{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:03:35Z","timestamp":1753877015368,"version":"3.41.2"},"reference-count":40,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100006567","name":"Suhara Memorial Foundation","doi-asserted-by":"publisher","award":["JP19fk0108047"],"award-info":[{"award-number":["JP19fk0108047"]}],"id":[{"id":"10.13039\/501100006567","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science KAKENHI","doi-asserted-by":"crossref","award":["26670204"],"award-info":[{"award-number":["26670204"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Research Center for Protozoan Diseases"},{"name":"Obihiro University of Agriculture and Veterinary Medicine","award":["27-joint-6","28-joint-3","29-joint-4"],"award-info":[{"award-number":["27-joint-6","28-joint-3","29-joint-4"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Toxoplasmosis, a parasitic disease caused by Toxoplasma gondii, is an important cause of miscarriage or adverse fetal effects, including neurological and ocular manifestations in humans. Current anti-Toxoplasma drugs have limited efficacy against toxoplasmosis and also have severe side effects. Therefore, novel efficacious drugs are urgently needed. Here, we identified metacytofilin (MCF) from a fungal Metarhizium species as a potential anti-Toxoplasma compound.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Anti-Toxoplasma activities of MCF and its derivatives were evaluated in vitro and in vivo using nonpregnant and pregnant mice. To understand the mode of action of MCF, the RNA expression of host and parasite genes was investigated by RNAseq.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In vitro, MCF inhibited the viability of intracellular and extracellular T. gondii. Administering MCF intraperitoneally or orally to mice after infection with T. gondii tachyzoites increased mouse survival compared with the untreated animals. Remarkably, oral administration of MCF to pregnant mice prevented vertical transmission of the parasite. Interestingly, RNA sequencing of T. gondii\u2013infected cells treated with MCF showed that MCF inhibited DNA replication and enhanced RNA degradation in the parasites.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>With its potent anti\u2013T. gondii activity, MCF is a strong candidate for future drug development against toxoplasmosis.<\/jats:p><\/jats:sec>","DOI":"10.1093\/infdis\/jiz501","type":"journal-article","created":{"date-parts":[[2019,9,28]],"date-time":"2019-09-28T18:16:10Z","timestamp":1569694570000},"source":"Crossref","is-referenced-by-count":2,"title":["Metacytofilin Is a Potent Therapeutic Drug Candidate for Toxoplasmosis"],"prefix":"10.1093","author":[{"given":"Arpron","family":"Leesombun","sequence":"first","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan"},{"name":"Department of Pre-clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Salaya, Phutthamonthon Nakhonpathom, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatomi","family":"Iijima","sequence":"additional","affiliation":[{"name":"Institute of Microbial Chemistry, Shizuoka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kousuke","family":"Umeda","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Kondoh","sequence":"additional","affiliation":[{"name":"Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baldorj","family":"Pagmadulam","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed M","family":"Abdou","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan"},{"name":"Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, South Valley University, Qena City, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7559-5139","authenticated-orcid":false,"given":"Yutaka","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Graduate School of Frontier Science, University of Tokyo, Kashiwa, Chiba, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shun-Ichi","family":"Ohba","sequence":"additional","affiliation":[{"name":"Institute of Microbial Chemistry, Shizuoka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunio","family":"Isshiki","sequence":"additional","affiliation":[{"name":"Institute of Microbial Chemistry, Shinagawa, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyuki","family":"Kimura","sequence":"additional","affiliation":[{"name":"Institute of Microbial Chemistry, Shinagawa, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumiko","family":"Kubota","sequence":"additional","affiliation":[{"name":"Institute of Microbial Chemistry, Shinagawa, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuichi","family":"Sawa","sequence":"additional","affiliation":[{"name":"Institute of Microbial Chemistry, Shinagawa, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Coh-Ichi","family":"Nihei","sequence":"additional","affiliation":[{"name":"Institute of Microbial 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