{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:05:18Z","timestamp":1758924318821,"version":"3.37.3"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T00:00:00Z","timestamp":1574812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Marc and Lynne Benioff","award":["R21AI28697","R21AI129534","R21EY028318"],"award-info":[{"award-number":["R21AI28697","R21AI129534","R21EY028318"]}]},{"DOI":"10.13039\/501100006506","name":"Brazilian Ministry of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006506","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"name":"UK\u2019s Department for International Development","award":["205377\/Z\/16\/Z"],"award-info":[{"award-number":["205377\/Z\/16\/Z"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation Programme"},{"name":"ZikaPLAN","award":["734584"],"award-info":[{"award-number":["734584"]}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Program for Research Incentives (PIP)\/Fernandes Figueira Institute\/FIOCRUZ"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>There are limited data on the natural history of antenatal Zika virus (ZIKV) exposure in twin pregnancies, especially regarding intertwin concordance of prenatal, placental, and infant outcomes.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>This prospective cohort study included twin pregnancies referred to a single institution from September 2015 to June 2016 with maternal ZIKV. Polymerase chain reaction (PCR) testing of maternal, placental, and neonatal samples was performed. Prenatal ultrasounds were completed for each twin, and histomorphologic analysis was performed for each placenta. Abnormal neonatal outcome was defined as abnormal exam and\/or abnormal imaging. Two- to three-year follow-up of infants included physical exams, neuroimaging, and Bayley-III developmental assessment.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Among 244 pregnancies, 4 twin gestations without coinfection were identified. Zika virus infection occurred at 16\u201333 weeks gestation. Zika virus PCR testing revealed discordance between dichorionic twins, between placentas in a dichorionic pair, between portions of a monochorionic placenta, and between a neonate and its associated placenta. Of the 8 infants, 3 (38%) had an abnormal neonatal outcome. Of 6 infants with long-term follow-up, 3 (50%) have demonstrated ZIKV-related abnormalities.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Neonatal PCR testing, placental findings, and infant outcomes can be discordant between co-twins with antenatal ZIKV exposure. These findings demonstrate that each twin should be evaluated independently for vertical transmission.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/infdis\/jiz629","type":"journal-article","created":{"date-parts":[[2019,11,25]],"date-time":"2019-11-25T20:11:33Z","timestamp":1574712693000},"page":"1838-1845","source":"Crossref","is-referenced-by-count":11,"title":["Discordant Zika Virus Findings in Twin Pregnancies Complicated by Antenatal Zika Virus Exposure: A Prospective Cohort"],"prefix":"10.1093","volume":"221","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2856-3185","authenticated-orcid":false,"given":"Nasim C","family":"Sobhani","sequence":"first","affiliation":[{"name":"Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology, & Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elyzabeth","family":"Avvad-Portari","sequence":"first","affiliation":[{"name":"Fiocruz, Fernandes Figueira Institute, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline C M","family":"Nascimento","sequence":"first","affiliation":[{"name":"Fiocruz, Fernandes Figueira Institute, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heloisa N","family":"Machado","sequence":"first","affiliation":[{"name":"Fiocruz, Fernandes Figueira Institute, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel S S","family":"Lobato","sequence":"first","affiliation":[{"name":"Fiocruz, Fernandes Figueira Institute, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose Paulo","family":"Pereira","sequence":"first","affiliation":[{"name":"Fiocruz, Fernandes Figueira Institute, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikaela S","family":"Esquivel","sequence":"first","affiliation":[{"name":"Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology, & Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zilton C","family":"Vasconcelos","sequence":"first","affiliation":[{"name":"Fiocruz, Fernandes Figueira Institute, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea A","family":"Zin","sequence":"first","affiliation":[{"name":"Fiocruz, Fernandes Figueira Institute, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irena","family":"Tsui","sequence":"first","affiliation":[{"name":"Jules Stein Eye Institute, Retina Division, University of California, Los Angeles, Los Angeles, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina","family":"Adachi","sequence":"first","affiliation":[{"name":"Division of Pediatric Infectious Diseases, Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth B","family":"Brickley","sequence":"first","affiliation":[{"name":"Department of Infectious Disease Epidemiology, London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan J","family":"Fisher","sequence":"first","affiliation":[{"name":"Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology, & Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Nielsen-Saines","sequence":"first","affiliation":[{"name":"Division of Pediatric Infectious Diseases, Department of Pediatrics, University of California, Los Angeles, Los Angeles, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Brasil","sequence":"first","affiliation":[{"name":"Fiocruz, Fernandes Figueira Institute, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria E","family":"Moreira","sequence":"first","affiliation":[{"name":"Fiocruz, Fernandes Figueira Institute, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0891-6964","authenticated-orcid":false,"given":"Stephanie L","family":"Gaw","sequence":"first","affiliation":[{"name":"Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology, & Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,11,27]]},"reference":[{"key":"2020051111581129700_CIT0001","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.1056\/NEJMoa1602412","article-title":"Zika virus infection in pregnant women in Rio de Janeiro","volume":"375","author":"Brasil","year":"2016","journal-title":"N Engl J Med"},{"key":"2020051111581129700_CIT0002","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1001\/jamaneurol.2016.3720","article-title":"Congenital Zika virus infection: beyond neonatal microcephaly","volume":"73","author":"Melo","year":"2016","journal-title":"JAMA Neurol"},{"key":"2020051111581129700_CIT0003","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1056\/NEJMsr1604338","article-title":"Zika virus and birth defects\u2013reviewing the evidence for causality","volume":"374","author":"Rasmussen","year":"2016","journal-title":"N Engl J Med"},{"key":"2020051111581129700_CIT0004","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1007\/s00401-017-1699-5","article-title":"The spectrum of neuropathological changes associated with congenital Zika virus infection","volume":"133","author":"Chimelli","year":"2017","journal-title":"Acta Neuropathol"},{"key":"2020051111581129700_CIT0005","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1177\/0022034517723325","article-title":"Postnatal identification of Zika virus peptides from saliva","volume":"96","author":"Zuanazzi","year":"2017","journal-title":"J Dent Res"},{"key":"2020051111581129700_CIT0006","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1590\/0004-282x20170066","article-title":"Discordant clinical outcomes of congenital Zika virus infection in twin pregnancies","volume":"75","author":"Linden","year":"2017","journal-title":"Arq Neuropsiquiatr"},{"key":"2020051111581129700_CIT0007","first-page":"1","article-title":"Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells","volume":"9","author":"Caires-Junior","year":"2018","journal-title":"Nat Commun"},{"key":"2020051111581129700_CIT0008","doi-asserted-by":"crossref","first-page":"2377","DOI":"10.1056\/NEJMc1800098","article-title":"Neurodevelopment in infants exposed to Zika virus in utero","volume":"379","author":"Lopes Moreira","year":"2018","journal-title":"N Engl J Med"},{"key":"2020051111581129700_CIT0009","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1038\/s41591-019-0496-1","article-title":"Delayed childhood neurodevelopment and neurosensory alterations in the second year of life in a prospective cohort of ZIKV-exposed children","volume":"25","author":"Nielsen-Saines","year":"2019","journal-title":"Nat Med"},{"key":"2020051111581129700_CIT0010","doi-asserted-by":"crossref","first-page":"698","DOI":"10.5858\/arpa.2015-0225-CC","article-title":"Sampling and definitions of placental lesions: Amsterdam Placental Workshop Group Consensus Statement","volume":"140","author":"Khong","year":"2016","journal-title":"Arch Pathol Lab Med"},{"key":"2020051111581129700_CIT0011"},{"key":"2020051111581129700_CIT0012","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1590\/1982-43272664201606","article-title":"Bayley","volume":"26","author":"Madaschi","year":"2016","journal-title":"Paied\u00e9ia (Ribeir\u00e3 Preto)"},{"key":"2020051111581129700_CIT0013","first-page":"1631760","article-title":"Use of the Bayley scales of infant and toddler development, third edition, to assess developmental outcome in infants and young children in an urban setting in South Africa","volume":"2017","author":"Ballot","year":"2017","journal-title":"Int Sch Res Notices"},{"key":"2020051111581129700_CIT0014","doi-asserted-by":"crossref","first-page":"379","DOI":"10.2307\/1126464","article-title":"Comparisons of mental and motor test scores for ages 1-15 months by sex, birth order, race, geographical location, and education of parents","volume":"36","author":"Bayley","year":"1965","journal-title":"Child Dev"},{"key":"2020051111581129700_CIT0015","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1375\/twin.9.3.456","article-title":"Discordant fetal infection for parvovirus B19 in a dichorionic twin pregnancy","volume":"9","author":"Dickinson","year":"2006","journal-title":"Twin Res Hum Genet"},{"key":"2020051111581129700_CIT0016","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1007\/s12098-009-0208-9","article-title":"Discordance for congenital toxoplasmosis in twins","volume":"76","author":"Thapa","year":"2009","journal-title":"Indian J Pediatr"},{"key":"2020051111581129700_CIT0017","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/0378-3782(88)90049-7","article-title":"On the unpredictable development of congenital cytomegalovirus infection. A study in twins","volume":"18","author":"Ahlfors","year":"1988","journal-title":"Early Hum Dev"},{"key":"2020051111581129700_CIT0018","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1111\/j.1365-2559.2008.02964.x","article-title":"Involvement of Hofbauer cells and maternal T cells in villitis of unknown aetiology","volume":"52","author":"Kim","year":"2008","journal-title":"Histopathology"},{"key":"2020051111581129700_CIT0019","first-page":"9.2628","article-title":"Hofbauer cells: their role in healthy and complicated pregnancy","volume":"15","author":"Reyes","year":"2018","journal-title":"Front Immunol"},{"key":"2020051111581129700_CIT0020","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.humpath.2004.01.015","article-title":"Histologic correlates of viral and bacterial infection of the placenta associated with severe morbidity and mortality in the newborn","volume":"35","author":"Satosar","year":"2004","journal-title":"Hum Pathol"},{"key":"2020051111581129700_CIT0021","doi-asserted-by":"crossref","first-page":"43","DOI":"10.5858\/arpa.2016-0401-OA","article-title":"Placental pathology of Zika virus: viral infection of the placenta induces villous stromal macrophage (Hofbauer Cell) proliferation and hyperplasia","volume":"141","author":"Rosenberg","year":"2017","journal-title":"Arch Pathol Lab Med"},{"key":"2020051111581129700_CIT0022","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.chom.2016.05.015","article-title":"Zika virus infects human placental macrophages","volume":"20","author":"Quicke","year":"2016","journal-title":"Cell Host Microbe"},{"key":"2020051111581129700_CIT0023","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.chom.2016.07.002","article-title":"Zika virus targets different primary human placental cells, suggesting two routes for vertical transmission","volume":"20","author":"Tabata","year":"2016","journal-title":"Cell Host Microbe"},{"key":"2020051111581129700_CIT0024","doi-asserted-by":"crossref","first-page":"698","DOI":"10.5858\/arpa.2015-0225-CC","article-title":"Sampling and definitions of placental lesions: amsterdam placental workshop group consensus statement","volume":"140","author":"Khong","year":"2016","journal-title":"Arch Pathol Lab Med"},{"key":"2020051111581129700_CIT0025","doi-asserted-by":"crossref","first-page":"273","DOI":"10.2350\/10-07-0872-OA.1","article-title":"Clinical associations with a placental diagnosis of delayed villous maturation: a retrospective study","volume":"14","author":"Higgins","year":"2011","journal-title":"Pediatr Dev Pathol"},{"key":"2020051111581129700_CIT0026","doi-asserted-by":"crossref","first-page":"S21","DOI":"10.1016\/j.ajog.2015.05.056","article-title":"Classification of placental lesions","volume":"213","author":"Redline","year":"2015","journal-title":"Am J Obstet Gynecol"},{"key":"2020051111581129700_CIT0027","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1016\/j.jpeds.2013.06.010","article-title":"Placental pathology in full-term infants with hypoxic-ischemic neonatal encephalopathy and association with magnetic resonance imaging pattern of brain injury","volume":"163","author":"Harteman","year":"2013","journal-title":"J Pediatr"},{"key":"2020051111581129700_CIT0028","doi-asserted-by":"crossref","first-page":"5134","DOI":"10.1128\/JVI.03674-14","article-title":"Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta","volume":"89","author":"Tabata","year":"2015","journal-title":"J Virol"},{"key":"2020051111581129700_CIT0029","doi-asserted-by":"crossref","first-page":"S8","DOI":"10.1016\/j.placenta.2017.04.020","article-title":"Congenital cytomegalovirus infection undermines early development and functions of the human placenta","volume":"59","author":"Pereira","year":"2017","journal-title":"Placenta"}],"container-title":["The Journal of Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jid\/advance-article-pdf\/doi\/10.1093\/infdis\/jiz629\/31493175\/jiz629.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jid\/article-pdf\/221\/11\/1838\/33202344\/jiz629.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jid\/article-pdf\/221\/11\/1838\/33202344\/jiz629.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T23:03:16Z","timestamp":1589238196000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jid\/article\/221\/11\/1838\/5644109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,27]]},"references-count":29,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2019,11,27]]},"published-print":{"date-parts":[[2020,5,11]]}},"URL":"https:\/\/doi.org\/10.1093\/infdis\/jiz629","relation":{},"ISSN":["0022-1899","1537-6613"],"issn-type":[{"type":"print","value":"0022-1899"},{"type":"electronic","value":"1537-6613"}],"subject":[],"published-other":{"date-parts":[[2020,6,1]]},"published":{"date-parts":[[2019,11,27]]}}}