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Although both gene products can function as potential porins, only BB0405 is essential for infection. Here we show that, despite sequence homology and coexpression from the same operon, both proteins differ in their membrane localization attributes, antibody accessibility, and immunogenicity in mice. BB0406 is required for spirochete survival in mammalian hosts, particularly for the disseminated infection in distant organs. We identified that BB0406 interacts with laminin, one of the major constituents of the vascular basement membrane, and facilitates spirochete transmigration across host endothelial cell barriers. A better understanding of how B. burgdorferi transmigrates through dermal and tissue vascular barriers and establishes disseminated infections will contribute to the development of novel therapeutics to combat early infection.<\/jats:p>","DOI":"10.1093\/infdis\/jiz626","type":"journal-article","created":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T04:23:33Z","timestamp":1574396613000},"page":"1438-1447","source":"Crossref","is-referenced-by-count":11,"title":["A Novel Laminin-Binding Protein Mediates Microbial-Endothelial Cell Interactions and Facilitates Dissemination of Lyme Disease Pathogens"],"prefix":"10.1093","volume":"221","author":[{"given":"Sandhya","family":"Bista","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Preeti","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, University 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Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York, USA"},{"name":"Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, New York, USA"},{"name":"Department of Biology and Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Zhnag","sequence":"first","affiliation":[{"name":"Oral and Craniofacial Molecular Biology, School of Dentistry, Virginia Commonwealth University, Richmond, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darrin R","family":"Akins","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Hritzo","sequence":"first","affiliation":[{"name":"Department of Pathology, Uniformed Services 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