{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T17:54:31Z","timestamp":1780941271192,"version":"3.54.1"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T00:00:00Z","timestamp":1602028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Platform Project for Supporting Drug Discovery and Life Science Research"},{"name":"Basis for Supporting Innovative Drug Discovery and Life Science Research","award":["#18am0101121"],"award-info":[{"award-number":["#18am0101121"]}]},{"name":"Basis for Supporting Innovative Drug Discovery and Life Science Research","award":["#19am0101121"],"award-info":[{"award-number":["#19am0101121"]}]},{"DOI":"10.13039\/501100001691","name":"Grants-in-Aid for Scientific Research","doi-asserted-by":"crossref","award":["(#25430099"],"award-info":[{"award-number":["(#25430099"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012016","name":"Nipponham Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012016","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008642","name":"Foundation for Dietary Scientific Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008642","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Food allergy is a common disease caused by intake of allergen-containing foods, such as milk, eggs, peanuts and wheat. Systemic anaphylaxis is a severe hypersensitive allergic reaction resulting from degranulation of mast cells or basophils after cross-linking of surface high-affinity IgE receptors (Fc\u03b5-RI) with allergen-specific IgE and allergens. In this study, we developed a novel human mast cell\/basophil-engrafted mouse model that recapitulates systemic anaphylaxis triggered by \u03b2-lactoglobulin (BLG), a major allergen found in cow\u2019s milk. Human CD34+ hematopoietic stem cells were transferred into NOG (non-Tg) or NOG hIL-3\/hGM-CSF transgenic (Tg) mice. After 14\u201316 weeks, bovine BLG-specific human IgE was intravenously injected into humanized mice, followed by intravenous or oral bovine BLG exposure 1 day later. Body temperature in Tg, but not in non-Tg, mice gradually decreased within 10 min, and 80% of Tg mice died within 1 h by intravenous BLG exposure. Serum histamine levels and anaphylaxis scores in Tg mice were markedly increased compared to non-Tg mice. Furthermore, these allergic symptoms were significantly inhibited by epinephrine treatment of the Tg mice. Therefore, the current NOG hIL-3\/hGM-CSF Tg mouse model may be useful for development of novel anaphylaxis drugs for treatment of food allergies and for safety assessment of low-allergenicity extensively hydrolyzed cow\u2019s milk whey protein-based infant formulas.<\/jats:p>","DOI":"10.1093\/intimm\/dxaa067","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T12:17:13Z","timestamp":1601554633000},"page":"183-189","source":"Crossref","is-referenced-by-count":5,"title":["Bovine \u03b2-lactoglobulin-induced passive systemic anaphylaxis model using humanized NOG hIL-3\/hGM-CSF transgenic mice"],"prefix":"10.1093","volume":"33","author":[{"given":"Ryoji","family":"Ito","sequence":"first","affiliation":[{"name":"Central Institute for Experimental Animals , Kawasaki, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikumi","family":"Katano","sequence":"additional","affiliation":[{"name":"Central Institute for Experimental Animals , Kawasaki, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iyo","family":"Otsuka","sequence":"additional","affiliation":[{"name":"Central Institute for Experimental Animals , Kawasaki, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Central Institute for Experimental Animals , Kawasaki, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3706-2454","authenticated-orcid":false,"given":"Hiroshi","family":"Suemizu","sequence":"additional","affiliation":[{"name":"Central Institute for Experimental Animals , Kawasaki, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamoru","family":"Ito","sequence":"additional","affiliation":[{"name":"Central Institute for Experimental Animals , Kawasaki, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J","family":"Simons","sequence":"additional","affiliation":[{"name":"Polpharma Biologics B.V. , Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,10,7]]},"reference":[{"key":"2022070714070843600_CIT0001","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.jaci.2017.06.003","article-title":"The pathophysiology of anaphylaxis","volume":"140","author":"Reber","year":"2017","journal-title":"J. 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