{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T01:59:28Z","timestamp":1793325568048,"version":"4.5.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2019,10,29]],"date-time":"2019-10-29T00:00:00Z","timestamp":1572307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["PO1 AG032958"],"award-info":[{"award-number":["PO1 AG032958"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 NS093716"],"award-info":[{"award-number":["R01 NS093716"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fisher Family Foundation"},{"name":"Rocky Mountain Neurological Disorders Core","award":["P30NS048154"],"award-info":[{"award-number":["P30NS048154"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,3,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Herpes zoster is linked to amyloid-associated diseases, including dementia, macular degeneration, and diabetes mellitus, in epidemiological studies. Thus, we examined whether varicella-zoster virus (VZV)-infected cells produce amyloid.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Production of intracellular amyloidogenic proteins (amylin, amyloid precursor protein [APP], and amyloid-\u03b2 [A\u03b2]) and amyloid, as well as extracellular amylin, A\u03b2, and amyloid, was compared between mock- and VZV-infected quiescent primary human spinal astrocytes (qHA-sps). The ability of supernatant from infected cells to induce amylin or A\u03b242 aggregation was quantitated. Finally, the amyloidogenic activity of viral peptides was examined.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>VZV-infected qHA-sps, but not mock-infected qHA-sps, contained intracellular amylin, APP, and\/or A\u03b2, and amyloid. No differences in extracellular amylin, A\u03b240, or A\u03b242 were detected, yet only supernatant from VZV-infected cells induced amylin aggregation and, to a lesser extent, A\u03b242 aggregation into amyloid fibrils. VZV glycoprotein B (gB) peptides assembled into fibrils and catalyzed amylin and A\u03b242 aggregation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>VZV-infected qHA-sps produced intracellular amyloid and their extracellular environment promoted aggregation of cellular peptides into amyloid fibrils that may be due, in part, to VZV gB peptides. These findings suggest that together with host and other environmental factors, VZV infection may increase the toxic amyloid burden and contribute to amyloid-associated disease progression.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiz560","type":"journal-article","created":{"date-parts":[[2019,10,24]],"date-time":"2019-10-24T03:28:04Z","timestamp":1571887684000},"page":"1088-1097","source":"Crossref","is-referenced-by-count":57,"title":["Varicella-Zoster Virus Infection of Primary Human Spinal Astrocytes Produces Intracellular Amylin, Amyloid-\u03b2, and an Amyloidogenic Extracellular Environment"],"prefix":"10.1093","volume":"221","author":[{"given":"Andrew N","family":"Bubak","sequence":"first","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina N","family":"Como","sequence":"first","affiliation":[{"name":"Department of 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Medicine, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"James E","family":"Hassell","sequence":"first","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Mescher","sequence":"first","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christy S","family":"Niemeyer","sequence":"first","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi","family":"Mahalingam","sequence":"first","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randall J","family":"Cohrs","sequence":"first","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"},{"name":"Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy D","family":"Boyd","sequence":"first","affiliation":[{"name":"Department of Neurology, University of Colorado School of Medicine, Aurora, Colorado, USA"},{"name":"Rocky Mountain Alzheimer\u2019s Disease Center, University of Colorado School of Medicine, Aurora, Colorado, USA"},{"name":"Linda Crnic Institute for Down Syndrome Research, University of Colorado School of Medicine, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huntington","family":"Potter","sequence":"first","affiliation":[{"name":"Department of Neurology, University of 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