{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T03:54:12Z","timestamp":1778903652743,"version":"3.51.4"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T00:00:00Z","timestamp":1602028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["S1411037"],"award-info":[{"award-number":["S1411037"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20K08850"],"award-info":[{"award-number":["JP20K08850"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Extracellular ATP is known to promote Th17 cell differentiation in the intestinal lamina propria by stimulating CD70+CD11clow dendritic cells (DCs) via P2X receptors (P2XRs). Recent studies have also shown that Th17 cells enhance antitumor immunity by directly promoting proliferation of cytotoxic T lymphocytes (CTLs). These finding led us to test a P2XR agonist, \u03b1\u03b2-methylene ATP (\u03b1\u03b2-ATP), as a mucosal vaccine adjuvant to promote CTL responses through Th17 induction. We demonstrated that (i) CD70+CD11clow DCs were present in the nasal lamina propria and expressed P2X1R, P2X2R and P2X4R; (ii) CD70+CD11clow DCs isolated from the nasal lamina propria enhanced Th17 cell differentiation of cocultured splenic CD4+ T cells upon stimulation with \u03b1\u03b2-ATP; (iii) mice intranasally immunized with ovalbumin (OVA) and \u03b1\u03b2-ATP had increased OVA-specific Th17 cells and CTLs in the nasal lamina propria and regional lymph nodes; (iv) mice intranasally immunized with OVA and \u03b1\u03b2-ATP also had elevated resistance to E.G7-OVA tumor growth compared with those intranasally immunized with OVA alone; (v) suramin, a broad-range inhibitor of P2 receptors, suppressed the increases of OVA-specific Th17 cells and CTLs in mice intranasally immunized with OVA and \u03b1\u03b2-ATP; and (vi) suramin also abrogated the enhanced antitumor immunity of mice intranasally immunized with OVA and \u03b1\u03b2-ATP against E.G7-OVA. Collectively, \u03b1\u03b2-ATP may be a promising mucosal adjuvant that promotes antigen-specific CTL responses via CD70+CD11clow DC-mediated Th17 induction.<\/jats:p>","DOI":"10.1093\/intimm\/dxaa068","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T12:17:13Z","timestamp":1601554633000},"page":"49-55","source":"Crossref","is-referenced-by-count":11,"title":["P2X receptor agonist enhances tumor-specific CTL responses through CD70+ DC-mediated Th17 induction"],"prefix":"10.1093","volume":"33","author":[{"given":"Shinya","family":"Yamamoto","sequence":"first","affiliation":[{"name":"Division of Chemotherapy, Kindai University Faculty of Pharmacy , Higashi-osaka, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiko","family":"Matsuo","sequence":"additional","affiliation":[{"name":"Division of Chemotherapy, Kindai University Faculty of Pharmacy , Higashi-osaka, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sho","family":"Sakai","sequence":"additional","affiliation":[{"name":"Division of Chemotherapy, Kindai University Faculty of Pharmacy , Higashi-osaka, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itsuki","family":"Mishima","sequence":"additional","affiliation":[{"name":"Division of Chemotherapy, Kindai University Faculty of Pharmacy , Higashi-osaka, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuta","family":"Hara","sequence":"additional","affiliation":[{"name":"Division of Chemotherapy, Kindai University Faculty of Pharmacy , Higashi-osaka, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Oiso","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Kindai University Faculty of Medicine , Osakasayama, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Kawada","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Kindai University Faculty of Medicine , Osakasayama, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Yoshie","sequence":"additional","affiliation":[{"name":"Emeritus professor, Kindai University , OsakaSayama, Osaka, Japan"},{"name":"Health and Kampo Institute , Sendai, Miyagi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Division of Chemotherapy, Kindai University Faculty of Pharmacy , Higashi-osaka, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,10,7]]},"reference":[{"key":"2022070716062459500_CIT0001","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1084\/jem.20041257","article-title":"IL-23 drives a pathogenic T cell population that induces autoimmune inflammation","volume":"201","author":"Langrish","year":"2005","journal-title":"J. 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