{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T04:02:42Z","timestamp":1782014562689,"version":"3.54.5"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T00:00:00Z","timestamp":1583280000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18H04066"],"award-info":[{"award-number":["18H04066"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17H04376"],"award-info":[{"award-number":["17H04376"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The sublingual mucosa (SLM) in the oral cavity is utilized as the site for sublingual immunotherapy to induce tolerance against allergens. We previously reported that CD206+ round-type macrophage-like cells were induced in the SLM after repeated antigen (e.g. cedar pollen or fluorescein isothiocyanate (FITC))-painting. In this study, we examined the phenotypic and functional properties of CD206+ cells induced by repeated FITC-painting on the SLM. CD206+ cells after the repeated FITC-painting possessed a macrophage-like CD11b+Ly6C+ F4\/80+CD64+ phenotype and expressed TIM-4, which was expressed in tolerogenic tissue-resident macrophages, at a high level. SLM CD206+ cells preferentially expressed molecules related to endocytosis and homeostatic processes, including the novel B7 family of immune checkpoint molecules, as assessed by microarray analyses. SLM CD206+ cells showed preferential expression of M2-related genes such as Fizz1, Aldh1a1 and Aldh1a2 but not Ym-1 and Arginase-1. A CD206+ cell-rich status inhibited OVA-specific CD4+ T-cell responses but reciprocally enhanced the proportion of both IL-10+CD4+ cells and Foxp3+ regulatory T-cells in regional lymph nodes. Co-culture of CD206+ cells with dendritic cells (DCs) showed that IL-12 production was suppressed in DCs concurrent with the decline of the MHC class IIhiCD86+ population, which was restored by neutralization of IL-10. These results demonstrate SLM CD206+ cells show the feature of tolerogenic macrophages and down-regulate the antigen-presenting cell function of mature DCs resulting in the inhibition of CD4+ T-cell responses.<\/jats:p>","DOI":"10.1093\/intimm\/dxaa014","type":"journal-article","created":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T20:16:36Z","timestamp":1582661796000},"page":"509-518","source":"Crossref","is-referenced-by-count":13,"title":["Tolerogenic properties of CD206+ macrophages appeared in the sublingual mucosa after repeated antigen-painting"],"prefix":"10.1093","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9002-9760","authenticated-orcid":false,"given":"Yue","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Molecular Immunology, Tokyo Medical and Dental University , Bunkyo-ku, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigenori","family":"Nagai","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Tokyo Medical and Dental University , Bunkyo-ku, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siwen","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Tokyo Medical and Dental University , Bunkyo-ku, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulong","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Tokyo Medical and Dental University , Bunkyo-ku, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Kawano","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Tokyo Medical and Dental University , Bunkyo-ku, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kensuke","family":"Miyake","sequence":"additional","affiliation":[{"name":"Department of Immune Regulation, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , Bunkyo-ku, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Karasuyama","sequence":"additional","affiliation":[{"name":"Department of Immune Regulation, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , Bunkyo-ku, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miyuki","family":"Azuma","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Tokyo Medical and Dental University , Bunkyo-ku, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,3,4]]},"reference":[{"key":"2022070716464864900_CIT0001","doi-asserted-by":"crossref","first-page":"149","DOI":"10.2332\/allergolint.08-RAI-0072","article-title":"Sublingual immunotherapy for Japanese cedar pollinosis","volume":"58","author":"Okubo","year":"2009","journal-title":"Allergol. 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