":"2008","journal-title":"BMC Infect Dis"},{"key":"2019101706185557400_CIT0010","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1128\/AEM.02383-08","article-title":"Sequencing and analysis of fungal rRNA operons for development of broad-range fungal PCR assays","volume":"75","author":"Khot","year":"2009","journal-title":"Appl Environ Microbiol"},{"key":"2019101706185557400_CIT0011","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1164\/rccm.2007-413ST","article-title":"An official American Thoracic Society research statement: noninfectious lung injury after hematopoietic stem cell transplantation: idiopathic pneumonia syndrome","volume":"183","author":"Panoskaltsis-Mortari","year":"2011","journal-title":"Am J Respir Crit Care Med"},{"key":"2019101706185557400_CIT0012","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1128\/JCM.34.7.1649-1653.1996","article-title":"Rapid diagnosis of respiratory syncytial virus infections in immunocompromised adults","volume":"34","author":"Englund","year":"1996","journal-title":"J Clin Microbiol"},{"key":"2019101706185557400_CIT0013","doi-asserted-by":"crossref","first-page":"2685723","DOI":"10.1155\/2018\/2685723","article-title":"Comparison of respiratory pathogen detection in upper versus lower respiratory tract samples using the biofire FilmArray respiratory panel in the immunocompromised host","volume":"2018","author":"Azadeh","year":"2018","journal-title":"Can Respir J"},{"key":"2019101706185557400_CIT0014","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1111\/resp.13049","article-title":"Nasopharyngeal viral PCR in immunosuppressed patients and its association with virus detection in bronchoalveolar lavage by PCR","volume":"22","author":"Lachant","year":"2017","journal-title":"Respirology"},{"key":"2019101706185557400_CIT0015","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.bbmt.2018.08.021","article-title":"Risk factors for parainfluenza virus lower respiratory tract disease after hematopoietic cell transplantation","volume":"25","author":"Seo","year":"2019","journal-title":"Biol Blood Marrow Transplant"},{"key":"2019101706185557400_CIT0016","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1093\/infdis\/jix390","article-title":"Supplemental oxygen-free days in hematopoietic cell transplant recipients with respiratory syncytial virus","volume":"216","author":"Waghmare","year":"2017","journal-title":"J Infect Dis"},{"key":"2019101706185557400_CIT0017","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1164\/rccm.201002-0221OC","article-title":"Viral load drives disease in humans experimentally infected with respiratory syncytial virus","volume":"182","author":"DeVincenzo","year":"2010","journal-title":"Am J Respir Crit Care Med"},{"key":"2019101706185557400_CIT0018","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1002\/jmv.21548","article-title":"Correlation of rhinovirus load in the respiratory tract and clinical symptoms in hospitalized immunocompetent and immunocompromised patients","volume":"81","author":"Gerna","year":"2009","journal-title":"J Med Virol"},{"key":"2019101706185557400_CIT0019","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1086\/651662","article-title":"Respiratory virus pneumonia after hematopoietic cell transplantation (HCT): associations between viral load in bronchoalveolar lavage samples, viral RNA detection in serum samples, and clinical outcomes of HCT","volume":"201","author":"Campbell","year":"2010","journal-title":"J Infect Dis"},{"key":"2019101706185557400_CIT0020","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1182\/blood-2006-12-060343","article-title":"Respiratory virus infection among hematopoietic cell transplant recipients: evidence for asymptomatic parainfluenza virus infection","volume":"110","author":"Peck","year":"2007","journal-title":"Blood"},{"key":"2019101706185557400_CIT0021","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1016\/j.bbmt.2018.12.005","article-title":"Human rhinovirus infections in hematopoietic cell transplant recipients: risk score for progression to lower respiratory tract infection","volume":"25","author":"Waghmare","year":"2019","journal-title":"Biol Blood Marrow Transplant"},{"key":"2019101706185557400_CIT0022","doi-asserted-by":"crossref","first-page":"53930","DOI":"10.18632\/oncotarget.10182","article-title":"Virus infection facilitates the development of severe pneumonia in transplant patients with hematologic malignancies","volume":"7","author":"Yue","year":"2016","journal-title":"Oncotarget"},{"key":"2019101706185557400_CIT0023","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1111\/j.1399-3062.2010.00504.x","article-title":"Pre-transplant cytomegalovirus (CMV) serostatus remains the most important determinant of CMV reactivation after allogeneic hematopoietic stem cell transplantation in the era of surveillance and preemptive therapy","volume":"12","author":"George","year":"2010","journal-title":"Transpl Infect Dis"},{"key":"2019101706185557400_CIT0024","doi-asserted-by":"crossref","first-page":"5711","DOI":"10.1182\/blood-2008-10-143560","article-title":"How we treat cytomegalovirus in hematopoietic cell transplant recipients","volume":"113","author":"Boeckh","year":"2009","journal-title":"Blood"},{"key":"2019101706185557400_CIT0025","doi-asserted-by":"crossref","first-page":"2240","DOI":"10.1182\/blood.V95.7.2240","article-title":"Increased transplant-related morbidity and mortality in CMV-seropositive patients despite highly effective prevention of CMV disease after allogeneic T-cell-depleted stem cell transplantation","volume":"95","author":"Broers","year":"2000","journal-title":"Blood"},{"key":"2019101706185557400_CIT0026","doi-asserted-by":"crossref","first-page":"3881","DOI":"10.4049\/jimmunol.1800171","article-title":"Cytomegalovirus: shape-shifting the immune system","volume":"200","author":"Picarda","year":"2018","journal-title":"J Immunol"},{"key":"2019101706185557400_CIT0027","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1093\/cid\/civ272","article-title":"Clinical outcomes associated with respiratory virus detection before allogeneic hematopoietic stem cell transplant","volume":"61","author":"Campbell","year":"2015","journal-title":"Clin Infect Dis"}],"container-title":["The Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jid\/advance-article-pdf\/doi\/10.1093\/infdis\/jiz470\/30159440\/jiz470.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jid\/advance-article-pdf\/doi\/10.1093\/infdis\/jiz470\/30159440\/jiz470.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T08:02:34Z","timestamp":1721721754000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jid\/advance-article\/doi\/10.1093\/infdis\/jiz470\/5572451"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,21]]},"references-count":27,"URL":"https:\/\/doi.org\/10.1093\/infdis\/jiz470","relation":{},"ISSN":["0022-1899","1537-6613"],"issn-type":[{"value":"0022-1899","type":"print"},{"value":"1537-6613","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,21]]},"article-number":"jiz470"}}