{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T08:07:35Z","timestamp":1787731655347,"version":"build-2784847793"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,1,10]],"date-time":"2020-01-10T00:00:00Z","timestamp":1578614400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2012-37988-C04-04"],"award-info":[{"award-number":["AGL2012-37988-C04-04"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2016-78054-R"],"award-info":[{"award-number":["AGL2016-78054-R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["BES-2010\u2013040922"],"award-info":[{"award-number":["BES-2010\u2013040922"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["INIA-FPI-SGIT-2015-02"],"award-info":[{"award-number":["INIA-FPI-SGIT-2015-02"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000354","name":"Food Standards Agency","doi-asserted-by":"publisher","award":["FS231051"],"award-info":[{"award-number":["FS231051"]}],"id":[{"id":"10.13039\/501100000354","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Although experimental transmission of bovine spongiform encephalopathy (BSE) to pigs and transgenic mice expressing pig cellular prion protein (PrPC) (porcine PrP [PoPrP]\u2013Tg001) has been described, no natural cases of prion diseases in pig were reported. This study analyzed pig-PrPC susceptibility to different prion strains using PoPrP-Tg001 mice either as animal bioassay or as substrate for protein misfolding cyclic amplification (PMCA). A panel of isolates representatives of different prion strains was selected, including classic and atypical\/Nor98 scrapie, atypical-BSE, rodent scrapie, human Creutzfeldt-Jakob-disease and classic BSE from different species. Bioassay proved that PoPrP-Tg001-mice were susceptible only to the classic BSE agent, and PMCA results indicate that only classic BSE can convert pig-PrPC into scrapie-type PrP (PrPSc), independently of the species origin. Therefore, conformational flexibility constraints associated with pig-PrP would limit the number of permissible PrPSc conformations compatible with pig-PrPC, thus suggesting that pig-PrPC may constitute a paradigm of low conformational flexibility that could confer high resistance to the diversity of prion strains.<\/jats:p>","DOI":"10.1093\/infdis\/jiz646","type":"journal-article","created":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T12:09:30Z","timestamp":1576843770000},"page":"1103-1112","source":"Crossref","is-referenced-by-count":17,"title":["Porcine Prion Protein as a Paradigm of Limited Susceptibility to Prion Strain Propagation"],"prefix":"10.1093","volume":"223","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6719-9902","authenticated-orcid":false,"given":"Juan Carlos","family":"Espinosa","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (CISA-INIA), Valdeolmos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Mar\u00edn-Moreno","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (CISA-INIA), Valdeolmos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Aguilar-Calvo","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (CISA-INIA), Valdeolmos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie L","family":"Benestad","sequence":"additional","affiliation":[{"name":"Norwegian Veterinary Institute, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Andreoletti","sequence":"additional","affiliation":[{"name":"UMR Institut National de la Recherche Agronomique (INRA)\/\u00c9cole Nationale V\u00e9t\u00e9rinaire de Toulouse (ENVT) 1225, Interactions H\u00f4tes Agents Pathog\u00e8nes, Ecole Nationale V\u00e9t\u00e9rinaire de Toulouse, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Mar\u00eda","family":"Torres","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (CISA-INIA), Valdeolmos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,1,9]]},"reference":[{"key":"2021032911262422300_CIT0001","doi-asserted-by":"crossref","first-page":"13363","DOI":"10.1073\/pnas.95.23.13363","article-title":"Prions","volume":"95","author":"Prusiner","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2021032911262422300_CIT0002","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.virusres.2014.11.026","article-title":"Prion and prion-like diseases in animals","volume":"207","author":"Aguilar-Calvo","year":"2015","journal-title":"Virus 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