{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:27:23Z","timestamp":1791188843288,"version":"4.1.0"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2012,7,23]],"date-time":"2012-07-23T00:00:00Z","timestamp":1343001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>AbstractToll-like receptor 5 (TLR5), a sensor for bacterial flagellin, mounts innate and adaptive immune responses, and has been implicated in infectious diseases, colitis and metabolic syndromes. Although TLR5 is believed to belong to cell surface TLRs, cell surface expression has never been verified. Moreover, it has remained unclear which types of immune cells express TLR5 and contribute to flagellin-dependent responses. In this study we established an anti-mouse TLR5 monoclonal antibody and studied the cell surface expression of TLR5 on immune cells. The macrophage cell line J774 expressed endogenous TLR5 on the cell surface and produced IL-6 and G-CSF in response to flagellin. Cell surface expression of TLR5 and flagellin-induced responses were completely abolished by silencing a TLR-specific chaperone protein associated with TLR4 A (PRAT4A), demonstrating that TLR5 is another client of PRAT4A. In the in vivo immune cells, cell surface TLR5 was mainly found on neutrophils and CD11bhiLy6Chiclassical monocytes in the bone marrow, circulation, spleen and inflammatory lesions. Ly6Chiclassical monocytes, but not neutrophils, produced cytokines in response to flagellin. Splenic CD8\u2212CD4+conventional dendritic cells and CD11chiCD11bhilamina propria DCs, also clearly expressed cell surface TLR5. Collectively, cell surface expression of TLR5 is dependent on PRAT4A and restricted to neutrophils, classical monocytes and specific DC subsets.<\/jats:p>","DOI":"10.1093\/intimm\/dxs068","type":"journal-article","created":{"date-parts":[[2012,7,27]],"date-time":"2012-07-27T07:50:50Z","timestamp":1343375450000},"page":"613-623","source":"Crossref","is-referenced-by-count":45,"title":["PRAT4A-dependent expression of cell surface TLR5 on neutrophils, classical monocytes and dendritic cells"],"prefix":"10.1093","volume":"24","author":[{"given":"Takuma","family":"Shibata","sequence":"first","affiliation":[{"name":"Division of Infectious Genetics, Department of Microbiology and Immunology 1 , University of Tokyo, Minatoku, Tokyo108-8639, Japan"},{"name":"Laboratory of Innate Immunity, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, University of Tokyo 2 Minatoku, Tokyo108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Takemura","sequence":"additional","affiliation":[{"name":"Laboratory of Host Defense, World Premier International Immunology Frontier Research Center 3 , Osaka University, Suita, Osaka 565-0871, Japan"},{"name":"Department of Host Defense, Research Institute for Microbial Diseases, Osaka University 4 Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Motoi","sequence":"additional","affiliation":[{"name":"Division of Infectious Genetics, Department of Microbiology and Immunology 1 , University of Tokyo, Minatoku, Tokyo108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Goto","sequence":"additional","affiliation":[{"name":"Division of Mucosal Immunology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo 5 Minatoku, Tokyo108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thangaraj","family":"Karuppuchamy","sequence":"additional","affiliation":[{"name":"Laboratory of Host Defense, World Premier International Immunology Frontier Research Center 3 , Osaka University, Suita, Osaka 565-0871, Japan"},{"name":"Department of Host Defense, Research Institute for Microbial Diseases, Osaka University 4 Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kumi","family":"Izawa","sequence":"additional","affiliation":[{"name":"Division of Cellular Therapy, 6 Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo,Minatoku, Tokyo108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobing","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Infectious Genetics, Department of Microbiology and Immunology 1 , University of Tokyo, Minatoku, Tokyo108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachiko","family":"Akashi-Takamura","sequence":"additional","affiliation":[{"name":"Division of Infectious Genetics, Department of Microbiology and Immunology 1 , University of Tokyo, Minatoku, Tokyo108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natsuko","family":"Tanimura","sequence":"additional","affiliation":[{"name":"Division of Infectious Genetics, Department of Microbiology and Immunology 1 , University of Tokyo, Minatoku, Tokyo108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Kunisawa","sequence":"additional","affiliation":[{"name":"Division of Mucosal Immunology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo 5 Minatoku, Tokyo108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kiyono","sequence":"additional","affiliation":[{"name":"Division of Mucosal Immunology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo 5 Minatoku, Tokyo108-8639, Japan"},{"name":"Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency 7 Chiyodaku, Tokyo 102-8666, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizuo","family":"Akira","sequence":"additional","affiliation":[{"name":"Laboratory of Host Defense, World Premier International Immunology Frontier Research Center 3 , Osaka University, Suita, Osaka 565-0871, Japan"},{"name":"Department of Host Defense, Research Institute for Microbial Diseases, Osaka University 4 Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshio","family":"Kitamura","sequence":"additional","affiliation":[{"name":"Division of Cellular Therapy, 6 Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo,Minatoku, Tokyo108-8639, Japan"},{"name":"Division of Stem Cell Signaling, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo 8 Minatoku, Tokyo108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiro","family":"Kitaura","sequence":"additional","affiliation":[{"name":"Division of Cellular Therapy, 6 Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo,Minatoku, Tokyo108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Uematsu","sequence":"additional","affiliation":[{"name":"Laboratory of Host Defense, World Premier International Immunology Frontier Research Center 3 , Osaka University, Suita, Osaka 565-0871, Japan"},{"name":"Department of Host Defense, Research Institute for Microbial Diseases, Osaka University 4 Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kensuke","family":"Miyake","sequence":"additional","affiliation":[{"name":"Division of Infectious Genetics, Department of Microbiology and Immunology 1 , University of Tokyo, Minatoku, Tokyo108-8639, Japan"},{"name":"Laboratory of Innate Immunity, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, University of Tokyo 2 Minatoku, Tokyo108-8639, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,7,23]]},"reference":[{"key":"2023120117272710100_CIT0001","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1146\/annurev.immunol.24.021605.090552","article-title":"Genetic analysis of host resistance: toll-like receptor signaling and immunity at 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